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1.4.3.3: D-amino-acid oxidase

This is an abbreviated version!
For detailed information about D-amino-acid oxidase, go to the full flat file.

Word Map on EC 1.4.3.3

Reaction

a D-amino acid
+
H2O
 +
O2
=
a 2-oxo carboxylate
 +
NH3
 +
H2O2

Synonyms

chDAO, D-AAO, D-amino acid oxidase, D-amino-acid-oxidase, D-aminoacid oxidase, DAAO, DAMOX, DAO, DAO1, DaoE, hDAAO, ophio-amino-acid oxidase, oxidase, D-amino acid, PEG-DAO, pkDAAO, RgDAAO, TvDAAO, TvDAO,  LH99

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.3 With oxygen as acceptor
                1.4.3.3 D-amino-acid oxidase

Cloned

Cloned on EC 1.4.3.3 - D-amino-acid oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
cDNA library screening
DNA and amino acid sequence determination and analysis, sequence comparison, expression of the enzyme fused to the maltose-binding protein in Escherichia coli strain TB1
expressed in Arabidopsis thaliana and in Escherichia coli DH5alpha cells
-
expressed in Escherichia coli
expressed in Escherichia coli BL21 (DE3) cells
-
expressed in Escherichia coli BL21(DE3) cells
expressed in Escherichia coli BL21(DE3) CodonPlus/pLysS cells
-
expressed in Escherichia coli BL21(DE3) or BL21 Star(DE3) cells
expressed in Escherichia coli BL21(DE3)pLysS cells
expressed in Escherichia coli BL21(DE3)Star cells
-
expressed in Escherichia coli strain BL21
expressed in Escherichia coli strain BL21 (DE3)
-
expressed in Escherichia coli strain BL21(DE3) and HEK293-H cells
expressed in Escherichia coli strain BL21(DE3)pLysS and in Nicotiana tabacum plastids
-
expressed in Escherichia coli strains BL21(DE3), TOP10FÂ’, JM105, JM109, and BL21
-
expressed in Escherichia coli Top10F', JM109, JM105, and BL21 cells
-
expressed in Escherichia coli Tunner(DE3) cells
expressed in glioblastoma cells
expressed in HeLa cells
-
expression in CHO cells
expression in COS-1, NRK-52E and CCL-PK1 cells
-
expression in Escherichia coli
expression in Escherichia coli and in U87 cell
expression in Escherichia coli strain BL21(DE3), optimization of culture condition for the recombinant production of D-amino acid oxidase in Escherichia coli, overview
-
expression in Escherichia coli. TvDAO can be produced in Escherichia coli as a fully functional recombinant protein. They also show that microheterogeneity due to modification of Cys108 is avoided completely during production and purification
-
expression in Kluyveromyces lactis
-
expression in Pichia pastoris strain CBS 7435, the enzyme production is enhanced to 350 kUnits/l of fed-batch bioreactor culture with a productivity of 3.1 kUnits/l h, subcloning in Escherichia coli. Expression optimzation and quantitative real-time PCR analysis expression analysis,overview
expression in Saccharomyces cerevisiae
-
expression in SH-SY5Y, 1321-N1 and HEK-293 cells
-
expression of DAAO in Spodoptera frugiperda Sf9 cells
expression of EGFP-tagged DAAO in U-87 glioblastoma cells, transient coexpression of pLG72
expression of His-tagged DAO (HDAO) in Pichia pastoris using the glyceraldehydes-3-phosphate dehydrogenase promoter. The maximal level of HDAO expression using the P(GAP) integrant is attained in 13 h and is equal to that obtained using the P(AOX1) integrant in 43 h.In-frame fusion of Saccharomyces cerevisiae alpha-factor secretion signal under a P(GAP) or P(AOX1) results in low-level secretion of active HDAO, which is not of practical use
-
expression of recombinant enzyme, using the CYP1A1 promoter, in murine hepatoma Hepa1c1c7 cells
-
expression of recombinant N-terminally Strep-tagged enzyme in Escherichia coli strain BL21(DE3)
-
expression of Strep-tagged wild-type and mutant enzymes in Escherichia coli strain BL21 (DE3), induction by DL-Ala
-
expression of wild-type enzyme and mutants F54Y in Escherichia coli BL21(DE3)
functional enzyme overexpression in Escherichia coli strain BL21(DE3), method optimization and upscaling, detailed overview
-
gene Y69Ap, expression of DAO in Escherichia coli
-
genotyping
genotyping of the enzyme in healthy and schizophrenic individuals, association between dysbindin and DAO single nucleotide polymorphisms and the positive and negative syndrome scale, PANSS, overview. Significant association between the dysbindin SNP rs3213207 and severity of both negative symptoms and total symptom load, as well as between the DAO SNP rs2070587 and total symptom score and severity of anxiety and depression
-
overexpression in Candida boidinii strain aod1 DELTA
-
overexpression in Escherichia coli
quantitative real-time PCR expression analysis in different cells
-
rat C6 glial cell overexpressing mouse D-amino acid oxidase
-
real-time quantitative PCR expression analysis
-
recombinant enzyme expression in Escherichia coli
-
recombinant enzyme expression in Escherichia coli strain BL21(DE3), optimization of fermentation and cryopreservation of the recombinant Escherichia coli cells, detailed overview
-
recombinant expression in rat C6 cells
-
recombinant expression of the enzyme
-
simple and rapid genotyping of D-amino acid oxidase gene recognizing a crucial variant in the ddY strain using microchip electrophoresis, method development, overview. Verification of the genotyping method by the crossbreeding of DAO+/+ and DAO-/- mice
-
stable expression in Pichia pastoris with 7fold enhancement of the intracellular level of oxidase activity, expression optimization by codon redesign as well as efficient subcellular targeting of the enzyme to peroxisomes
the gene encoding the enzyme is located on chromosome 12q24
-
the His-tagged enzyme is expressed in Escherichia coli
two cDNA genes were connected by a hexanucleotide linker and heterologously expressed in Escherichia coli to produce the corresponding double DAO with two subunits fused into a single polypeptide