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1.4.4.2: glycine dehydrogenase (aminomethyl-transferring)

This is an abbreviated version!
For detailed information about glycine dehydrogenase (aminomethyl-transferring), go to the full flat file.

Word Map on EC 1.4.4.2

Reaction

glycine
+
[glycine-cleavage complex H protein]-N6-lipoyl-L-lysine
=
[glycine-cleavage complex H protein]-S-aminomethyl-N6-dihydrolipoyl-L-lysine
 +
CO2

Synonyms

decarboxylase, glycine, GDC, GDCP, GLDC, GLDH, GLDP, Gly decarboxylase complex, Gly decarboxylase H1, glycine cleavage enzyme complex, glycine cleavage H protein, glycine cleavage system H protein 1, Glycine cleavage system P-protein, glycine decarboxylase, glycine decarboxylase (P-protein), glycine decarboxylase complex, glycine decarboxylase complex H, glycine decarboxylase P-protein, glycine dehydrogenase, glycine dehydrogenase (decarboxylating), glycine-cleavage complex, H protein, H-protein, H1 protein, H2 protein, L protein, More, P protein, P-protein, P-protein (glycine decarboxylase), P-subunit, Protein P1, T protein

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.4 With a disulfide as acceptor
                1.4.4.2 glycine dehydrogenase (aminomethyl-transferring)

Cloned

Cloned on EC 1.4.4.2 - glycine dehydrogenase (aminomethyl-transferring)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Arabidopsis thaliana and Moricandia arvensis using Agrobacterium tumefaciens strain GV3101
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli strains BL21 Gold and LMG194
-
expressed in Flaveria bidentis (C4) and Arabidopsis thaliana (C3)
-
expressed in Pichia pastoris strain GS115
expression of P-protein in yeast strains DS2-73U and DS2-75U
-
expression of the His-tagged enzyme in Escherichia coli
gene GLDC, DNA and amino acid sequence determination and analysis, genotyping
into pGEM-T vector for DNA manipulations and sequence analysis using the Escherichia coli strain TG1
-
into the pET-29B vector for expression in Escherichia coli BL21DE3 cells, into master-neo(HA)2 plasmid for transfection of Trichomonas vaginalis
-
missense mutations expressed in COS7 cells
-
pET-H1, pET-H2, and pET-L plasmids expressed in Escherichia coli BL21(DE3) cells
-
recombinant expression of codon optimized C-terminally His6-tagged enzyme GLDC in Escherichia coli strain BL21(DE3), GLDC is expressed either separately (without H-protein) or simultaneously with H-protein, coexpression of His6-tagged enzyme with codon-optimized human H-protein in Escherichia coli strain BL21(DE3) from pJexpress-416 vector
two identified mutations, A389V and R739V, are introduced into the pEUK-(N) expression vector pEUK-(C1) carrying human glycine decarboxylase complementary DNA for expression in COS7 cells
-