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1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

Specific Activity

Specific Activity on EC 1.4.3.3 - D-amino-acid oxidase

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SPECIFIC ACTIVITY [µmol/min/mg]
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
0.0004
-
specific activity in liver, at 37°C
0.00045
-
specific activity in liver, at 37°C
0.0007
-
specific activity in brain, at 37°C
0.0018
-
cell-free extract from tadpoles, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C
0.0082
-
activity in F344 Fischer rat kidney
0.0106
-
activity in Sprague-Dawley rat kidney
0.0135
-
cell-free extract from adults with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C
0.014
-
specific activity in kidney, at 37°C
0.087
-
specific activity in kidney, at 37°C
0.15
-
-
0.165
-
enzyme from adults after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C
0.641
-
enzyme from tadpoles after 3563fold purification, with 20 mM D-proline in 20 mM Tris-HCl (pH 8.5) at 37°C
100
mutants R120E and R120L, substrate D-alanine, pH 8.5, 25°C
104
purified wild type enzyme, at 25°C
112
substrate D-isoleucine, 55°C, pH 8.0
18.61
-
wild-type
180
recombinant enzyme after 6fold purification, for D-methionine, at 30°C
2.2
-
recombinant Strep-tagged wild-type enzyme, substrates are D-methionine and 6-dichloroindophenolate, pH 7.5, 37°C
2.6
-
recombinant Strep-tagged mutant R101A, substrates are D-methionine and 6-dichloroindophenolate, pH 7.5, 37°C
21.5
mutant Y228L/R283G, substrate (1R)-1-phenylethan-1-amine, pH not specified in the publication, temperature not specified in the publication
22.3
purified recombinant mutant F54S, substrate cephalosporin C, pH 7.5, 22°C
26.4
purified recombinant mutant F54A, substrate cephalosporin C, pH 7.5, 22°C
27
immobiliuzed enzyme
30
recombinant enzyme from crude extract, for D-methionine, at 30°C
30.6
purified recombinant mutant F54Y, substrate cephalosporin C, pH 7.5, 22°C
4.6
-
recombinant polyethylene glycol-conjugated D-amino acid oxidase, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2)
5.3
-
native enzyme, at 25°C in 0.1 M Tris-HCl buffer (pH 8.2)
61
-
immobilized enzyme, at 25°C
70
substrate D-isoleucine, 37°C, pH 8.0
72
-
native enzyme, with D-alanine as substrate, at 37°C
77
-
double fusion enzyme, with D-alanine as substrate, at 37°C
8
-
purified recombinant enzyme
8.5
purified recombinant wild-type enzyme, substrate cephalosporin C, pH 7.5, 22°C
8.8
-
-
80
wild-type, substrate D-alanine, pH 8.5, 25°C
95
-
native enzyme, with D-alanine as substrate, at 37°C
96
-
double fusion enzyme, with D-alanine as substrate, at 37°C
additional information