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1.7.1.3: nitrate reductase (NADPH)

This is an abbreviated version!
For detailed information about nitrate reductase (NADPH), go to the full flat file.

Word Map on EC 1.7.1.3

Reaction

nitrite
+
NADP+
+
H2O
=
nitrate
+
NADPH
+
H+

Synonyms

assimilatory NADPH-nitrate reductase, assimilatory NADPH:nitrate reductase, Assimilatory nitrate reductase, assimilatory reduced nicotinamide adenine dinucleotide phosphate-nitrate reductase, EC 1.6.6.3, EC 1.7.99.4, MSMEG_2837, NADPH-dependent nitrate reductase, NADPH-nitrate reductase, NADPH2:nitrate oxidoreductase, NADPH:nitrate reductase, NADPH:NR, NaR1, narB, nit-3, nitrate reductase, nitrate reductase (NADPH), nitrate reductase (reduced nicotinamide adenine dinucleotide phosphate), nitrate reductase [NADPH], NR, triphosphopyridine nucleotide-nitrate reductase

ECTree

     1 Oxidoreductases
         1.7 Acting on other nitrogenous compounds as donors
             1.7.1 With NAD+ or NADP+ as acceptor
                1.7.1.3 nitrate reductase (NADPH)

Molecular Weight

Molecular Weight on EC 1.7.1.3 - nitrate reductase (NADPH)

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MOLECULAR WEIGHT
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
102000
2 * 102000
115000
-
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested
116000
-
4 * 116000
130000
-
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested
132000
-
2 * 97000: nit-3 enzyme, 2 * 132000, wild-type
145000
-
monodimer, 2 * 145000
150000
-
1 * 130000 + 1 * 115000, SDS-PAGE, homodimer of 2 * 150000 suggested
180000
180000 - 200000
-
sucrose density gradient, gel-filtration
197000
-
gel filtration
204000
214000
-
gel filtration
230000
-
sucrose density gradient, gel filtration
235000
-
sucrose density gradient centrifugation, gel filtration
272000
-
wild-type, gel filtration
38000
-
2 * 59000 + 2 * 38000, SDS-PAGE, after heat-treatment all polypeptide chains are 59 kDa
450000
-
native gel electrophoresis
59000
-
2 * 59000 + 2 * 38000, SDS-PAGE, after heat-treatment all polypeptide chains are 59 kDa
91000
-
2 * 91000, homodimer, SDS-PAGE, smaller bands are caused by proteolytic cleavage
additional information
-
gel filtration and analytical ultracentrifugation