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0.003
10-formyl-dihydrofolate
pH 7.5, temperature not specified in the publication
0.0034
6-Hydroxymethylpterin
-
-
0.00004 - 21.7
7,8-dihydrofolate
0.03 - 0.3
8-Methylpterin
0.00063 - 0.238
dihydrofolate
0.0009
dihydropteroate
-
-
0.0035
L-threo-neopterin
-
-
0.123
NADP+
pH 7.8, 25°C, recombinant enzyme
additional information
additional information
-
0.00004
7,8-dihydrofolate
mutant Q134K, pH 8.5, 25°C
0.000075
7,8-dihydrofolate
-
wild-type, pH 7.6, 23°C
0.000075
7,8-dihydrofolate
wild-type, pH 7.6, 23°C
0.00015
7,8-dihydrofolate
-
with NADPH
0.00018
7,8-dihydrofolate
pH 7.4, 37°C, mutant N64S
0.00022
7,8-dihydrofolate
pH 6, temperature not specified in the publication, isolate PKNY146CSP
0.0003
7,8-dihydrofolate
-
recombinant enzyme
0.0003
7,8-dihydrofolate
-
pH 7.4, 37°C
0.00039
7,8-dihydrofolate
-
recombinant enzyme
0.0004
7,8-dihydrofolate
-
-
0.0004
7,8-dihydrofolate
recombinant mutant Q35K/N64F, pH 7.4, 37°C
0.0004
7,8-dihydrofolate
recombinant mutant Q35S/N64S, pH 7.4, 37°C
0.0004
7,8-dihydrofolate
chimera with Haemophilus influenzae enzyme, pH 7, 25°C
0.00044
7,8-dihydrofolate
-
-
0.00047
7,8-dihydrofolate
-
-
0.00048
7,8-dihydrofolate
-
recombinant enzyme
0.00049
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35K/N64F
0.0005
7,8-dihydrofolate
recombinant mutant Q35K, pH 7.4, 37°C
0.0006
7,8-dihydrofolate
-
recombinant bifunctional enzyme
0.00061
7,8-dihydrofolate
-
mutant M42I, pH 7.0, 25°C
0.00068
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32F
0.00069
7,8-dihydrofolate
mutant F31R/Q35E, pH 7.6, 23°C
0.0007
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
0.0007
7,8-dihydrofolate
pH 7.0, 30°C, wild-type
0.0007
7,8-dihydrofolate
-
mutant enzyme R44H, at 15°C, pH not specified in the publication
0.0007
7,8-dihydrofolate
recombinant mutant Q35K/N64S, pH 7.4, 37°C
0.0007
7,8-dihydrofolate
recombinant mutant Q35S, pH 7.4, 37°C
0.0007
7,8-dihydrofolate
recombinant mutant Q35S/N64F, pH 7.4, 37°C
0.0007
7,8-dihydrofolate
-
pH 7, 20°C, wild-type enzyme
0.0007
7,8-dihydrofolate
chimera with Listeria grayi enzyme, pH 7, 25°C
0.00074
7,8-dihydrofolate
-
-
0.00076
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35K
0.00078
7,8-dihydrofolate
pH 7.0, 30°C, mutant V76A
0.0008
7,8-dihydrofolate
wild-type, pH 7, 25°C
0.0008
7,8-dihydrofolate
deletion mutant DELTAAla145, at pH 7.0 and 25°C
0.0008
7,8-dihydrofolate
-
mutant enzyme R44V, at 15°C, pH not specified in the publication
0.0008
7,8-dihydrofolate
recombinant mutant N64F, pH 7.4, 37°C
0.00081
7,8-dihydrofolate
pH 7.4, 37°C, mutant N64F
0.00082
7,8-dihydrofolate
-
mutant M42T, pH 7.0, 25°C
0.00083
7,8-dihydrofolate
-
mutant M42P, pH 7.0, 25°C
0.0009
7,8-dihydrofolate
recombinant mutant N64S, pH 7.4, 37°C
0.00091
7,8-dihydrofolate
-
mutant M42V, pH 7.0, 25°C
0.00096
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32A
0.00097
7,8-dihydrofolate
-
wild-type enzyme from plasmid
0.001
7,8-dihydrofolate
-
mutant enzyme R44Y, at 15°C, pH not specified in the publication
0.0011
7,8-dihydrofolate
-
-
0.0011
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35S
0.0011
7,8-dihydrofolate
-
mutant enzyme R44C, at 15°C, pH not specified in the publication
0.00112
7,8-dihydrofolate
-
mutant M42L, pH 7.0, 25°C
0.00118
7,8-dihydrofolate
-
mutant M42G, pH 7.0, 25°C
0.00119
7,8-dihydrofolate
wild-type, pH 8.5, 25°C
0.0012
7,8-dihydrofolate
mutant C85A/C152S, 15°C, pH 7.0
0.0012
7,8-dihydrofolate
mutant M1P/C85A/C152S, 15°C, pH 7.0
0.0012
7,8-dihydrofolate
wild-type, 15°C, pH 7.0
0.0012
7,8-dihydrofolate
-
wild-type, 15°C, pH 7.0
0.00123
7,8-dihydrofolate
-
mutant L92M from plasmid
0.0013
7,8-dihydrofolate
-
wild-type enzyme
0.0013
