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0.163
1,4-Naphthoquinone
-
pH 7.8, 37°C
50.2
2,6-dichlorophenolindophenol
-
-
0.003
2-hydroxyestradiol
-
pH 7.8, 37°C
0.058
2-methyl-1,4-naphthoquinone
-
pH 7.8, 37°C
0.097
9,10-phenanthrenequinone
-
pH 7.8, 37°C
33 - 417
ferricytochome b5
-
1 - 911
ferricytochrome b5
10 - 600
ferrocytochrome b5
0.017
Nitrofurantoin
-
pH 7.8, 37°C
12
ferricyanide
mutant G179V, pH 7.0
21.7
ferricyanide
-
cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein
30.8
ferricyanide
-
mutant P247L
30.8
ferricyanide
-
mutant enzyme P247L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
33
ferricyanide
mutant G179T, pH 7.0
40
ferricyanide
K110Q mutant enzyme
42
ferricyanide
mutant G179P, pH 7.0
76
ferricyanide
-
G273 mutant enzyme
77
ferricyanide
pH 7.0, 25°C,T66V mutant enzyme
83
ferricyanide
mutant Y93D, pH 7.0, 25°C
83
ferricyanide
mutant Y93H, pH 7.0, 25°C
83
ferricyanide
mutant Y93W, pH 7.0, 25°C
98.3
ferricyanide
-
enzyme from liver microsomes, protease solubilized
100
ferricyanide
P144L/L148P mutant enzyme, pH 7.0, 25°C
110
ferricyanide
-
H49E mutant enzyme
120
ferricyanide
K110E mutant enzyme
133
ferricyanide
-
mutant G75S
133
ferricyanide
mutant Y93S, pH 7.0, 25°C
187
ferricyanide
-
mutant G75S/V252M
200
ferricyanide
mutant Y93A, pH 7.0, 25°C
270
ferricyanide
K110A mutant enzyme
283
ferricyanide
P144L mutant enzyme, pH 7.0, 25°C
300
ferricyanide
L148P mutant enzyme, pH 7.0, 25°C
300
ferricyanide
-
pH 7.0, S127P mutant enzyme
337
ferricyanide
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
340
ferricyanide
K110H mutant enzyme
357
ferricyanide
-
mutant P247A
357
ferricyanide
-
mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
399
ferricyanide
-
mutant P248A
399
ferricyanide
-
mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
400
ferricyanide
-
DELTAF272 mutant enzyme
467
ferricyanide
mutant P92G, pH 7.0, 25°C
470
ferricyanide
-
H49A mutant enzyme
470
ferricyanide
K110R mutant enzyme
483
ferricyanide
mutant Y93F, pH 7.0, 25°C
490
ferricyanide
-
H49K mutant enzyme
504
ferricyanide
-
mutant P248L
504
ferricyanide
-
mutant enzyme P248L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
517
ferricyanide
mutant P92A, pH 7.0, 25°C
559
ferricyanide
mutant G179A, pH 7.0
630
ferricyanide
-
native enzyme
633
ferricyanide
-
mutant V252M
677
ferricyanide
-
enzyme from erythrocyte membrane
684
ferricyanide
pH 7.0, 25°C,T66A mutant enzyme
700
ferricyanide
-
enzyme from erythrocyte cytosol
727
ferricyanide
-
H49Y mutant enzyme
767
ferricyanide
-
pH 7.0, 25°C
800
ferricyanide
-
wild-type
800
ferricyanide
recombinant wild-type enzyme
800
ferricyanide
wild-type, pH 7.0, 25°C
800
ferricyanide
-
pH 7.0, wild type enzyme
800
ferricyanide
wild type enzyme, pH 7.0, 25°C
800
ferricyanide
wild-type, pH 7.0
810
ferricyanide
-
recombinant wild-type enzyme
822
ferricyanide
pH 7.0, 25°C, wild type enzyme
827
ferricyanide
-
enzyme from liver microsomes, detergent solubilized
838
ferricyanide
-
mutant P249A
838
ferricyanide
-
mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
880
ferricyanide
mutant P92S, pH 7.0, 25°C
976
ferricyanide
pH 7.0, 25°C,T66S mutant enzyme
1241
ferricyanide
-
mutant P249L
1241
ferricyanide
-
mutant enzyme P249L, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
33
ferricytochome b5
P144L/L148P mutant enzyme, pH 7.0, 25°C
-
117
ferricytochome b5
P144L mutant enzyme, pH 7.0, 25°C
-
130
ferricytochome b5
L148P mutant enzyme, pH 7.0, 25°C
