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0.0059
5'-homopyridoxine-phosphate
-
-
0.0059 - 0.031
5'-methylpyridoxine-phosphate
0.025
5'-phospho-pyridoxal-O-carboxymethyloxime
-
-
0.021
5'-phospho-pyridoxaloxime
-
-
0.01
5'-phospho-pyridoxyl-3-aminobenzoate
-
-
0.035
5'-phospho-pyridoxyl-3-L-alanine
-
-
-
0.01
5'-phospho-pyridoxyl-4-aminobenzoate
-
-
0.03
5'-phospho-pyridoxyl-4-aminobutyrate
0.03
5'-phospho-pyridoxyl-5-aminovalerate
-
-
0.01
5'-phospho-pyridoxyl-aniline
-
-
0.2
alpha-N-(5'-phospho-4'-pyridoxyl)-L-lysine
-
-
-
0.53
alpha-N-(5'-phospho-4'-pyridoxyl)-ornithine
-
-
0.033
N-(5'-phospho-4'-pyridoxyl)-benzylamine
-
-
0.11
N-(5'-phospho-4'-pyridoxyl)-beta-L-alanine
-
-
-
0.77
N-(5'-phospho-4'-pyridoxyl)-D-alanine
-
-
0.29
N-(5'-phospho-4'-pyridoxyl)-D-alpha-aminobutyrate
-
-
0.125
N-(5'-phospho-4'-pyridoxyl)-D-leucine
-
-
1.6
N-(5'-phospho-4'-pyridoxyl)-D-tyrosine
-
-
0.068
N-(5'-phospho-4'-pyridoxyl)-glycine
-
-
0.22
N-(5'-phospho-4'-pyridoxyl)-L-alanine
-
-
0.091
N-(5'-phospho-4'-pyridoxyl)-L-alpha-aminobutyrate
-
-
0.075
N-(5'-phospho-4'-pyridoxyl)-L-leucine
-
-
0.095
N-(5'-phospho-4'-pyridoxyl)-L-phenylalanine
-
-
0.13
N-(5'-phospho-4'-pyridoxyl)-L-serine
-
-
0.12
N-(5'-phospho-4'-pyridoxyl)-L-tryptophan
-
-
0.031
N-(5'-phospho-4'-pyridoxyl)-L-tyrosine
-
-
0.008
N-(5'-phospho-4'-pyridoxyl)-N'-(1-naphthyl)ethylenediamine
-
-
0.00065
N-Phosphopyridoxyl tryptamine
-
-
0.4
N10-(5'-phospho-4'-pyridoxyl)-1,10-diaminodecane
-
-
0.065
N2-acetyl-N6-(5'-phospho-pyridoxyl)-L-lysine
-
-
0.368
pyridoxal 5'-phosphate
-
recombinant enzyme after cleavage from fusion protein
0.021
pyridoxal 5'-phosphate oxime
-
-
0.025
pyridoxal 5'-phosphate-carboxymethyloxime ester
-
-
-
0.0013 - 1
pyridoxamine 5'-phosphate
0.1
pyridoxamine-5'-phosphate
-
-
0.0003 - 2.4
pyridoxine 5'-phosphate
additional information
additional information
-
0.0059
5'-methylpyridoxine-phosphate
-
-
0.031
5'-methylpyridoxine-phosphate
-
-
0.03
5'-phospho-pyridoxyl-4-aminobutyrate
-
-
0.03
5'-phospho-pyridoxyl-4-aminobutyrate
-
-
0.000016
FMN
-
-
0.085
O2
-
cosubstrate pyridoxamine 5'-phosphate
0.182
O2
-
cosubstrate pyridoxine 5'-phosphate
0.0013
pyridoxamine 5'-phosphate
-
pH 8.0
0.0013
pyridoxamine 5'-phosphate
-
measured in hemolysate
0.0021
pyridoxamine 5'-phosphate
-
pH 8.0, 25°C, wild-type enzyme
0.0036
pyridoxamine 5'-phosphate
-
-
0.0062
pyridoxamine 5'-phosphate
-
pH 8.0, 25°C, mutant enzyme DELTA1-56
0.0067
pyridoxamine 5'-phosphate
-
isozyme E-II, pH 8.0, 37°C
0.008
pyridoxamine 5'-phosphate
-
enzyme activity in liver homogenate
0.01
pyridoxamine 5'-phosphate
-
-
0.01
pyridoxamine 5'-phosphate
-
-
0.01
pyridoxamine 5'-phosphate
-
immobilized enzyme
0.011
pyridoxamine 5'-phosphate
-
enzyme activity in liver homogenate
0.0112
pyridoxamine 5'-phosphate
-
-
0.0112
pyridoxamine 5'-phosphate
-
pH 8.0, 37°C
0.013
pyridoxamine 5'-phosphate
-
-
0.015
pyridoxamine 5'-phosphate
-
enzyme activity in cytosol
0.018
pyridoxamine 5'-phosphate
-
soluble enzyme
0.018
pyridoxamine 5'-phosphate
-
pH 8.0, 37°C
0.0187
pyridoxamine 5'-phosphate
-
in the presence of 1 M urea
0.0187
pyridoxamine 5'-phosphate
-
pH 8.0, 37°C, in presence of 1 M urea
0.024
pyridoxamine 5'-phosphate
-
pure enzyme in 200 mM potassium phosphate pH 7.0
0.024
pyridoxamine 5'-phosphate
-
pH 7.0, 37°C
0.025
pyridoxamine 5'-phosphate
-
enzyme species FI which is assumed to be an artefact of the purification procedure
0.039
pyridoxamine 5'-phosphate
-
enzyme activity in liver homogenate in the presence of 0.005 mM pyridoxal 5'-phosphate
0.0417
pyridoxamine 5'-phosphate
-
in the presence of 2 M urea
0.0417
pyridoxamine 5'-phosphate
-
pH 8.0, 37°C, in presence of 2 M urea
0.105
pyridoxamine 5'-phosphate
-
-
0.105
pyridoxamine 5'-phosphate
-
recombinant enzyme
0.14
pyridoxamine 5'-phosphate
-
-
0.4
pyridoxamine 5'-phosphate
-
recombinant enzyme after cleavage from fusion protein
1
pyridoxamine 5'-phosphate
-
pH 7.6, 37°C
0.0003
pyridoxine 5'-phosphate
-
0.0003
pyridoxine 5'-phosphate
H199N mutant enzyme
0.00092
