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1.5.3.17: non-specific polyamine oxidase

This is an abbreviated version!
For detailed information about non-specific polyamine oxidase, go to the full flat file.

Word Map on EC 1.5.3.17

Reaction

N1-acetylspermidine
+
O2
+
H2O
=
putrescine
+
3-acetamidopropanal
+
H2O2

Synonyms

AtPAO2, AtPAO3, AtPAO4, AtPAO5, Bjpao1, Bjpao2, EC 1.5.3.11, Fms1, LC036642, OsPAO1, PAO, PAO1, PAO2, PAO3, PAO5, polyamine oxidase 1, SelPAO5, slr5093, T-Spm oxidase, thermospermine oxidase

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.3 With oxygen as acceptor
                1.5.3.17 non-specific polyamine oxidase

Purification

Purification on EC 1.5.3.17 - non-specific polyamine oxidase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
native enzyme partially from leaves by ammonium sulfate fractionation
-
recombinant AtPAO3 fused to the maltose-binding protein from Escherichia coli
recombinant His-tagged wild-type and mutant enzymes by nickel affinity chhromatography
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography
recombinant maltose-binding protein fusion enzyme from Escherichia coli by amylose affinity chromatography
recombinant wild-type and mutant enzymes