1.7.2.4: nitrous-oxide reductase
This is an abbreviated version!
For detailed information about nitrous-oxide reductase, go to the full flat file.
Word Map on EC 1.7.2.4
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1.7.2.4
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denitrification
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denitrify
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nitrite
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greenhouse
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denitrificans
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paracoccus
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stutzeri
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dinitrogen
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wastewater
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binuclear
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tetranuclear
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cycloclastes
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mixed-valence
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multicopper
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ammonia-oxidizing
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n-cycle
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nautica
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wolinella
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arable
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nitrospirae
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stratospheric
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agriculture
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t-rflp
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degradation
- 1.7.2.4
-
denitrification
-
denitrify
- nitrite
-
greenhouse
- denitrificans
- paracoccus
- stutzeri
-
dinitrogen
-
wastewater
-
binuclear
-
tetranuclear
- cycloclastes
-
mixed-valence
-
multicopper
-
ammonia-oxidizing
-
n-cycle
- nautica
-
wolinella
-
arable
- nitrospirae
-
stratospheric
- agriculture
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t-rflp
- degradation
Reaction
+ + = + 2 ferrocytochrome c + 2 H+
Synonyms
cNosZ, EC 1.7.99.6, HdN2OR, McoP, MhN2OR, multicopper oxidase, N(2)OR, N2O reductase, N2OR, nitrous oxide reductase, NosZ, PpN2OR, PsN2OR, Sden_2219, SdN2OR, Z-type N2OR
ECTree
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Purification
Purification on EC 1.7.2.4 - nitrous-oxide reductase
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a pink form is isolated when the purification is done aerobically, a purple form when the purification is under anaerobic conditions
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anaerobical purification of N2OR under an Ar atmosphere by three different steps of anion exchange chromatography, followed by cytochrome c affinity chromatography and ultrafiltration
DEAE cellulose column chromatography, hydroxyapatite column chromatography, Mono Q10 anion-exchange column chromatography, and Sephadex S-200 gel filtration
DEAE-FF ion exchange chromatography and Resource Q column chromatography
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isolation of the enzyme in the purple form, in which the copper centre is in the oxidized [2Cu2+:2Cu+] redox state and is redox active. Isolation of this form in the presence of oxygen from a microaerobic culture in the presence of nitrate and also from a strictly anaerobic culture
Marinobacter nauticus
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purification under anaerobic conditions
recombinant His6-tagged enzyme and Strep-tagged enzyme from Escherichia coli strain BL21(DE3) with or without bound calcium by metal affinity and streptavidin chromatography, respectively, followed by gel filtration