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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
+
H2O
=
5-hydroxyisourate
+
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Temperature Stability

Temperature Stability on EC 1.7.3.3 - factor-independent urate hydroxylase

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TEMPERATURE STABILITY
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0 - 70
-
the enzyme remains stable (more than 90% activity) for 30 min at 0-20°C. The activity drops to about 60, 50, 40, less than 30, and less than 20% after 30 min at 30, 40, 50, 60, and 70°C, respectively. At 40°C, the enzyme shows about 80% activity after 5 min, about 65% activity after 10 min, about 50% activity after 20-40 min, and about 30% activity after 50-60 min
10 - 80
-
about 80% relative activity at 10°C, about 85% relative activity at 20-30°C, about 90% relative activity at 37°C, about 40% relative activity at 40°C, about 30% relative activity at 50°C, about 20% relative activity at 60°C, about 10% relative activity at 70°C, and no activity at 80°C after 30 min incubation
100
-
35% relative activity at 100°C
18 - 20
-
2 days, 70% loss of activity
30 - 40
-
enzyme activity is diminished 60% when it is incubated at 30°C for 10 min. After 5 min at 40°C, there is a 25% decline in enzyme activity. The enzyme loses almost 60% of its original activity after 40 min at 40°C, whereas more than 50% of enzyme activity is preserved in the presence of lactose. Half-life at 40°C is almost 38 min and addition of raffinose does not change the half-life, whereas the presence of lactose has remarkable impact on enzyme half-life (46 min). Lactose notably enhances the melting temperature from 27 to 37°C
35 - 60
-
the enzyme is stable at 35ºC for 1 h and thermally inactivated at 60°C after 1 h. Rising temperature up to 40°C diminishes the enzyme activity to 83% and 80% after 45 and 60 min, respectively
52
-
uricase covalently linked to monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase and branched monomethoxypoly(ethylene glycol) N-leucine-OSu-uricase 50% loss of activity
55
-
uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 and 0.1 mg/ml shows no loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.01 mg/ml shows about 35% loss of activity after 5 h at 55°C. Uricase entrapped in lipid vesicles at enzyme concentrations of 0.005 mg/ml shows about 50% loss of activity after 5 h at 55°C. The free uricase at 0.005 mg/ml is rapidly deactivated to about 30% of the initial activity within an incubation time of 2 h, while more than 70% of the initial enzyme activity remains for the uricase at 0.1 mg/ml in the identical incubation time
60 - 61
-
native uricase and poly(N-acryloylmorpholine)-OSu-uricase 50% loss of activity
61
melting temperature
75
DQ887577
enzyme is thermostable, after heat treatment at 75°C for 45 min, the uricase retains about 100% of its initial activity
80
DQ887577
70% of initial activity remains after 45 min at 80°C
additional information