7,8-dihydrofolate
Halalkalibacterium halodurans
-
wild-type, pH 7.5, 37°C
0.0013
7,8-dihydrofolate
-
wild-type, pH 7.0, 25°C
0.0013
7,8-dihydrofolate
mutant M1A/C85A/C152S, 15°C, pH 7.0
0.0013
7,8-dihydrofolate
-
mutant M42A, pH 7.0, 25°C
0.0013
7,8-dihydrofolate
wild type enzyme, at pH 7.0 and 25°C
0.0014
7,8-dihydrofolate
-
mutant enzyme R44L, at 15°C, pH not specified in the publication
0.0015
7,8-dihydrofolate
-
-
0.0015
7,8-dihydrofolate
mutant F31P, pH 7.6, 23°C
0.0015
7,8-dihydrofolate
-
mutant enzyme R44G, at 15°C, pH not specified in the publication
0.0015
7,8-dihydrofolate
chimera with Saccharomyces cerevisiae enzyme, pH 7, 25°C
0.0016
7,8-dihydrofolate
-
mutant R53M enzyme
0.0016
7,8-dihydrofolate
25°C, recombinant GST-tagged enzyme
0.0016
7,8-dihydrofolate
pH 7.4, 37°C, mutant Q35S/N64S
0.0016
7,8-dihydrofolate
-
mutant enzyme R44A, at 15°C, pH not specified in the publication
0.0016
7,8-dihydrofolate
-
wild type enzyme, at 15°C, pH not specified in the publication
0.0016
7,8-dihydrofolate
-
with NADH as cosubstrate, at 25°C and 100 mM HEPES, pH 7.5
0.00167
7,8-dihydrofolate
-
double mutant L16M/L20M from plasmid
0.0017
7,8-dihydrofolate
mutant F31G/Q35N, pH 7.6, 23°C
0.0017
7,8-dihydrofolate
mutant F31R/F34T/Q35S, pH 7.6, 23°C
0.00171
7,8-dihydrofolate
-
mutant L42M from plasmid
0.00178
7,8-dihydrofolate
-
mutant M42Y, pH 7.0, 25°C
0.0018
7,8-dihydrofolate
-
25°C, pH 7.4
0.0019
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31E
0.0019
7,8-dihydrofolate
-
mutant enzyme R44E, at 15°C, pH not specified in the publication
0.00193
7,8-dihydrofolate
-
mutant M42H, pH 7.0, 25°C
0.00194
7,8-dihydrofolate
-
mutant M42C, pH 7.0, 25°C
0.002
7,8-dihydrofolate
-
-
0.002
7,8-dihydrofolate
-
His-tagged human-derived enzyme, pH 7.65
0.002
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
0.002
7,8-dihydrofolate
mutant F31R/F34T/Q35R, pH 7.6, 23°C
0.0021
7,8-dihydrofolate
-
pH 6.0, 25°C
0.0021
7,8-dihydrofolate
mutant F31P/Q35H, pH 7.6, 23°C
0.0021
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31N
0.0021
7,8-dihydrofolate
-
mutant enzyme R44I, at 15°C, pH not specified in the publication
0.0022
7,8-dihydrofolate
mutant F31R/F34A/Q35N, pH 7.6, 23°C
0.00222
7,8-dihydrofolate
-
mutant L16M from plasmid
0.00227
7,8-dihydrofolate
-
mutant M42Q, pH 7.0, 25°C
0.00227
7,8-dihydrofolate
-
mutant M42S, pH 7.0, 25°C
0.0023
7,8-dihydrofolate
Tequatrovirus T4
-
-
0.0023
7,8-dihydrofolate
-
recombinant enzyme, pH 7.0, 0.1 M imidazole
0.0023
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31Q
0.0023
7,8-dihydrofolate
-
mutant enzyme R44M, at 15°C, pH not specified in the publication
0.0024
7,8-dihydrofolate
-
native enzyme
0.0024
7,8-dihydrofolate
-
mutant enzyme R44Q, at 15°C, pH not specified in the publication
0.0024
7,8-dihydrofolate
recombinant mutant S37K/S69N, pH 7.4, 37°C
0.0025
7,8-dihydrofolate
mutant M1S/C85A/C152S, 15°C, pH 7.0
0.0025
7,8-dihydrofolate
-
mutant enzyme R44K, at 15°C, pH not specified in the publication
0.0025
7,8-dihydrofolate
-
mutant enzyme R44N, at 15°C, pH not specified in the publication
0.0025
7,8-dihydrofolate
-
mutant enzyme R44T, at 15°C, pH not specified in the publication
0.00268
7,8-dihydrofolate
-
-
0.0027
7,8-dihydrofolate
-
recombinant from E. coli
0.0027
7,8-dihydrofolate
-
pH 7.4, 37°C
0.0027
7,8-dihydrofolate
pH 7.4, 37°C, wild-type enzyme
0.0027
7,8-dihydrofolate
mutant F31P/Q35E, pH 7.6, 23°C
0.0027
7,8-dihydrofolate
-
mutant enzyme R44F, at 15°C, pH not specified in the publication
0.0027
7,8-dihydrofolate
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0028
7,8-dihydrofolate
-
pH 7.3, 37°C
0.0028
7,8-dihydrofolate
-
pH 7.4, 37°C, wild-type enzyme
0.00289
7,8-dihydrofolate
-
mutant L20M from plasmid
