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84.08
L-Asn
-
pH 8.5, 25°C
47.49
L-Asp
-
pH 8.5, 25°C
430.4
L-Gln
-
pH 8.5, 25°C
483.1
L-His
-
pH 8.5, 25°C
66.7
L-Ile
-
pH 8.5, 25°C
2.6 - 27.8
L-leucine methyl ester
0.1
L-Orn
-
at pH 8.5 and 30°C
0.008 - 3483
L-phenylalanine
0.16
L-phenylalanine methyl ester
-
at pH 8.5 and 30°C
7.75 - 9.62
L-propargylglycine
additional information
additional information
-
mol amino acid/min x mol of enzyme bound FAD: 11000 L-arginine, 280 L-phenylalanine, 40 L-valine, 600 L-leucine
-
0.052 - 2.1
L-alanine
wild-type enzyme
0.057 - 0.65
L-alanine
recombinant enzyme, expressed in and Streptomyces lividans, 30°C, pH 7.6
0.13
L-Arg
-
at pH 8.5 and 30°C
228.8
L-Arg
-
pH 8.5, 25°C
0.1
L-arginine
mutant D238A, pH 7, 30°C
0.1
L-arginine
mutant D238F, pH 7, 30°C
0.41
L-arginine
wild-type, pH 7, 30°C
17.6
L-Glu
-
pH and temperature not specified in the publication
790.5
L-Glu
-
pH 8.5, 25°C
2.56
L-glutamine
untreated recombinant protein, pH 7, 30°C
38.8
L-glutamine
acid-activited recombinant protein, pH 7, 30°C
6.33
L-isoleucine
-
-
3421
L-isoleucine
pH not specified in the publication, temperature not specified in the publication
0.13
L-Leu
-
at pH 8.5 and 30°C
1.8
L-Leu
-
pH and temperature not specified in the publication
3.3
L-Leu
-
pH and temperature not specified in the publication
24.4
L-Leu
-
at pH 7.8 and 50°C
47.98
L-Leu
-
pH and temperature not specified in the publication
48.22
L-Leu
-
pH and temperature not specified in the publication
52
L-Leu
-
pH and temperature not specified in the publication
75.16
L-Leu
-
pH and temperature not specified in the publication
96.2
L-Leu
-
pH and temperature not specified in the publication
1025
L-Leu
-
pH 8.5, 25°C
1025.05
L-Leu
-
pH and temperature not specified in the publication
1.8
L-leucine
-
in 50 mM Tris-HCl buffer pH 8.0, at 37°C
2.32
L-leucine
untreated recombinant protein, pH 7, 30°C
12
L-leucine
-
pH 8, 37°C
45.3
L-leucine
acid-activited recombinant protein, pH 7, 30°C
52
L-leucine
-
pH 8.5, 25°C
5965
L-leucine
pH not specified in the publication, temperature not specified in the publication
2.6
L-leucine methyl ester
untreated recombinant protein, pH 7, 30°C
27.8
L-leucine methyl ester
acid-activited recombinant protein, pH 7, 30°C
0.04
L-Lys
-
at pH 7.0 and 30°C
0.09
L-Lys
-
at pH 8.5 and 30°C
2.6
L-Lys
37°C, isozyme MPLAO1
4.7
L-Lys
37°C, isozyme MPLAO3
318.9
L-Lys
-
pH 8.5, 25°C
0.00085
L-lysine
pH 7, 37°C, EDTA-treated protein
0.0302
L-lysine
pH 7, 37°C, untreated protein
0.17
L-lysine
mutant D238F, pH 7, 30°C
0.5
L-lysine
mutant D238A, pH 7, 30°C
0.8
L-lysine
wild-type, pH 7, 30°C
2.3
L-lysine
mutant D238E, pH 7, 30°C
57.1
L-lysine
-
pH 4.0, 37°C
0.039
L-Met
-
at pH 7.0 and 30°C
0.08
L-Met
-
at pH 8.5 and 30°C
1.65
L-Met
-
pH and temperature not specified in the publication
18.5
L-Met
-
at pH 7.8 and 50°C
24.13
L-Met
-
pH and temperature not specified in the publication
65.6
L-Met
-
pH and temperature not specified in the publication
66.26
L-Met
-
pH and temperature not specified in the publication
74.2
L-Met
-
pH and temperature not specified in the publication
80.3
L-Met
-
