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1.7.2.4: nitrous-oxide reductase

This is an abbreviated version!
For detailed information about nitrous-oxide reductase, go to the full flat file.

Word Map on EC 1.7.2.4

Reaction

nitrogen
+
H2O
 +
2 ferricytochrome c
=
nitrous oxide
 + 2 ferrocytochrome c + 2 H+

Synonyms

cNosZ, EC 1.7.99.6, HdN2OR, McoP, MhN2OR, multicopper oxidase, N(2)OR, N2O reductase, N2OR, nitrous oxide reductase, NosZ, PpN2OR, PsN2OR, Sden_2219, SdN2OR, Z-type N2OR

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.2 With a cytochrome as acceptor
                1.7.2.4 nitrous-oxide reductase

Cloned

Cloned on EC 1.7.2.4 - nitrous-oxide reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of the nos gene cluster
-
codon-optimized gene nosZ, from canonical gene cluster nosRZDFYL(X), recombinant overexpression of His6-tagged enzyme and of Strep-tagged enzyme in Escherichia coli strain BL21(DE3)
expressed in Escherichia coli
-
expressed in Escherichia coli BL21(DE3) cells
Stutzerimonas stutzeri
expression as metal-free apo protein
expression in Escherichia coli
-
expression in Escherichia coli HB101, no activity
-
expression in Nicotiana tabacum
gene nosZ, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative reverse transcriptase PCR expression analysis
gene nosZ, enzyme expression analysis during agricultural waste composting process, overview
-
gene nosZ, expression as nosRZD transcript, quantitative PCR-based expression analysis
gene nosZ, transcription of nosZ takes place concomitantly with that of narG under suboxic conditions. Catalytically functional N2OR is synthesized and active in aerobically raised cells transferred to vials with 7 vol% O2 in headspace, but N2O reduction rates are 10 times higher when anaerobic precultures are subjected to the same conditions. Upon oxygen exposure, there is an incomplete and transient inactivation of N2OR that can be ascribed to its lower ability to compete for electrons compared with terminal oxidases, quantitative real-time PCR enzyme expression analysis
-
the gene encoding the enzyme in the nos cluster is cloned from a genomic library