1.5.99.4: nicotine dehydrogenase

This is an abbreviated version!
For detailed information about nicotine dehydrogenase, go to the full flat file.

Reaction

Synonyms

6-hydroxy-D-nicotine oxidase, 6-hydroxy-L-nicotine oxidase, D-nicotine oxidase, NctA, NDH, NdhAB, nicotine dehydrogenase, nicotine oxidase, nicotinic acid hydroxylase

ECTree

     1 Oxidoreductases

         1.5 Acting on the CH-NH group of donors

             1.5.99 With unknown physiological acceptors

                1.5.99.4 nicotine dehydrogenase

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Results	in table
1	Activating Compound
15	AA Sequence
1	CAS Registry Number
5	Cloned(Commentary)
23	Cofactor
1	Expression
10	General Information
19	Inhibitors
12	KM Value [mM]
8	Localization
13	Metals/Ions
8	Molecular Weight [Da]
12	Natural Substrates/ Products (Substrates)
40	Organism
5	Pathway
9	pH Optimum
4	Protein Variants
7	Purification (Commentary)
2	Reaction
3	Reaction Type
30	Reference
10	Source Tissue
9	Specific Activity [micromol/min/mg]
4	Storage Stability
48	Substrates and Products (Substrate)
6	Subunits
20	Synonyms
1	Systematic Name
6	Temperature Optimum [°C]
2	Turnover Number [1/s]

Specific Activity

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Specific Activity on EC 1.5.99.4 - nicotine dehydrogenase

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SPECIFIC ACTIVITY [µmol/min/mg]

ORGANISM

UNIPROT

COMMENTARY

LITERATURE

0.000514 - 0.000888

Rattus norvegicus

-

variation caused by pre-treatment with 3-methylcholanthrene, beta-naphthoflavone or phenobarbital

392541

0.0065

Oryctolagus cuniculus

-

respiratory microsomes

392540

0.0222

Oryctolagus cuniculus

-

olfactory microsomes

392540

1096

Pseudomonas putida

-

reduction of 2,6-dichlorophenolindophenol

392542

110

Agrobacterium tumefaciens

-

acceptor pseudoazurin, pH 7, 30°C

763949

22

Agrobacterium tumefaciens

-

acceptor 2,6-dichlorophenolindophenol, pH 7, 30°C

763949

28.3

Pseudarthrobacter oxydans

-

-

392537

29.2

Pseudarthrobacter oxydans

-

-

392538

additional information

Pseudarthrobacter oxydans

-

0.119 units per mg protein, unit is defined as the amount causing a decrease in absorbancy at 578 nm of 0.001 per min

392440