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1.4.3.5: pyridoxal 5'-phosphate synthase

This is an abbreviated version!
For detailed information about pyridoxal 5'-phosphate synthase, go to the full flat file.

Word Map on EC 1.4.3.5

Reaction

pyridoxamine 5'-phosphate
+
H2O
 +
O2
=
pyridoxal 5'-phosphate
 +
NH3
 +
H2O2

Synonyms

ePNPOx, FprA protein, oxidase, pyridoxamine phosphate, Pdx1, PDX3, PdxH, PMP oxidase, PNP oxidase, PNP/PMP oxidase, PNPO, PNPOx, PPOX, pyridox(am)ine 5'-phosphate oxidase, pyridoxal 5'-phosphate synthase, pyridoxal 5'-phosphate synthetic enzyme, pyridoxamine (pyridoxine) 5'-phosphate oxidase, pyridoxamine (pyridoxine) 5’-phosphate:O2 oxidoreductase (deaminating), pyridoxamine 5'-phosphate oxidase, pyridoxamine phosphate oxidase, pyridoxamine-5-phosphate oxidase, pyridoxamine-phosphate oxidase, pyridoxaminephosphate oxidase (EC 1.4.3.5: deaminating), pyridoxinamine 5'-phosphate oxidase, pyridoxine (pyridoxamine) 5 '-phosphate oxidase, pyridoxine (pyridoxamine) 5'-phosphate oxidase, pyridoxine (pyridoxamine) phosphate oxidase, pyridoxine 5'-phosphate oxidase, pyridoxine 5'-phosphate oxidase`, pyridoxine 5-phosphate oxidase, pyridoxine phosphate oxidase, pyridoxine-5'-phosphate oxidase, pyridoxine/pyridoxamine 5'-phosphate oxidase, pyridoxine/pyridoxamine phosphate oxidase, sgll, Sgll/PNPO, Ylr456w, Ypr172w

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.5 pyridoxal 5'-phosphate synthase

Purification

Purification on EC 1.4.3.5 - pyridoxal 5'-phosphate synthase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
acid treatment, ammonium sulfate, DEAE-Sephadex, Sephadex G-100, phosphopyridoxal-Sepharose
-
affinity chromatography
His Trap column chromatography and Superdex 200 gel filtration
-
HisTrap nickel-affinity column chromatography and Superdex 200 gel filtration
native enzyme 3175fold from brain by pyridoxyl and phosphopyridoxyl affinity chromatography, gel filtration, and anion exchange chromatography, to homogeneity
-
native enzyme 556fold from liver by acid and ethanol precipitations, anion exchange chromatography, gel filtration, and calcium phosphate gel chromatography
-
native enzyme from liver
-
native enzyme to homogeneity by calcium phosphate resin and anion exchange chromatography, and gel filtration, separation of isozymes PMP oxidase and PNP oxidase on calcium phosphate gel
-
native enzyme with tightly bound FMN
Ni-NTA column chromatography
-
nickel affinity column chromatography
-
partial
partially from 7-days-old seedlings, preparation of acetone powder
-
recombinant enzyme from Escherichia coli strain BL21 (DE3) to homogeneity
recombinant enzyme, DEAE-cellulose, hydroxyapatite, CM-cellulose, Bio-Gel
-
recombinant GST-tagged enzyme from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tag by rTEV protease, followed by dialysis and ultrafiltration
recombinant GST-tagged wild-type and of GST-tagged selenomethionine-labeled enzymes from Escherichia coli strain BL21(DE3) by glutathione affinity chromatography, removal of the GST-tags by rTEV protease, followed by dialysis and ultrafiltration
-
recombinantly expressed mutant enzyme R118Q
two isozymes from seedlings, partially by anion exchange and adsorption chromatography
-