1.5.1.11: D-octopine dehydrogenase
This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.
Reaction
Synonyms
D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008
ECTree
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Reaction
Reaction on EC 1.5.1.11 - D-octopine dehydrogenase
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kinetic mechanism
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
NADH binds to the enzyme first followed by L-Arg and pyruvate, which bind randomly
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
bi-ter sequential mechanism where NAD+ binds first to the enzyme followed by D-octopine, and the products are released in the order L-Arg, pyruvate and NADH
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
partially random mechanism in which NADH is the obligatory first substrate, and Arg and pyruvate bind randomly to the enzyme-NADH complex. Ordered sequential addition of NAD+ and octopine in octopine oxidation
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
in the direction of octopine oxidation NAD binds to the enzyme before octopine in a rapid equilibrium fashion. The products L-Arg and pyruvate are released in a random fashion
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
partially ordered mechanism. NADPH binds first followed by the random order, rapid-equilibrium-binding of Arg and pyruvate. Orn probably binds before pyruvate
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
the enzyme removes the pro-S hydrogen atom of the dihydronicotinamide ring with transfer of label to the solvent and to the product octopine (N-2-(1-carboxyethyl)-L-arginine )
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
the enzyme removes the pro-S hydrogen atom of the dihydronicotinamide ring with transfer of label to the solvent and to the product octopine (N-2-(1-carboxyethyl)-L-arginine )
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N2-(D-1-carboxyethyl)-L-arginine + NAD+ + H2O = L-arginine + pyruvate + NADH + H+
substrates bind in an ordered sequential manner. First NADH binds to OcDH followed by L-arginine. The binding of the guanidinium headgroup of L-arginine induces a conformational change, resulting in the formation of the pyruvate binding site. The reduction of pyruvate can only occur in the presence of L-arginine, which than forms octopine and prevents lactate formation