1.5.1.11: D-octopine dehydrogenase
This is an abbreviated version!
For detailed information about D-octopine dehydrogenase, go to the full flat file.
Reaction
Synonyms
D-Octopine synthase, Dehydrogenase, octopine, flavin-containing opine dehydrogenase, N2-(D-1-carboxyethyl)-L-arginine:NAD+ oxidoreductase, N2-(D-1-carboxyethyl)-L-arginine:NAD+-oxidoreductase, OcDH, OCS, Octopine dehydrogenase, Octopine synthase, Octopine:NAD oxidoreductase, ODH, pTi_008
ECTree
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KM Value
KM Value on EC 1.5.1.11 - D-octopine dehydrogenase
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5.9
2-oxobutyrate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
49.8
2-oxovalerate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
1.4 - 2.8
Arg
-
dependent on concentration of the cosubstrates NADH and pyruvate
0.5
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
0.5
L-Arg
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
1.6
L-Arg
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
1.7
L-Arg
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
3.5
L-Arg
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
5.8
L-Arg
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
9.8
L-Arg
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
81
L-Arg
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
159
L-Arg
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
1.4
-
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
1.8
L-lysine
-
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
1.8
L-lysine
-
recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.0198
NADH
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
0.0198
NADH
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.0214
NADH
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.024
NADH
-
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.026
NADH
-
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.028
NADH
-
recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.0389
NADH
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.04
NADH
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.0649
NADH
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.0904
NADH
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
0.104
NADH
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
0.175
NADH
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
0.25
pyruvate
-
crude extract enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.35
pyruvate
-
GST-tagged enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.35
pyruvate
-
recombinant enzyme, in 100 mM citrate phosphate buffer, pH 6.2, temperature not specified in the publication
0.5 - 1.5
pyruvate
-
depending on concentration of the cosubstrates NADH and Arg
0.77
pyruvate
wild type enzyme, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
0.8
pyruvate
wild type recombinant enzyme, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
2.5
pyruvate
mutant enzyme C148A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
2.6
pyruvate
mutant enzyme C148S, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
27
pyruvate
mutant enzyme Q118A, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
33
pyruvate
mutant enzyme D329A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
59
pyruvate
mutant enzyme H212A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
92
pyruvate
mutant enzyme R324A, in 50 mM triethanolamine hydrochloride buffer pH 7.0, at 25°C
162
pyruvate
mutant enzyme Q118D, in 50 mM triethanolamine buffer, pH 7.0, at 25°C
additional information
additional information
-
dependence of the Km-values for Arg and pyruvate on the concentration of the other substrates
-
additional information
additional information
-
temperature-dependence of Km-values
-