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1.5.1.8: saccharopine dehydrogenase (NADP+, L-lysine-forming)

This is an abbreviated version!
For detailed information about saccharopine dehydrogenase (NADP+, L-lysine-forming), go to the full flat file.

Word Map on EC 1.5.1.8

Reaction

N6-(L-1,3-dicarboxypropyl)-L-lysine
+
NADP+
 +
H2O
=
L-lysine
 +
2-oxoglutarate
 +
NADPH
 +
H+

Synonyms

AasS, dehydrogenase, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming), L-lysine-alpha-ketoglutarate reductase, LKR, LKR/SDH, LOR, LOR-SDH, lysine 2-oxoglutarate reductase, lysine 2-oxoglutarate reductase-saccharopine dehydrogenase, lysine alpha-ketoglutarate reductase, lysine ketoglutarate reductase, lysine ketoglutarate/reductase saccharopine dehydrogenase, lysine-2-oxoglutarate reductase, lysine-alpha-ketoglutarate reductase, lysine-ketoglutarate reductase, lysine-ketoglutarate reductase/saccharopine dehydrogenase, lysine-ketoglutaric reductase, lysine:alpha-ketoglutarate:TPNH oxidoreductase (epsilon-N-[glutaryl-2]-L-lysine forming), nllkr/sdh, saccharopine (nicotinamide adenine dinucleotide phosphate, lysine-forming) dehydrogenase, saccharopine dehydrogenase, saccharopine dehydrogenase (NADP, lysine-forming), saccharopine dehydrogenase (nicotinamide adenine dinucleotide phosphate, lysine-forming)

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.8 saccharopine dehydrogenase (NADP+, L-lysine-forming)

Purification

Purification on EC 1.5.1.8 - saccharopine dehydrogenase (NADP+, L-lysine-forming)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
1630.5fold purification of a bifunctional protein containing lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
300fold purification, enzyme clearly separated from saccharopine dehydrogenase EC 1.5.1.9
-
38fold purification
-
44.6fold partial purification from liver
-
450fold purification, no separation of lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9
-
500fold purification of a single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
9.6fold partial purification from liver
-
native enzyme partially through ammonium sulfate fractionation and desalting gel filtration
-
partial purification
-
partial purification of a recombinant bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities and of the monofunctional lysine-2-oxoglutarate reductase domain
-
partial purification of a single bifunctional protein with lysine-2-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
partial purification of bifunctional enzyme with lysine-oxoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities
-
separation of lysine-ketoglutarate reductase and saccharopine dehydrogenase activities by limited proteolysis with elastase, chymotrypsin, and papain
-
single protein with lysine-ketoglutarate reductase EC 1.5.1.8 and saccharopine dehydrogenase EC 1.5.1.9 activities: aminoadipic semialdehyde synthase
-