0.0029
7,8-dihydrofolate
Halalkalibacterium halodurans
-
His-tagged recombinant enzyme, pH 7.5, 37°C
0.0029
7,8-dihydrofolate
Halalkalibacterium halodurans
-
His-tagged enzyme, pH 7.5, 37°C
0.0029
7,8-dihydrofolate
-
mutant enzyme R44P, at 15°C, pH not specified in the publication
0.003
7,8-dihydrofolate
-
-
0.003
7,8-dihydrofolate
-
-
0.003
7,8-dihydrofolate
-
wild-type enzyme
0.003
7,8-dihydrofolate
-
strain PC 174
0.003
7,8-dihydrofolate
mutant M1A/M16F/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.003
7,8-dihydrofolate
-
mutant enzyme R44D, at 15°C, pH not specified in the publication
0.003
7,8-dihydrofolate
recombinant mutant S37Q/S69F, pH 7.4, 37°C
0.0031
7,8-dihydrofolate
-
mutant enzyme R44S, at 15°C, pH not specified in the publication
0.0032
7,8-dihydrofolate
recombinant mutant S37K/S69F, pH 7.4, 37°C
0.0033
7,8-dihydrofolate
mutant F31S/Q35E, pH 7.6, 23°C
0.0034
7,8-dihydrofolate
recombinant mutant S69N, pH 7.4, 37°C
0.0035
7,8-dihydrofolate
recombinant wild-type enzyme, pH 7.4, 37°C
0.0035
7,8-dihydrofolate
recombinant mutant S37Q, pH 7.4, 37°C
0.00369
7,8-dihydrofolate
-
triple mutant L16M/L20M/L42M from plasmid
0.0037
7,8-dihydrofolate
pH 6, 25°C
0.0037
7,8-dihydrofolate
-
pH 7.0, 60°C
0.0037
7,8-dihydrofolate
-
mutant enzyme R44W, at 15°C, pH not specified in the publication
0.0038
7,8-dihydrofolate
mutant M1A/M16S/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.0038
7,8-dihydrofolate
recombinant mutant S37Q/S69N, pH 7.4, 37°C
0.00385
7,8-dihydrofolate
-
wild type enzyme, in 50 mM TES, pH 7.0, at 37°C
0.004
7,8-dihydrofolate
-
-
0.004
7,8-dihydrofolate
-
strain PC 178
0.0042
7,8-dihydrofolate
wild-type, pH 5.0, 25°C
0.0043
7,8-dihydrofolate
mutant F31A/F34V/Q35H, pH 7.6, 23°C
0.0045
7,8-dihydrofolate
-
pH 7.3, 37°C
0.0047
7,8-dihydrofolate
25°C, recombinant detagged enzyme
0.0048
7,8-dihydrofolate
-
-
0.0048
7,8-dihydrofolate
deletion mutant DELTAGly67/DELTAAla145, at pH 7.0 and 25°C
0.0048
7,8-dihydrofolate
recombinant mutant S69F, pH 7.4, 37°C
0.0049
7,8-dihydrofolate
-
pH 7.4, 37°C, wild-type enzyme
0.0049
7,8-dihydrofolate
mutant Y35G/F37L, pH 5.0, 25°C
0.005
7,8-dihydrofolate
-
mutant N51I/C59R/N108T/I164L, pH 7.0
0.0051
7,8-dihydrofolate
-
wild-type, 25°C, pH 7.0
0.0051
7,8-dihydrofolate
pH 7.0, 30°C, mutant L32D
0.0051
7,8-dihydrofolate
deletion mutant DELTAGly67, at pH 7.0 and 25°C
0.0051
7,8-dihydrofolate
-
pH 6.0, 25°C, presence of 200 mM NaCl
0.0052
7,8-dihydrofolate
-
mutant N51I/C59R/N108S/I164L, pH 7.0
0.0053
7,8-dihydrofolate
mutant K27E, pH 5.0, 25°C
0.0055
7,8-dihydrofolate
mutant Y35L/F37R, pH 5.0, 25°C
0.0055
7,8-dihydrofolate
pH 7.5, temperature not specified in the publication
0.0056
7,8-dihydrofolate
-
recombinant rat-derived enzyme, pH 7.65
0.0056
7,8-dihydrofolate
deletion mutant DELTAGly67/DELTAGly121, at pH 7.0 and 25°C
0.00592
7,8-dihydrofolate
mutant L30Q, pH 8.5, 25°C
0.00597
7,8-dihydrofolate
-
mutant M42E, pH 7.0, 25°C
0.0062
7,8-dihydrofolate
-
-
0.0062
7,8-dihydrofolate
mutant linear ANLYF-G6, 15°C, pH 7.0
0.0063
7,8-dihydrofolate
chimera with Streptococcus dysgalactiae enzyme, pH 7, 25°C
0.0066
7,8-dihydrofolate
recombinant mutant F69N, pH 7.4, 37°C
0.007
7,8-dihydrofolate
-
mutant V115C, pH 7.6, 23°C
0.0071
7,8-dihydrofolate
recombinant mutant F69S, pH 7.4, 37°C
0.0073
7,8-dihydrofolate
deletion mutant DELTAGly121/DELTAAla145, at pH 7.0 and 25°C
0.0076
7,8-dihydrofolate
mutant Y35Q/F37R, pH 5.0, 25°C
0.0077
7,8-dihydrofolate
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.008
7,8-dihydrofolate
-
pH 8.0, 25°C, presence of 500 mM NaCl
0.0081
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/K96N, pH 7.0
0.0081
7,8-dihydrofolate
-
pH 6.0, 25°C, presence of 500 mM NaCl
0.0083
