1.7.99.1: hydroxylamine reductase

This is an abbreviated version!
For detailed information about hydroxylamine reductase, go to the full flat file.

Reaction

Synonyms

CcuHao, CfHao, CmHao, epsilonHao, epsilonproteobacterial hydroxylamine oxidoreductase, fuscoredoxin, haoA, HAOr, HCP, hybrid cluster protein, Kustc0458, More, NpHao, prismane protein, reductase, hydroxylamine (acceptor)

ECTree

     1 Oxidoreductases

         1.7 Acting on other nitrogenous compounds as donors

             1.7.99 With unknown physiological acceptors

                1.7.99.1 hydroxylamine reductase

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Results	in table
3	Activating Compound
9540	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
6	Cofactor
1	Crystallization (Commentary)
4	General Information
17	Inhibitors
1	kcat/KM [mM/s]
21	KM Value [mM]
1	Localization
9	Metals/Ions
11	Molecular Weight [Da]
9	Natural Substrates/ Products (Substrates)
15	Organism
4	Pathway
20	PDB ID
8	pH Optimum
6	Protein Variants
11	Purification (Commentary)
1	Reaction
3	Reaction Type
15	Reference
4	Source Tissue
22	Specific Activity [micromol/min/mg]
2	Storage Stability
32	Substrates and Products (Substrate)
2	Subunits
28	Synonyms
1	Systematic Name
5	Temperature Optimum [°C]
3	Temperature Stability [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.7.99.1 - hydroxylamine reductase

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SUBSTRATE

PRODUCT                       

REACTION DIAGRAM

ORGANISM

UNIPROT

COMMENTARY
(Substrate)

LITERATURE
(Substrate)

COMMENTARY
(Product)

LITERATURE
(Product)

Reversibility
r=reversible
ir=irreversible
?=not specified

hydroxylamine + ferricytochrome c

nitrite + ferrocytochrome c

4 entries

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

6 entries

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

10 entries

hydroxylamine + reduced methyl viologen

ammonia + oxidized methyl viologen

Pyrococcus furiosus

-

-

712507

-

-

?

hydroxylamine + reduced methyl viologen

NH3 + H2O + oxidized methyl viologen

Candidatus Kuenenia stuttgartiensis

-

-

765075

-

-

?

nitrate + FADH2

nitrite + FAD + H2O

Pseudomonas aeruginosa

-

-

394261

-

-

r

nitrate + reduced benzyl viologen

nitrite + H2O + oxidized benzyl viologen

Cucurbita pepo

-

-

394269

-

-

?

nitrate + reduced methylene blue

nitrite + H2O + oxidized methylene blue

Pseudomonas aeruginosa

-

-

394261

-

-

r

nitrate + reduced pyocyanine

nitrite + H2O + oxidized pyocyanine

Pseudomonas aeruginosa

-

-

394261

-

-

r

additional information

?

-

5 entries

hydroxylamine + ferricytochrome c

nitrite + ferrocytochrome c

Caminibacter mediatlanticus

-

-

743303

-

-

r

hydroxylamine + ferricytochrome c

nitrite + ferrocytochrome c

Campylobacter curvus

A7GXH2

-

743303

-

-

r

hydroxylamine + ferricytochrome c

nitrite + ferrocytochrome c

Campylobacter fetus subsp. fetus

-

-

743303

-

-

r

hydroxylamine + ferricytochrome c

nitrite + ferrocytochrome c

Nautilia profundicola

-

-

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Caminibacter mediatlanticus

-

-

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Caminibacter mediatlanticus

-

best substrate

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Campylobacter curvus

A7GXH2

-

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Campylobacter fetus subsp. fetus

-

-

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Nautilia profundicola

-

-

743303

-

-

r

hydroxylamine + ferrocytochrome c

NH3 + H2O + ferricytochrome c

Nautilia profundicola

-

best substrate

743303

-

-

r

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Cucurbita pepo

-

hydroxylamine reductase 1 reacts with reduced benzyl viologen, ferredoxin and FMNH2

394265, 394267

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Cucurbita pepo

-

FADH2, NADPH

394265

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Cucurbita pepo

-

reduced benzyl viologen as electron donor

394269

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Cucurbita pepo

-

hydroxylamine reductase 2 reacts with benzyl viologen and dithionite

394267

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Escherichia coli

-

-

394266

-

394266

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Pseudomonas aeruginosa

-

with pyocyanine or reduced methylene blue as electron donor

394261

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Spinacia oleracea

-

hydroxylamine reductase 1 reacts with reduced benzyl viologen, ferredoxin and FMNH2

394265

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Spinacia oleracea

-

hydroxylamine reductase 2 reacts with reduced benzyl viologen

394265

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Spinacia oleracea

-

FADH2, NADPH

394265

-

-

?

hydroxylamine + reduced acceptor

ammonia + oxidized acceptor

Triticum aestivum

-

methyl viologen is more effective than benzyl viologen, flavin nucleotides are also utilized but to a lesser extent

394268

-

-

?

additional information

?

-

Caminibacter mediatlanticus

-

the recombinant enzyme is able to catalyze reduction of nitrite (yielding ammonium), EC 1.7.2.2, and hydroxylamine whereas hydroxylamine oxidation is negligible

743303

-

-

?

additional information

?

-

Campylobacter curvus

A7GXH2

the recombinant enzyme is able to catalyze reduction of nitrite (yielding ammonium), EC 1.7.2.2, and hydroxylamine whereas hydroxylamine oxidation is negligible

743303

-

-

?

additional information

?

-

Campylobacter fetus subsp. fetus

-

the recombinant enzyme is able to catalyze reduction of nitrite (yielding ammonium), EC 1.7.2.2, and hydroxylamine whereas hydroxylamine oxidation is negligible

743303

-

-

?

additional information

?

-

Candidatus Kuenenia stuttgartiensis

-

the enzyme is not able to oxidize hydroxylamine or hydrazine

765075

-

-

-

additional information

?

-

Nautilia profundicola

-

the recombinant enzyme is able to catalyze reduction of nitrite (yielding ammonium), EC 1.7.2.2, and hydroxylamine whereas hydroxylamine oxidation is negligible

743303

-

-

?