1.6.2.2: cytochrome-b5 reductase

This is an abbreviated version!
For detailed information about cytochrome-b5 reductase, go to the full flat file.

Reaction

Synonyms

b5 plus
b5R, b5/b5R, B5R, cb5r, CBR, CBR1, CBR1A, CBR1B, CyB5R, CYB5R2, CYB5R3, Cyb5R4, cyt b5r, cyt b5R protein, cytochrome b5 reductase, cytochrome b5 reductase 2, cytochrome b5 reductase 3, DIA1, diaphorase I, dihydronicotinamide adenine dinucleotide-cytochrome b5 reductase, HPO-19, methemoglobin reductase, NADH cytochrome b5 oxidoreductase, NADH cytochrome B5 reductase, NADH-b5R, NADH-cytochrome b 5 reductase, NADH-cytochrome b5 reductase, NADH-cytochrome b5 reductase 3, NADH-cytochrome-b5 reductase, NADH-cytochrome-b5 reductase 3, NADH-dependent cytochrome b5 reductase, NADH-ferricyanide reductase, NADH-ferricytochrome b5 oxidoreductase, NADH-ferricytochrome reductase, NADH: ferricytochrome b5 oxidoreductase, NADH:cytochrome b5 reductase, NADH:ferricytochrome b5 oxidoreductase, Ncb5or, P34/P32, P35, reduced nicotinamide adeninedinucleotide-cytochrome b5 reductase, reductase, cytochrome b5, T05H4.4

ECTree

     1 Oxidoreductases

         1.6 Acting on NADH or NADPH

             1.6.2 With a heme protein as acceptor

                1.6.2.2 cytochrome-b5 reductase

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Results	in table
4	Activating Compound
9501	AA Sequence
13	Application
1	CAS Registry Number
37	Cloned(Commentary)
143	Cofactor
13	Crystallization (Commentary)
772	Disease/ Diagnostics
3	Expression
36	General Information
13	General Stability
15	IC50 Value
118	Inhibitors
23	kcat/KM [mM/s]
5	Ki Value [mM]
302	KM Value [mM]
111	Localization
9	Metals/Ions
50	Molecular Weight [Da]
75	Natural Substrates/ Products (Substrates)
170	Organism
1	Oxidation Stability
1	Pathway
21	PDB ID
27	pH Optimum
2	pH Range
2	pH Stability
1	pI Value
5	Posttranslational Modification
117	Protein Variants
60	Purification (Commentary)
9	Reaction
3	Reaction Type
167	Reference
1	Renatured (Commentary)
126	Source Tissue
44	Specific Activity [micromol/min/mg]
13	Storage Stability
246	Substrates and Products (Substrate)
41	Subunits
129	Synonyms
1	Systematic Name
8	Temperature Optimum [°C]
7	Temperature Stability [°C]
224	Turnover Number [1/s]

Storage Stability

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Storage Stability on EC 1.6.2.2 - cytochrome-b5 reductase

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STORAGE STABILITY

ORGANISM

UNIPROT

LITERATURE

-20°C, 25 mM phosphate buffer, pH 7.6, 1 mM EDTA, 0.1 mM dithiothreitol, at least a few months

Oryctolagus cuniculus

-

394218

-20°C, pH 7.5-8.1, 0.5 mM EDTA, protein concentration above 0.02 mg/ml

Homo sapiens

-

394225

-20°C, several months, no loss of activity

Oryctolagus cuniculus

-

394227

-70°C, concentrated solution, several months, no loss of activity

Oryctolagus cuniculus

-

394226

-70°C, several months, no loss of activity

Oryctolagus cuniculus

-

394226, 394229

-90°C, oxidized state, 1 month

Tetrahymena pyriformis

-

394219

0°C, at least 1 month

Bos taurus

-

394209

0°C, at least 1 week

Bos taurus

-

394214

4°C, anaerobic conditions, EDTA, dithiothreitol, several months

Phascolopsis gouldii

-

394202

4°C, phosphate buffered saline, pH 7.4, protein concentration 2-3 mg/ml, stable for 3 weeks

Homo sapiens

-

659784

frozen, 50 mM potassium phosphate buffer, pH 7.5, 1 mM EDTA, several weeks

Rattus norvegicus

-

394230

liquid N2

Phascolopsis gouldii

-

394194