7,8-dihydrofolate
mutant M1A/M16N/M20L/M42Y/M92F/C85A/C152S, 15°C, pH 7.0
0.0083
7,8-dihydrofolate
-
mutant I51N/C59R/N108S/I164L, pH 7.0
0.0083
7,8-dihydrofolate
mutant M1A/M16N/M20L/M42Y/C85A/M92F/C152S, 15°C, pH 7.0
0.0085
7,8-dihydrofolate
-
pH 8.0, 25°C
0.0085
7,8-dihydrofolate
-
pH 7.4, 37°C, mutant K37Q
0.0086
7,8-dihydrofolate
-
-
0.0086
7,8-dihydrofolate
mutant Y35L/F37T, pH 5.0, 25°C
0.0086
7,8-dihydrofolate
mutant linear ANLYF-G8, 15°C, pH 7.0
0.0089
7,8-dihydrofolate
-
pH 8.0, 25°C, presence of 200 mM NaCl
0.0095
7,8-dihydrofolate
-
pH 6.0
0.0096
7,8-dihydrofolate
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
0.0097
7,8-dihydrofolate
recombinant mutant K37S/F69N, pH 7.4, 37°C
0.01
7,8-dihydrofolate
-
altered enzyme
0.0101
7,8-dihydrofolate
recombinant mutant K37S, pH 7.4, 37°C
0.0106
7,8-dihydrofolate
-
native rat-derived enzyme, pH 7.65
0.0107
7,8-dihydrofolate
recombinant mutant K37Q/F69N, pH 7.4, 37°C
0.0108
7,8-dihydrofolate
deletion mutant DELTAGly121, at pH 7.0 and 25°C
0.0115
7,8-dihydrofolate
mutant circular ANLYF-G6, 15°C, pH 7.0
0.0116
7,8-dihydrofolate
recombinant mutant K37S/F69S, pH 7.4, 37°C
0.013
7,8-dihydrofolate
-
-
0.013
7,8-dihydrofolate
-
recombinant wild-type enzyme
0.013
7,8-dihydrofolate
mutant circular ANLYF-G7, 15°C, pH 7.0
0.0133
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L, pH 7.0
0.014
7,8-dihydrofolate
-
recombinant mutants S108T and S108A
0.014
7,8-dihydrofolate
pH 6.8, 23°C
0.014
7,8-dihydrofolate
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
0.014
7,8-dihydrofolate
mutant circular ANLYF-G8, 15°C, pH 7.0
0.01444
7,8-dihydrofolate
mutant enzyme Y102F, at pH 7.0 and 25°C
0.0145
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/D187A, pH 7.0
0.01465
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L/I150V/N182I/N201D, pH 7.0
0.0147
7,8-dihydrofolate
-
pH 6, 25°C
0.0147
7,8-dihydrofolate
-
above, pH 6.0, 25°C recombinant His-tagged enzyme
0.015
7,8-dihydrofolate
-
mutant V115A, pH 7.6, 23°C
0.01625
7,8-dihydrofolate
-
mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C
0.017
7,8-dihydrofolate
-
-
0.0178
7,8-dihydrofolate
25°C, pH 7.0, pressure 0.1 MPa
0.018
7,8-dihydrofolate
pH 6.8, 23°C
0.0185
7,8-dihydrofolate
recombinant mutant K37Q/F69S, pH 7.4, 37°C
0.0195
7,8-dihydrofolate
25°C, pH 7.0, pressure 200 MPa
0.02 - 0.025
7,8-dihydrofolate
-
recombinant mutants S108G, S108N and S108C
0.0207
7,8-dihydrofolate
-
mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C
0.0209
7,8-dihydrofolate
-
mutant N51I/C59R/S108N, pH 8.0, 25°C
0.022
7,8-dihydrofolate
-
-
0.0231
7,8-dihydrofolate
-
mutant W48Y, 25°C, pH 7.0
0.0264
7,8-dihydrofolate
pH 7.8, 25°C, recombinant enzyme
0.03051
7,8-dihydrofolate
-
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C
0.0324
7,8-dihydrofolate
-
mutant W48Y/N188Y, 25°C, pH 7.0
0.0337
7,8-dihydrofolate
wild type enzyme, at pH 7.0 and 25°C
0.035
7,8-dihydrofolate
-
-
0.036
7,8-dihydrofolate
-
-
0.037
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31A
0.03825
7,8-dihydrofolate
at pH 7.0 and 22°C
0.0444
7,8-dihydrofolate
-
wild-type, pH 8.0, 25°C
0.045
7,8-dihydrofolate
-
mutant M42W, pH 7.0, 25°C
0.05
7,8-dihydrofolate
-
recombinant mutant S108Q
0.0502
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAGly67, at pH 7.0 and 25°C
0.0512
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAGly121, at pH 7.0 and 25°C
0.0556
7,8-dihydrofolate
pH 6.0, 28°C, recombinant enzyme
0.058
7,8-dihydrofolate
chimera with Plasmodium falciparum enzyme, pH 7, 25°C
0.067
7,8-dihydrofolate
chimera with Homo sapiens enzyme, pH 7, 25°C
0.0777
7,8-dihydrofolate
deletion mutant DELTAArg52/DELTAAla145, at pH 7.0 and 25°C
0.08
7,8-dihydrofolate
-
