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1.5.5.1: electron-transferring-flavoprotein dehydrogenase

This is an abbreviated version!
For detailed information about electron-transferring-flavoprotein dehydrogenase, go to the full flat file.

Word Map on EC 1.5.5.1

Reaction

reduced electron-transferring flavoprotein
+
ubiquinone
=
electron-transferring flavoprotein
 +
ubiquinol

Synonyms

BCAL1468, BCAS0609, electron flavoprotein reductase, electron transfer flavoprotein, electron transfer flavoprotein dehydrogenase, electron transfer flavoprotein Q oxidoreductase, electron transfer flavoprotein ubiquinone oxidoreductase, electron transfer flavoprotein-ubiquinone oxidoreductase, electron transfer flavoprotein: ubiquinone oxidoreductase, electron transfer flavoprotein:ubiquinone oxidoreductase, electron-transfer flavoprotein-2,3-dimethoxy-5-methyl-1,4-benzoquinone oxidoreductase, electron-transfer flavoprotein-ubiquinone oxidoreductase, electron-transfer flavoprotein:ubiquinone oxidoreductase, electron-transferring flavoprotein dehydrogenase, Electron-transferring-flavoprotein dehydrogenase, ETF dehydrogenase, ETF-QO, ETF-ubiquinone oxidoreductase, ETF:QO, ETF:quinone oxidoreductase, ETF:ubiquinone oxidoreductase, EtfA2, ETFDH, ETFDH1, ETFDH2, ETFQO, let-721

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.5 With a quinone or similar compound as acceptor
                1.5.5.1 electron-transferring-flavoprotein dehydrogenase

Cloned

Cloned on EC 1.5.5.1 - electron-transferring-flavoprotein dehydrogenase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cloning of a putative ETF-QO orthologue, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL
cloning of the electron-transferring flavoprotein (ETF) alpha and beta subunit encoding genes ETFA and ETFB and enzyme ETFDH encoding gene ETFDH from Magnaporthe oryzae, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant expression of GFP-tagged genes ETFA, ETFB, and ETFDH in Magnaporthe oryzae cells
deletion mutant enzyme lacking 74 amino acids among them C561, and a C561A mutant
-
expressed from a baculovirus vector, expressed in Sf9 cells
-
expressed in Escherichia coli C43
-
expressed in Escherichia coli C43 cells
-
expressed in Escherichia coli strain C43
-
expression in insect Sf9 cells via transfection using the baculovirus system
-
expression in Saccharomyces cerevisiae, the enzyme is synthesized as a 67000 Da precursor which is targeted to mitochondria and processed in a single step to a 64000 Da mature form
-
expression in Sf9 insect cells
-
fixABCX operon, DNA and sequence determination and analysis, analysis of promotor activation and regulation
-
gene CG12140, mapping and cloning of ETF:QO alleles, expression of the two subunits ETFA and ETFB in Escherichia coli strain BL21
-
gene ETF:QO, DNA and amino acid sequence determination and analysis, localization on chromosome 4q33, 13 exons
recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL
RT-PCR enzyme expression analysis
-