-
417
ferricytochome b5
wild type enzyme, pH 7.0, 25°C
-
1
ferricytochrome b5
K110E mutant enzyme
4
ferricytochrome b5
K110Q mutant enzyme
8.3
ferricytochrome b5
-
cytochrome b5/cytochrome b5 reductase FAD-domain fusion protein
14
ferricytochrome b5
mutant Y93D, pH 7.0, 25°C
19
ferricytochrome b5
mutant Y93W, pH 7.0, 25°C
21.3
ferricytochrome b5
-
-
27
ferricytochrome b5
mutant Y93H, pH 7.0, 25°C
27.2
ferricytochrome b5
-
mutant P247L
32
ferricytochrome b5
mutant Y93S, pH 7.0, 25°C
38
ferricytochrome b5
pH 7.0, 25°C,T66V mutant enzyme
57
ferricytochrome b5
-
G273 mutant enzyme
57
ferricytochrome b5
mutant Y93A, pH 7.0, 25°C
60
ferricytochrome b5
-
mutant G75S
67
ferricytochrome b5
mutant P275L, 25°C, pH 7.0
85
ferricytochrome b5
-
mutant G75S/V252M
90
ferricytochrome b5
K110A mutant enzyme
105
ferricytochrome b5
-
R63Q mutant enzyme
106
ferricytochrome b5
-
pH 7.0, S127P mutant enzyme
107
ferricytochrome b5
-
Y65A mutant enzyme
110
ferricytochrome b5
K110H mutant enzyme
121
ferricytochrome b5
-
R63A mutant enzyme
125
ferricytochrome b5
-
L41A mutant enzyme
147
ferricytochrome b5
-
wild type enzyme, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
160
ferricytochrome b5
-
H49E mutant enzyme
165
ferricytochrome b5
-
S99V mutant enzyme
167
ferricytochrome b5
-
-
167
ferricytochrome b5
-
mutant enzyme P247A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
191
ferricytochrome b5
-
K97A mutant enzyme
200
ferricytochrome b5
K110R mutant enzyme
200
ferricytochrome b5
-
mutant P247A
270
ferricytochrome b5
recombinant wild-type enzyme
270
ferricytochrome b5
-
mutant P249L
275
ferricytochrome b5
-
mutant P248L
285
ferricytochrome b5
-
mutant V252M
288
ferricytochrome b5
-
mutant P248A
295
ferricytochrome b5
-
S99A mutant enzyme
295
ferricytochrome b5
mutant Y93F, pH 7.0, 25°C
301
ferricytochrome b5
-
mutant enzyme P248A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
305
ferricytochrome b5
-
Y65F mutant enzyme
314
ferricytochrome b5
pH 7.0, 25°C,T66A mutant enzyme
315
ferricytochrome b5
mutant P92S, pH 7.0, 25°C
320
ferricytochrome b5
mutant P92A, pH 7.0, 25°C
335
ferricytochrome b5
mutant P92G, pH 7.0, 25°C
347
ferricytochrome b5
-
R63K mutant enzyme
360
ferricytochrome b5
-
wild-type
367
ferricytochrome b5
-
pH 7.0, wild type enzyme
380
ferricytochrome b5
-
H49A mutant enzyme
400
ferricytochrome b5
wild-type, pH 7.0, 25°C
415
ferricytochrome b5
-
K97R mutant enzyme
420
ferricytochrome b5
-
H49K mutant enzyme
441
ferricytochrome b5
-
mutant P249A
450
ferricytochrome b5
-
DELTAF272 mutant enzyme
472
ferricytochrome b5
-
L125A mutant enzyme
510
ferricytochrome b5
-
native enzyme
520
ferricytochrome b5
-
S99T mutant enzyme
540
ferricytochrome b5
-
mutant enzyme P249A, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
560
ferricytochrome b5
-
recombinant wild-type enzyme
563
ferricytochrome b5
-
recombinant K110M mutant enzyme
580
ferricytochrome b5
-
H49Y mutant enzyme
600
ferricytochrome b5
wild-type, 25°C, pH 7.0
661
ferricytochrome b5
-
recombinant wild-type enzyme
705
ferricytochrome b5
pH 7.0, 25°C,T66S mutant enzyme
742
ferricytochrome b5
-
recombinant K110A mutant enzyme
872
ferricytochrome b5
-
recombinant wild-type enzyme
877
ferricytochrome b5
-
recombinant K110R mutant enzyme
911
ferricytochrome b5
pH 7.0, 25°C, wild type enzyme
10
ferrocytochrome b5
mutant G179P, pH 7.0
17
ferrocytochrome b5
mutant G179V, pH 7.0
18
ferrocytochrome b5
mutant G179T, pH 7.0