lowest Km value in 3.0 M KCl, recombinant wild-type enzyme
0.097
7,8-dihydrofolate
-
mutant enzyme
0.107
7,8-dihydrofolate
-
wild-type enzyme
0.1161
7,8-dihydrofolate
deletion mutant DELTAArg52, at pH 7.0 and 25°C
0.198
7,8-dihydrofolate
pH 7.0, 30°C, mutant D31L
0.2301
7,8-dihydrofolate
mutant enzyme F96I, at pH 7.0 and 25°C
16.4
7,8-dihydrofolate
pH 7.0, 20°C
19.8
7,8-dihydrofolate
pH 7.0, 30°C
21.7
7,8-dihydrofolate
pH 7.0, 5°C
0.03
8-Methylpterin
-
pH 5.0
0.03
8-Methylpterin
-
pH 5.0
0.13
8-Methylpterin
-
pH 5.0
0.2
8-Methylpterin
-
pH 7.4
0.2
8-Methylpterin
-
pH 7.4
0.3
8-Methylpterin
-
above pH 7.4
0.00063
dihydrofolate
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C, value below
0.001
dihydrofolate
-
pH 7.0, 25°C, under atmospheric pressure
0.0018
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
0.0019
dihydrofolate
pH 7.5, 25°C
0.0019
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
0.004
dihydrofolate
wild-type, pH 7.0, 25°C
0.0062
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
0.238
dihydrofolate
pH 7.0, 25°C, under atmospheric pressure
0.0041
folate
-
bifunctional enzyme DHFR-thymidylate synthase
0.268
NADH
-
-
0.00043
NADPH
-
25°C, pH 7.6
0.00065
NADPH
pH 7.0, 30°C, mutant D31Q
0.00068
NADPH
pH 7.0, 30°C, mutant D31A
0.00075
NADPH
mutant H26Y/Q60K/A77V/V78A/Q81H/Q91S/L100V/E133A/A149T, pH 7.0, 25°C
0.00077
NADPH
pH 7.0, 30°C, mutant D31N
0.00084
NADPH
pH 7.0, 30°C, mutant L32D
0.0009
NADPH
-
wild-type enzyme
0.0009
NADPH
-
recombinant bifunctional enzyme
0.001
NADPH
-
for native and recombinant enzymes, monophasic
0.001
NADPH
-
for native and recombinant enzymes, monophasic
0.001
NADPH
-
Km less than 0.001 mM, at 25°C and 100 mM HEPES, pH 7.5
0.0012
NADPH
-
mutant R53M enzyme
0.0013
NADPH
pH 7.0, 30°C, mutant L32F
0.00133
NADPH
-
recombinant enzyme
0.0014
NADPH
pH 7.0, 30°C, mutant D31L
0.0014
NADPH
pH 7.0, 30°C, mutant L32A
0.0015
NADPH
pH 7.0, 30°C, mutant V76A
0.0015
NADPH
pH 7.0, 30°C, wild-type
0.0019
NADPH
-
pH 7.0, 25°C, under atmospheric pressure
0.002
NADPH
pH 7.0, 30°C, mutant D31E
0.0021
NADPH
-
with dihydropteroate
0.0022
NADPH
-
altered enzyme
0.00228
NADPH
-
double mutant L16M/L20M from plasmid
0.0023
NADPH
pH 6, temperature not specified in the publication, isolate PKNY146CSP
0.00232
NADPH
-
mutant L16M from plasmid
0.00242
NADPH
-
pH 7.4, 37°C
0.0025 - 0.0032
NADPH
-
-
0.00252
NADPH
-
wild-type enzyme from plasmid
0.0027
NADPH
-
mutant L92M from plasmid
0.0027
NADPH
-
wild-type, 25°C, pH 7.0
0.0027
NADPH
-
pH 6.0, 25°C, presence of 200 mM NaCl
0.003
NADPH
-
mutant L42M from plasmid
0.003
NADPH
-
recombinant enzyme, pH 7.0, 0.1 M imidazole
0.0034
NADPH
pH 7.0, 25°C, under atmospheric pressure
0.00345
NADPH
-
mutant W48Y/N188Y, 25°C, pH 7.0
0.0036
NADPH
-
pH 8.0, 25°C, presence of 500 mM NaCl
0.0038
NADPH
recombinant mutant Q35S, pH 7.4, 37°C
0.004
NADPH
-
recombinant enzyme
0.004
NADPH
-
pH 7.4, 37°C
0.004
NADPH
pH 7.4, 37°C, wild-type enzyme
0.0042
NADPH
-
pH 7.3, 37°C
0.0043
NADPH
-
recombinant from E. coli
0.0043
NADPH
-
mutant L20M from plasmid
0.0043
NADPH
mutant Y35G/F37L, pH 5.0, 25°C
0.0044
NADPH
pH 7.4, 37°C, mutant Q35S
0.00443
NADPH
-
wild type enzyme, in 50 mM TES, pH 7.0, at 37°C
0.0045
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
0.0045
NADPH
-
mutant W48Y, 25°C, pH 7.0
0.00456
NADPH
-
triple mutant L16M/L20M/L42M from plasmid
0.0046
NADPH
25°C, pH 7.0, pressure 200 MPa
0.0046
NADPH
pH 7.4, 37°C, mutant Q35S/N64S
0.0048
NADPH
mutant Y35L/F37T, pH 5.0, 25°C
0.0048
NADPH
-
pH 7, 20°C, wild-type enzyme
0.005
NADPH
-
recombinant wild-type enzyme
0.0052
NADPH
pH 7.4, 37°C, mutant N64F
0.00523
NADPH
-
mutant enzyme T86N, in 50 mM TES, pH 7.0, at 37°C
0.0053
NADPH
recombinant mutant Q35S/N64S, pH 7.4, 37°C
