1.5.8.3: sarcosine dehydrogenase

This is an abbreviated version!
For detailed information about sarcosine dehydrogenase, go to the full flat file.

Word Map on EC 1.5.8.3

1.5.8.3

dimethylglycine

sarcosinemia

folate-binding

dmgdh

l-pipecolic

pipox

medicine

Reaction

Synonyms

EC 1.5.99.1, monomethylglycine dehydrogenase, sarcosine N-demethylase, sarcosine:(acceptor) oxidoreductase (demethylating), sarcosine:electron-transfer flavoprotein oxidoreductase (demethylating), SARDH, SDH

ECTree

1 Oxidoreductases

1.5 Acting on the CH-NH group of donors

1.5.8 With a flavin or flavoprotein as acceptor

1.5.8.3 sarcosine dehydrogenase

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Results	in table
2	Activating Compound
187	AA Sequence
2	Application
1	CAS Registry Number
3	Cloned(Commentary)
21	Cofactor
77	Disease/ Diagnostics
2	Expression
3	General Information
1	General Stability
11	Inhibitors
2	Ki Value [mM]
16	KM Value [mM]
14	Localization
4	Metals/Ions
10	Molecular Weight [Da]
27	Natural Substrates/ Products (Substrates)
31	Organism
7	Pathway
6	pH Optimum
2	pH Range
3	pH Stability
1	Protein Variants
6	Purification (Commentary)
1	Reaction
4	Reaction Type
31	Reference
31	Source Tissue
4	Specific Activity [micromol/min/mg]
5	Storage Stability
37	Substrates and Products (Substrate)
2	Subunits
11	Synonyms
1	Systematic Name
2	Temperature Optimum [°C]
1	Temperature Stability [°C]
1	Turnover Number [1/s]

Substrates Products

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Substrates Products on EC 1.5.8.3 - sarcosine dehydrogenase

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N-methyl-L-alanine + acceptor + H2O

L-alanine + formaldehyde + reduced acceptor

3 entries

N-methyl-L-leucine + acceptor + H2O

L-leucine + formaldehyde + reduced acceptor

Pseudomonas putida

acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide

392502

N-methyl-L-valine + acceptor + H2O

L-valine + formaldehyde + reduced acceptor

Pseudomonas putida

acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide

392502

N-methylglycine + acceptor + H2O

glycine + formaldehyde + reduced acceptor

Pseudomonas putida

acceptors: 2,6-dichlorophenolindophenol, phenazine methosulfate, methylene blue, meldola blue, nile blue, potassium ferricyanide

392502

sarcosine + acceptor + H2O

glycine + formaldehyde + reduced acceptor

23 entries

sarcosine + H2O + electron-transfer flavoprotein

glycine + formaldehyde + reduced electron-transfer flavoprotein

Homo sapiens

725421, 742694, 743612, 743719

sarcosine + H2O + phenazine methosulfate

glycine + formaldehyde + reduced phenazine methosulfate

Pseudomonas putida

392502

sarcosine + H2O + potassium ferricyanide

glycine + formaldehyde + potassium ferrocyanide

Pseudomonas putida

392502

sarcosine + oxidized 1-methoxy-5-methylphenazinium methylsulfate

glycine + formaldehyde + reduced 1-methoxy-5-methylphenazinium methylsulfate

Pseudomonas putida

quantification with nitroblue tetrazolium chloride, i.e. NBT or 2,2'-bis(4-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)ditetrazolium chloride, giving NBT-formazan in reaction with reduced 1-methoxy-5-methylphenazinium methylsulfate, overview

655081