Any feedback?
Please rate this page
(all_enzymes.php)
(0/150)

BRENDA support

1.4.1.9: leucine dehydrogenase

This is an abbreviated version!
For detailed information about leucine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.9

Reaction

L-leucine
+
H2O
+
NAD+
=
4-methyl-2-oxopentanoate
+
NH3
+
NADH
+
H+

Synonyms

BCD, dehydrogenase, leucine, L-leucine dehydrogenase, L-leucine:NAD+ oxidoreductase, deaminating, LeuDH

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.9 leucine dehydrogenase

KM Value

KM Value on EC 1.4.1.9 - leucine dehydrogenase

Please wait a moment until all data is loaded. This message will disappear when all data is loaded.
KM VALUE [mM]
SUBSTRATE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
4
2-ketoisocaproate
-
pH 9.5, 30°C
19
2-oxo-3,3-dimethylbutanoate
-
-
0.73 - 2.54
2-oxo-3-methylbutanoate
0.9 - 2.2
2-oxo-3-methylpentanoate
13
2-oxo-4-methylselenobutanoate
-
-
0.705 - 22.74
2-oxobutanoate
1.2 - 28
2-Oxohexanoate
0.88 - 25
2-Oxoisohexanoate
0.4 - 2.4
2-oxopentanoate
2.6 - 217.7
3,3-dimethyl-2-oxobutanoate
2.41
3-acetylpyridine-deamino-NAD+
-
-
0.77
3-acetylpyridine-NAD+
-
-
1.4 - 4.4
3-methyl-2-oxobutanoate
0.38
3-methyl-2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
2.1
3-methylthio-2-oxobutanoate
-
-
6.7 - 8.1
4-(methylsulfanyl)-2-oxobutanoate
2.7
4-methyl-2-oxo-5,5,5-trifluoropentanoate
-
-
0.31 - 140
4-methyl-2-oxopentanoate
3.8 - 69
alpha-keto-beta-methylvalerate
0.88 - 30
alpha-keto-isocaproate
0.75
DL-methionine
pH 9, 30°C
1.5 - 22
L-2-aminobutanoate
0.84
L-arginine
pH 9, 30°C
0.4 - 33
L-Ile
0.69 - 130
L-Leu
0.1 - 1.4
L-leucine
23 - 25
L-Met
1.27 - 24
L-norleucine
0.98 - 70
L-norvaline
31 - 66
L-Phe
0.64
L-proline
pH 9, 30°C
1.8
L-tert-Leu
-
-
5.05
L-tert-leucine
Roseibium aggregatum
-
wild-type, pH 8, 30°C
0.48
L-tyrosine
pH 9, 30°C
0.71 - 25
L-Val
0.015 - 17
NAD+
0.017 - 0.624
NADH
2.5 - 2.8
NADP+
220 - 330
NH3
13 - 750
NH4+
0.75 - 9.38
oxo(phenyl)acetate
-
7.1 - 9.9
phenylpyruvate
0.09 - 60.8
trimethylpyruvate