pyridoxine 5'-phosphate
-
-
0.001
pyridoxine 5'-phosphate
D49A and Y17F mutant enzyme
0.0012
pyridoxine 5'-phosphate
-
immobilized enzyme
0.0016
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme
0.0017
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L
0.002
pyridoxine 5'-phosphate
-
-
0.002
pyridoxine 5'-phosphate
-
recombinant enzyme
0.002
pyridoxine 5'-phosphate
R14E mutant enzyme
0.002
pyridoxine 5'-phosphate
-
pH 8.5, 37°C
0.0021
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L/R24L
0.0024
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme K145A/F177A
0.0026
pyridoxine 5'-phosphate
R14M mutant enzyme
0.0026
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R215L
0.0026
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme, TRIS buffer
0.0027
pyridoxine 5'-phosphate
-
soluble enzyme
0.0028
pyridoxine 5'-phosphate
pH 7.6, 37°C, wild-type enzyme, HEPES buffer
0.0031
pyridoxine 5'-phosphate
-
-
0.0031
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme N84W/K145A/F177A
0.0031
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R116Q
0.0032
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme N84A/K145A/F177A
0.0032
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L/R215L
0.0034
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R23L/R24L/R215L
0.004
pyridoxine 5'-phosphate
-
enzyme activity in liver homogenate
0.0051
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R116Q/R225H
0.0057
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme G118R
0.0061
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R141C
0.00618
pyridoxine 5'-phosphate
wild type enzyme, at pH 8.0 and 37°C
0.0062
pyridoxine 5'-phosphate
-
pH 8.0, 25°C, wild-type enzyme
0.008
pyridoxine 5'-phosphate
-
isozyme E-II, pH 8.0, 37°C
0.0082
pyridoxine 5'-phosphate
-
-
0.009
pyridoxine 5'-phosphate
-
enzyme species FI which is assumed to be an artefact of the purification procedure
0.013
pyridoxine 5'-phosphate
-
-
0.016
pyridoxine 5'-phosphate
-
pure enzyme in 200 mM potassium phosphate pH 7.0
0.016
pyridoxine 5'-phosphate
-
pH 7.0, 37°C
0.016
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R225H
0.017
pyridoxine 5'-phosphate
-
enzyme activity in cytosol
0.017
pyridoxine 5'-phosphate
-
enzyme activity in liver homogenate
0.02
pyridoxine 5'-phosphate
-
-
0.02
pyridoxine 5'-phosphate
-
isozyme E-I, pH 8.0, 37°C
0.0208
pyridoxine 5'-phosphate
-
pH 8.0, 25°C, mutant enzyme DELTA1-56
0.022
pyridoxine 5'-phosphate
-
enzyme activity in liver homogenate in the presence of 0.005 mM pyridoxal 5'-phosphate
0.0238
pyridoxine 5'-phosphate
-
-
0.0238
pyridoxine 5'-phosphate
-
pH 8.0, 37°C
0.025
pyridoxine 5'-phosphate
-
pH 8.0, 37°C
0.03
pyridoxine 5'-phosphate
-
-
0.037
pyridoxine 5'-phosphate
-
wild type enzyme, at pH 8.0 and 37°C
0.0667
pyridoxine 5'-phosphate
-
in the presence of 2 M urea
0.0667
pyridoxine 5'-phosphate
-
pH 8.0, 37°C, in presence of 2 M urea
0.07
pyridoxine 5'-phosphate
H199A mutant enzyme
0.09
pyridoxine 5'-phosphate
R197M mutant enzyme
0.113
pyridoxine 5'-phosphate
-
mutant enzyme K72P, at pH 8.0 and 37°C
0.219
pyridoxine 5'-phosphate
-
mutant enzyme K72R, at pH 8.0 and 37°C
0.36
pyridoxine 5'-phosphate
-
in 25 mM potassium phosphate, pH 7.8 and 25°C
0.436
pyridoxine 5'-phosphate
mutant enzyme R95C, at pH 8.0 and 37°C
0.436
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R95C
0.461
pyridoxine 5'-phosphate
pH 7.6, 37°C, mutant enzyme R229W
0.625
pyridoxine 5'-phosphate
-
mutant enzyme K72A, at pH 8.0 and 37°C
0.946
pyridoxine 5'-phosphate
-
mutant enzyme K72Q, at pH 8.0 and 37°C
1.8
pyridoxine 5'-phosphate
-
pH 7.6, 37°C
2.4
pyridoxine 5'-phosphate
R197M mutant enzyme
additional information
additional information
-
-
-
additional information
additional information
-
steady-state kinetics
-
additional information
additional information
-
KM-values of mutant enzymes H199A, H199N, R197E, R197M, Y17F, R14E, R14M
-
1.4.3.5 pyridoxal 5'-phosphate synthase
results (
results (
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