0.0053
NADPH
-
pH 8.0, 25°C, presence of 200 mM NaCl
0.0055
NADPH
mutant Q134K, pH 8.5, 25°C
0.0055
NADPH
pH 7.4, 37°C, mutant Q35K/N64F
0.0056
NADPH
recombinant mutant Q35S/N64F, pH 7.4, 37°C
0.0057
NADPH
recombinant mutant Q35K/N64F, pH 7.4, 37°C
0.0057
NADPH
-
pH 8.0, 25°C, presence of 1000 mM NaCl
0.0058
NADPH
wild-type, pH 5.0, 25°C
0.0059
NADPH
-
with 7,8-dihydrofolate or 6-hydroxymetylpterin
0.0059
NADPH
Halalkalibacterium halodurans
-
His-tagged recombinant enzyme, pH 7.5, 37°C
0.0059
NADPH
Halalkalibacterium halodurans
-
His-tagged enzyme, pH 7.5, 37°C
0.00595
NADPH
-
mutant N51I/C59R/N108T/I164L, pH 7.0
0.0063
NADPH
pH 7.4, 37°C, mutant N64S
0.0063
NADPH
recombinant mutant N64S, pH 7.4, 37°C
0.0064
NADPH
-
pH 8.0, 25°C
0.00643
NADPH
-
mutant N51I/C59R/S108N/I164L, pH 7.0
0.0065
NADPH
-
His-tagged human-derived enzyme, pH 7.65
0.0065
NADPH
-
mutant N51I/C59R/S108N/I164L/D187A, pH 7.0
0.0066
NADPH
-
pH 6.0, 25°C
0.0066
NADPH
recombinant mutant K37S, pH 7.4, 37°C
0.0066
NADPH
recombinant mutant Q35K/N64S, pH 7.4, 37°C
0.0068
NADPH
mutant K27E, pH 5.0, 25°C
0.0069
NADPH
wild-type, pH 8.5, 25°C
0.007
NADPH
-
recombinant mutant S108N
0.007
NADPH
recombinant mutant K37S/F69S, pH 7.4, 37°C
0.0071
NADPH
mutant L30Q, pH 8.5, 25°C
0.0072
NADPH
-
mutant N51I/C59R/S108N/I164L/K96N, pH 7.0
0.0072
NADPH
recombinant mutant K37Q, pH 7.4, 37°C
0.0074
NADPH
pH 7.5, 25°C
0.0074
NADPH
pH 7.5, temperature not specified in the publication, cosubstrate: 10-formyl-dihydrofolate
0.0075
NADPH
recombinant mutant N64F, pH 7.4, 37°C
0.00792
NADPH
-
mutant enzyme T86S, in 50 mM TES, pH 7.0, at 37°C
0.008
NADPH
pH 7.5, temperature not specified in the publication, cosubstrate: 7,8-dihydrofolate
0.0081
NADPH
-
pH 6.0, 25°C, presence of 500 mM NaCl
0.0082
NADPH
Halalkalibacterium halodurans
-
wild-type, pH 7.5, 37°C
0.009
NADPH
-
recombinant mutants S108G, S108A and S108C
0.0091
NADPH
-
pH 7.3, 37°C
0.0092
NADPH
-
pH 7.4, 37°C, mutant K37Q
0.0096
NADPH
pH 7.0, 30°C
0.0097
NADPH
mutant Y35L/F37R, pH 5.0, 25°C
0.00975
NADPH
25°C, recombinant detagged enzyme
0.0104
NADPH
-
pH 7.0, 60°C
0.0105
NADPH
mutant Y35Q/F37R, pH 5.0, 25°C
0.0106
NADPH
recombinant mutant K37Q/F69S, pH 7.4, 37°C
0.011
NADPH
-
with L-threo-neopterin
0.011
NADPH
-
mutant carrying the K32M mutation in gene copies 1 and 3 with the Q67H mutation in all gene copies, pH 7.0, 30°C
0.0113
NADPH
-
mutant N51I/C59R/S108N/I164L/I150V/N182I/N201D, pH 7.0
0.0118
NADPH
recombinant mutant S37Q, pH 7.4, 37°C
0.012
NADPH
-
25°C, pH 7.4
0.0123
NADPH
pH 7.0, 20°C
0.0123
NADPH
-
recombinant rat-derived enzyme, pH 7.65
0.0125
NADPH
recombinant mutant Q35K, pH 7.4, 37°C
0.013
NADPH
pH 7.4, 37°C, mutant Q35K
0.014
NADPH
-
recombinant mutant S108Q
0.0145
NADPH
-
pH 7.4, 37°C, wild-type enzyme
0.0149
NADPH
recombinant mutant S37K/S69F, pH 7.4, 37°C
0.0157
NADPH
wild-type, pH 7.0, 25°C
0.0162
NADPH
recombinant mutant S69F, pH 7.4, 37°C
0.0163
NADPH
recombinant mutant F69N, pH 7.4, 37°C
0.0163
NADPH
recombinant mutant K37Q/F69N, pH 7.4, 37°C
0.0167
NADPH
-
native rat-derived enzyme, pH 7.65
0.0167
NADPH
recombinant mutant K37S/F69N, pH 7.4, 37°C
0.017
NADPH
-
recombinant mutant S108T
0.017
NADPH
25°C, recombinant GST-tagged enzyme
0.0173
NADPH
recombinant mutant S37Q/S69N, pH 7.4, 37°C
0.018
NADPH
Tequatrovirus T4
-
-
0.0189
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
0.0192
NADPH
recombinant mutant F69S, pH 7.4, 37°C
0.0195
NADPH
-
pH 7, 20°C, monomeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0198
NADPH
pH 7.0, 25°C, under atmospheric pressure
0.0199
NADPH
-
pH 7, 20°C, dimeric variant (Xet-3) of dihydrofolate reductase from Escherichia coli prepared by introducing residues located at the Thermotoga maritima dihydrofolate reductase dimer interface
