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1.7.3.3: factor-independent urate hydroxylase

This is an abbreviated version!
For detailed information about factor-independent urate hydroxylase, go to the full flat file.

Word Map on EC 1.7.3.3

Reaction

Urate
+
O2
+
H2O
=
5-hydroxyisourate
+
H2O2

Synonyms

AaUO, AgUOX, dHU-wPU, ELITEK, Fasturtec, MVSM, N-35, Nodule specific uricase, Nodulin 35, Nodulin 35 homolog, Non-symbiotic uricase, oxidase, urate, Pucl, Rasburicase, Uaz, Uox, Urate oxidase, urate oxidoreductase, UriA, uric acid oxidase, uricase, uricase II, Uricoenzyme, Uricozyme

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.3 With oxygen as acceptor
                1.7.3.3 factor-independent urate hydroxylase

Purification

Purification on EC 1.7.3.3 - factor-independent urate hydroxylase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
ammonium sulfate precipitation, DEAE cellulose column chromatography, and Sephadex G-75 gel filtration
-
ammonium sulfate precipitation, DEAE-Sephadex A50 colum chromatography, and Sephadex-G75 gel filtration
-
ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Superdex 75 gel filtration
-
ammonium sulfate precipitation, ion exchange chromatography and gel filtration
-
by anion exchange and hydropbobic interaction chromatography
by Ni-NTA affinity chromatography
-
DEAE cellulose column chromatography
DEAE Sepharose FF chromatography, Phenyl-Sepharose FF chromatography and HiLoad 26/60 Superdex 75 gel filtration
-
DEAE-cellulose column chromatography
enzyme synthesized with C-terminal 6-histidine tag
-
HiTrap Q column chromatography and Superdex 200 gel filtration
-
native enzyme 19.7fold by ammonium sulfate fractionation, anion exchange exchange and hydrophobic interaction chromatography, and gel filtration
-
Ni-NTA agarose affinity column chromatography
-
Ni-NTA agarose column chromatography, and Superdex 200 gel filtration
-
Ni-NTA column chromatography
-
Ni-NTA column chromatography and Sephacryl S-300 gel filtration
nickel resin column chromatography and Superdex 200 gel filtration
recombinant enzyme
-
Superdex S200PG gel filtration
uricase-MBP fusion protein
-