245
ferrocytochrome b5
mutant G179A, pH 7.0
400
ferrocytochrome b5
wild-type, pH 7.0
600
ferrocytochrome b5
-
pH 7.0, 25°C
0.733
NADH
wild-type, cosubstrate ferricyanide, 25°C, pH 7.0
0.8
NADH
mutant P275L, cosubstrate ferricyanide, 25°C, pH 7.0
21.7
NADH
-
mutant P247L, ferricyanide as electron acceptor
21.7
NADH
-
mutant enzyme P247L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
53
NADH
-
G273 mutant enzyme
77
NADH
-
H49E mutant enzyme
100
NADH
pH 7.0, 25°C,T66V mutant enzyme
180
NADH
-
Y65A mutant enzyme
250
NADH
D239S/F251R mutant enzyme, pH 7.0, 25°C
310
NADH
-
DELTAF272 mutant enzyme
333
NADH
D239T mutant enzyme, pH 7.0, 25°C
354
NADH
-
mutant P247A, ferricyanide as electron acceptor
354
NADH
-
mutant enzyme P247A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
367
NADH
F251Y mutant enzyme, pH 7.0, 25°C
375
NADH
-
S99V mutant enzyme
403
NADH
-
R63A mutant enzyme
412
NADH
-
R63Q mutant enzyme
433
NADH
D239S mutant enzyme, pH 7.0, 25°C
435
NADH
-
S99A mutant enzyme
440
NADH
-
H49A mutant enzyme
467
NADH
D239S/F251Y mutant enzyme, pH 7.0, 25°C
500
NADH
F251R mutant enzyme, pH 7.0, 25°C
501
NADH
-
Y65F mutant enzyme
515
NADH
-
R63K mutant enzyme
517
NADH
D239E mutant enzyme, pH 7.0, 25°C
540
NADH
-
mutant P248L, ferricyanide as electron acceptor
540
NADH
-
mutant enzyme P248L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
559
NADH
-
mutant P248A, ferricyanide as electron acceptor
559
NADH
-
mutant enzyme P248A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
570
NADH
-
recombinant wild-type enzyme
681
NADH
-
mutant P249A, ferricyanide as electron acceptor
681
NADH
-
mutant enzyme P249A, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
710
NADH
-
H49K mutant enzyme
717
NADH
D239T/F251R mutant enzyme, pH 7.0, 25°C
742
NADH
-
ferricyanide as electron acceptor
742
NADH
-
wild type enzyme, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
750
NADH
-
H49Y mutant enzyme
754
NADH
-
wild-type, pH 7.0
754
NADH
-
mutant D239G, pH 7.0
754
NADH
-
mutant E255-, pH 7.0
754
NADH
-
mutant G291D, pH 7.0
800
NADH
wild type enzyme, pH 7.0, 25°C
851
NADH
-
S99T mutant enzyme
909
NADH
-
K97R mutant enzyme
984
NADH
pH 7.0, 25°C,T66A mutant enzyme
1059
NADH
-
mutant P249L, ferricyanide as electron acceptor
1059
NADH
-
mutant enzyme P249L, using ferricyanide as cosubstrate, in 100 mM potassium phosphate buffer (pH 7.0) at 25°C
1060
NADH
-
K97A mutant enzyme
1100
NADH
-
recombinant wild-type enzyme
1100
NADH
pH 7.0, 25°C, wild type enzyme
1150
NADH
pH 7.0, 25°C,T66S mutant enzyme
3
NADPH
-
mutant E255-, pH 7.0
5.2
NADPH
D239E mutant enzyme, pH 7.0, 25°C
8
NADPH
mutant G179V, pH 7.0
12
NADPH
mutant G179T, pH 7.0
17
NADPH
mutant G179P, pH 7.0
19
NADPH
-
mutant G291D, pH 7.0
33
NADPH
wild type enzyme, pH 7.0, 25°C
33
NADPH
-
wild-type, pH 7.0
33
NADPH
wild-type, pH 7.0
48
NADPH
mutant G179A, pH 7.0
50
NADPH
F251R mutant enzyme, pH 7.0, 25°C
50
NADPH
F251Y mutant enzyme, pH 7.0, 25°C
190
NADPH
-
mutant D239G, pH 7.0
200
NADPH
D239S/F251R mutant enzyme, pH 7.0, 25°C
217
NADPH
D239S mutant enzyme, pH 7.0, 25°C
267
NADPH
D239T mutant enzyme, pH 7.0, 25°C
417
NADPH
D239S/F251Y mutant enzyme, pH 7.0, 25°C
550
NADPH
D239T/F251R mutant enzyme, pH 7.0, 25°C
1.17
O2
-
membrane-bound isoform, pH 7, 37°C
1.37
O2
-
soluble isoform, pH 7, 37°C
1.6.2.2 cytochrome-b5 reductase
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results (
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