0.0229
NADPH
-
pH 7.4, 37°C, wild-type enzyme
0.023
NADPH
recombinant wild-type enzyme, pH 7.4, 37°C
0.0232
NADPH
-
wild-type, pH 8.0, 25°C
0.0252
NADPH
recombinant mutant S69N, pH 7.4, 37°C
0.0267
NADPH
-
mutant I51N/C59R/N108S/I164L, pH 7.0
0.0275
NADPH
recombinant mutant S37Q/S69F, pH 7.4, 37°C
0.029
NADPH
-
mutant isoform that carries the K32M mutation in gene copies 1 and 3 and the Q67H mutation in gene copies 2 and 4, pH 7.0, 30°C
0.0373
NADPH
-
mutant N51I/C59R/S108N/I164L , pH 8.0, 25°C
0.0379
NADPH
-
mutant N51I/C59R/S108N, pH 8.0, 25°C
0.052
NADPH
-
mutant N51I/C59R/N108S/I164L, pH 7.0
0.0584
NADPH
pH 7.0, 25°C, under atmospheric pressure
0.06
NADPH
-
wild-type enzyme
0.067
NADPH
-
at 25 °C and 100 mM HEPES, pH 7.5
0.07
NADPH
-
mutant enzyme
0.0864
NADPH
recombinant mutant S37K/S69N, pH 7.4, 37°C
0.101
NADPH
pH 7.0, 25°C, under atmospheric pressure
additional information
additional information
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-
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
additional information
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additional information
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additional information
additional information
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additional information
additional information
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additional information
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additional information
additional information
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-
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additional information
additional information
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-
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additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
Tequatrovirus T4
-
-
-
additional information
additional information
Tequatrovirus T4
-
-
-
additional information
additional information
Tequatrovirus T4
-
-
-
additional information
additional information
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-
-
additional information
additional information
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-
-
additional information
additional information
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-
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additional information
additional information
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additional information
additional information
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-
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additional information
additional information
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-
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
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overview
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additional information
additional information
protozoa
-
overview
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additional information
additional information
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overview Km-values of wild-type and mutant enzymes decreasing with increasing concentration of KCl
-
additional information
additional information
-
buffer and salt conditions significantly alter the Km-values for the substrates
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
protozoa
-
Km of enzymes with decreased binding of folate and antagonists and of drug-sensitive enzymes
-
additional information
additional information
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selenomethionine-containing mutants
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