pH and temperature not specified in the publication
589.3
L-Met
-
pH 8.5, 25°C
589.93
L-Met
-
pH and temperature not specified in the publication
0.21
L-methionine
-
pH 8.5, 25°C
80.3
L-methionine
-
pH 8.5, 25°C
7284
L-methionine
pH not specified in the publication, temperature not specified in the publication
0.37
L-ornithine
mutant D238A, pH 7, 30°C
0.58
L-ornithine
mutant D238E, pH 7, 30°C
1.5
L-ornithine
wild-type, pH 7, 30°C
0.039
L-Phe
-
at pH 7.0 and 30°C
0.16
L-Phe
-
at pH 8.5 and 30°C
0.72
L-Phe
-
pH and temperature not specified in the publication
17.18
L-Phe
-
pH and temperature not specified in the publication
28.5
L-Phe
-
pH and temperature not specified in the publication
31.4
L-Phe
-
at pH 7.8 and 50°C
48.23
L-Phe
-
pH and temperature not specified in the publication
54.1
L-Phe
-
pH and temperature not specified in the publication
142.6
L-Phe
-
pH 8.5, 25°C
142.62
L-Phe
-
pH and temperature not specified in the publication
0.008
L-phenylalanine
-
at pH 7.0 and 20°C
0.013
L-phenylalanine
-
at pH 7.0 and 30°C
0.02
L-phenylalanine
-
at pH 7.0 and 50°C
0.025
L-phenylalanine
-
at pH 7.0 and 40°C
0.83
L-phenylalanine
untreated recombinant protein, pH 7, 30°C
1.4
L-phenylalanine
-
wild-type, pH 7.0, temperature not specified in the publication
1.67
L-phenylalanine
-
mutant D165K, pH 7.0, temperature not specified in the publication
1.69
L-phenylalanine
-
mutant L336M, pH 7.0, temperature not specified in the publication
1.72
L-phenylalanine
-
mutant D165K/L336M, pH 7.0, temperature not specified in the publication
1.82
L-phenylalanine
-
mutant D165K/S179L/F263V/L336V, pH 7.0, temperature not specified in the publication
1.87
L-phenylalanine
-
mutant F263M, pH 7.0, temperature not specified in the publication
2.16
L-phenylalanine
-
mutant D165K/F263M, pH 7.0, temperature not specified in the publication
2.21
L-phenylalanine
-
mutant F263M/L336M, pH 7.0, temperature not specified in the publication
2.25
L-phenylalanine
-
mutant D165K/F263M/L336M, pH 7.0, temperature not specified in the publication
6.27
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28°C
6.78
L-phenylalanine
-
in 20 mM Na-phosphate, pH 7.5, at 28°C
17.18
L-phenylalanine
-
-
21.9
L-phenylalanine
acid-activited recombinant protein, pH 7, 30°C
48.23
L-phenylalanine
-
-
3483
L-phenylalanine
pH not specified in the publication, temperature not specified in the publication
7.75
L-propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28°C
9.62
L-propargylglycine
-
in 20 mM Na-phosphate, pH 7.5, at 28°C
0.88
L-Trp
-
pH and temperature not specified in the publication
6.58
L-Trp
-
pH and temperature not specified in the publication
18.04
L-Trp
-
pH and temperature not specified in the publication
32.1
L-Trp
-
pH and temperature not specified in the publication
42.65
L-Trp
-
pH and temperature not specified in the publication
98.82
L-Trp
-
pH 8.5, 25°C
98.82
L-Trp
-
pH and temperature not specified in the publication
6.58
L-tryptophan
-
-
42.6
L-tryptophan
-
pH 8.5, 25°C
3720
L-tryptophan
pH not specified in the publication, temperature not specified in the publication
0.036
L-Tyr
-
at pH 7.0 and 30°C
100.6
L-Tyr
-
pH 8.5, 25°C