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1.5.1.3: dihydrofolate reductase

This is an abbreviated version!
For detailed information about dihydrofolate reductase, go to the full flat file.

Word Map on EC 1.5.1.3

Reaction

5,6,7,8-tetrahydrofolate
+
NADP+
=
7,8-dihydrofolate
 +
NADPH
 +
H+

Synonyms

5,6,7,8-tetrahydrofolate: NADP+ oxidoreductase, 7,8-dihydrofolate reductase, At2g16370, At4g34570, bifunctional dihydrofolate reductase-thymidylate synthase, bifunctional TS-DHFR, BmDHFR, dehydrogenase, tetrahydrofolate, DFR-TS, DFR1, DfrA, DfrB, DHFR, DHFR type IIIC, DHFR-TS, DHFR-TS1, DHFR-TS2, DHFR2, DHFRL1, DHFRLS, dihydrofolate reductase, dihydrofolate reductase-like, dihydrofolate reductase-thymidylate synthase, dihydrofolate reductase:thymidylate synthase, dihydrofolic acid reductase, dihydrofolic reductase, EC 1.5.1.4, ecDHFR, folA, folA3, folic acid reductase, folic reductase, FolM, hDHFR, hDHFR-1, hDHFR-2, HjDHFR, hvDHFR1, hvDHFR2, LAU_0427, LBRM_06_0830, mDHFR, mjDHFR, More, myDHFR, NADPH-dihydrofolate reductase, pcDHFR, PKNH_0509600, ppDHFR, pteridine reductase, pteridine reductase:dihydrofolate reductase, PTR2, R-plasmid-encoded dihydrofolate reductase, R67 DHFR, R67 dihydrofolate reductase, reductase, dihydrofolate, S3DHFR, Smdhfr, Smp 175230, spDHFR, svDHFR, tcptr1, tetrahydrofolate dehydrogenase, THY-1, THY-2, thymidylate synthase-dihydrofolate reductase, thymidylate synthetase-dihydrofolate reductase, Trimethoprim resistance protein, TS-DHFR, WUBG_00817

ECTree

     1 Oxidoreductases
         1.5 Acting on the CH-NH group of donors
             1.5.1 With NAD+ or NADP+ as acceptor
                1.5.1.3 dihydrofolate reductase

Purification

Purification on EC 1.5.1.3 - dihydrofolate reductase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
2 forms
-
2 forms: mutant-type and wild type enzyme in a single organism
-
affinity chromatography
amethopterin-resistant strain
-
baby hamster kidney cells, 2 forms: I, II
-
bifunctional enzyme: pteridine reductase-dihydrofolate reductase
-
cultured mammalian cells, overview
-
dihydrofolate reductase-thymidylate synthase EC 2.1.1.45
-
enzyme complex with dihydrofolate reductase activity, thymidylate synthase activity and three other polypeptides of unknown function
-
Escherichia coli B, R-plasmid enzyme
-
fragments, partial
-
green algae, thymidylate synthase and dihydrofolate reductase cannot be dissociated from each other
-
HiPrep 16/10 SP-XL column chromatography
-
isozyme hDHFR-2 from hdrB gene overexpressed in Escherichia coli
-
isozyme type II
-
isozymes I and II
-
large quantities of recombinant wild-type enzyme from Escherichia coli, purification of mutants L30K, A31K and double mutant L30K/A31K recombinant from Escherichia coli
-
methotrexate-affinity chromatography
methotrexate-resistant mutant: large-scale
-
methotrexate-resistant strain
-
methotrexate-sensitive and methotrexate resistant cells
-
methotrexate-Sepharose column chromatography, SP-Sepharose column chromatography, and Sephacryl S-200 and gel filtration
-
native enzyme partially by purification of mitochondria
Ni-NTA column chromatography
Ni-NTA resin column chromatography
-
nickel affinity column chromatography
overview
phage type 179, R-plasmid enzyme, isozyme type III
-
pteroyllysine-agarose
-
purification by amethopterin-agarose affinity chromatography
-
purification by methotrexate-affinity chromatography
-
recombinant bifunctional protein from Escherichia coli
-
recombinant C-terminally StrepII-tagged DHFR from Escherichia coli strain BL21(DE3)pLysS by affinity chromatography and tag cleavage with thrombin, followed by another step of affinity chromatography and gell filtration
recombinant enzyme
recombinant enzyme from Escherichia coli
recombinant enzyme from Escherichia coli by methotrexate affinity chromatography
-
recombinant from Escherichia coli
recombinant GST-tagged enzyme from Escherichia coli by glutathione affinity chromatography, the tag is cleaved off
recombinant His-tagged enzyme by metal affinity chromatography
recombinant His-tagged enzyme from Escherichia coli
recombinant His-tagged enzyme from Escherichia coli by two steps of nickel affinity chromatography with Ulp1 protease cleavage after the first step
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and dialysis
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration
recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration to homogeneity
recombinant His-tagged enzyme from Escherichia coli strain M15(pREP4) by nickel affinity chromatography
recombinant His-tagged mutant V77A/I130M/I138V from Escherichia colii strain M15 by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzmyes from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli Rosetta Gami B (DE3) by nickel affinity chromatography, and tag cleavage through the SUMO protease Ulp1, followed by another step of nickel affinity chromatography to remove the tag
recombinant His-tagged wild-type enzyme from Escherichia coli Rosetta Gami B (DE3) by nickel affinity chromatography, and tag cleavage through the SUMO protease Ulp1, followed by another step of nickel affinity chromatography to remove the tag
recombinant His-tagged wild-type mDHFR and modified mDHFR-43pEthF enzymes from Escherichia coli strain XL1-Blue by nickel affinity chromatography
recombinant His6-tagged enzyme from Escherichia coli by nickel affinity chromatography, and gel filtration
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
-
recombinant protein, using Ni-NTA resin and imidazole concentration in the wash buffer is changed from 25 mM to 100 mM
recombinant purified from Escherichia coli
-
recombinant Trypanosoma brucei Tsf-DHFR-TS from Escherichia coli strain BL21 Star (DE3) by methotrexate affinity chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (AI) by adsorption chromatography and gel filtration
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21 (DE3)
recombinant wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by ammonium sulfate fractionation, methotrexate affinity chromatography, dialysis and anion exchange chromatography
refolding and purification of wild-type and mutant enzymes from inclusion bodies in Escherichia coli
-
single-step purification on pteridine matrices
-
strain MB 3746, MB 3747
-
trimethoprim-resistant
-
using affinity chromatography
using gel filtration
using Ni-NTA chromatography
via methotrexate-amino-ethyl starch
-
Walker 256 carcinosarcoma cells
-
wild type and methotrexate-resistant cells
-
wild-type and mutants from high expression vector pCOCK, large scale purification
-
wild-type enzyme readily aggregates at 2 mg/ml
wild-type, seleno-containing and telluro-containing enzymes