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1.7.2.5: nitric oxide reductase (cytochrome c)

This is an abbreviated version!
For detailed information about nitric oxide reductase (cytochrome c), go to the full flat file.

Word Map on EC 1.7.2.5

Reaction

nitrous oxide
+
2 ferricytochrome c
 +
H2O
= 2 nitric oxide + 2 ferrocytochrome c + 2 H+

Synonyms

Anor, ba3-oxidase, c-type nitric oxide reductase, cNOR, Cnor1, Cnor2, cytochrome c dependent nitric oxide reductase, cytochrome c dependent NOR, cytochrome c type NOR, cytochrome c-dependent nitric oxide reductase, cytochrome c-dependent NO reductase, EC 1.7.99.7, flavorubredoxin, FlRd, Fnor, FprA, membrane-integrated nitric oxide reductase, nitric oxide reductase, nitric oxide reductase cytochrome, nitric oxide-reductase, nitrogen oxide reductase, NO reductase, NO-reductase, NOR, NorB, NorBC, NorC, NorCB, NorZ, P450nor, qCuANOR, respiratory nitric oxide reductase, respiratory NO reductase, S-NOR, scavenging nitric oxide reductase, Tnor

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.5 nitric oxide reductase (cytochrome c)

Engineering

Engineering on EC 1.7.2.5 - nitric oxide reductase (cytochrome c)

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PROTEIN VARIANTS
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
A239K
-
6% activity of modified soluble wild-type enzyme
A242K
-
42% activity of modified soluble wild-type enzyme
G240K
-
63% activity of modified soluble wild-type enzyme
M244K
-
60% activity of modified soluble wild-type enzyme
M247K
-
130% activity of modified soluble wild-type enzyme
N241K
-
38% activity of modified soluble wild-type enzyme
N246K
-
45% activity of modified soluble wild-type enzyme
S286T
-
7% activity of wild-type
S286V
-
1% activity of wild-type
T243H
-
8% activity of modified soluble wild-type enzyme
T243K
-
12% activity of modified soluble wild-type enzyme
T243R
-
17% activity of modified soluble wild-type enzyme
H25F
-
3% of wild-type kcat
H25F/Y195F
-
strong decrease in NOR activity
Y195F
-
15% of wild-type kcat
D185A
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
D185E
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
D185N
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
E122A
-
substitution leads to an almost complete loss of NOR activity
E122D
-
substitution mutant retains 83.2% of the activity of the wild-type enzyme
E122Q
-
substitution mutant retains 1.3% of the activity of the wild-type enzyme
E125A
-
substitution leads to an almost complete loss of NOR activity
E125D
-
substitution leads to an almost complete loss of NOR activity
E125Q
-
substitution mutant retains 13.4% of the activity of the wild-type enzyme
E198A
E202A
E267A
E78F
site-directed mutagenesis, the mutant does not express
K54A
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
N47F
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
N47L
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
Q394M
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
Q398L
site-directed mutagenesis, mutation of a conserved residue involved in the proton transfer, kinetics compared to the wild-type
additional information