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Michaelis-Menten kinetics
-
additional information
additional information
Michaelis-Menten kinetics
-
additional information
additional information
-
Michaelis-Menten kinetics
-
additional information
additional information
analysis of steady-state kinetics at pH 9.5
-
additional information
additional information
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analysis of steady-state kinetics at pH 9.5
-
additional information
additional information
steady-state kinetics of wild-type and mutant enzymes
-
additional information
additional information
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steady-state kinetics of wild-type and mutant enzymes
-
additional information
additional information
transient kinetics investigation of enzyme
-
additional information
additional information
enzyme activity decreases with increasing pressure. The Km values for dihydrofolate and NADPH are slightly higher at 200 MPa than at atmospheric pressure. The hydride transfer step is insensitive to pressure, while the dissociation constants of substrates and cofactors increase with pressure
-
additional information
additional information
-
enzyme activity decreases with increasing pressure. The Km values for dihydrofolate and NADPH are slightly higher at 200 MPa than at atmospheric pressure. The hydride transfer step is insensitive to pressure, while the dissociation constants of substrates and cofactors increase with pressure
-
additional information
additional information
molecular dynamics and spectroscopic analysis of the enzyme in transition state based on residues Tyr100 and Tyr111, overview
-
additional information
additional information
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Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
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Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
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Michaelis-Menten steady-state kinetics
-
additional information
additional information
Michaelis-Menten steady-state kinetics
-
additional information
additional information
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Michaelis-Menten steady-state kinetics
-
additional information
additional information
kinetic behavior of the wild-type enzyme ecDHFR and enzyme mutants D27S, Y100F, and D27S/Y100F across an extended pH range, including pH 9.0. Competitive hydrogen to deuterium and hydrogen to tritium kinetic isotope effects (KIEs) on the second-order rate constant (kcat/Km) are measured for wild-type and mutant ecDHFR variants to assess the intrinsic KIE on the catalyzed hydride transfer. Presteady-state, Michaelis-Menten, and single-turnover kinetics
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additional information
additional information
-
kinetic behavior of the wild-type enzyme ecDHFR and enzyme mutants D27S, Y100F, and D27S/Y100F across an extended pH range, including pH 9.0. Competitive hydrogen to deuterium and hydrogen to tritium kinetic isotope effects (KIEs) on the second-order rate constant (kcat/Km) are measured for wild-type and mutant ecDHFR variants to assess the intrinsic KIE on the catalyzed hydride transfer. Presteady-state, Michaelis-Menten, and single-turnover kinetics
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additional information
additional information
single turnover, stopped-flow, and steady-state kinetics
-
additional information
additional information
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single turnover, stopped-flow, and steady-state kinetics
-
additional information
additional information
kinetics by non-tight binding Michaelis-Menten model
-