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4
2-ketoisocaproate
-
pH 9.5, 30°C
19
2-oxo-3,3-dimethylbutanoate
-
-
0.73 - 2.54
2-oxo-3-methylbutanoate
0.9 - 2.2
2-oxo-3-methylpentanoate
13
2-oxo-4-methylselenobutanoate
-
-
0.705 - 22.74
2-oxobutanoate
0.88 - 25
2-Oxoisohexanoate
0.4 - 2.4
2-oxopentanoate
2.6 - 217.7
3,3-dimethyl-2-oxobutanoate
2.41
3-acetylpyridine-deamino-NAD+
-
-
0.77
3-acetylpyridine-NAD+
-
-
1.4 - 4.4
3-methyl-2-oxobutanoate
0.38
3-methyl-2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
2.1
3-methylthio-2-oxobutanoate
-
-
6.7 - 8.1
4-(methylsulfanyl)-2-oxobutanoate
2.7
4-methyl-2-oxo-5,5,5-trifluoropentanoate
-
-
0.31 - 140
4-methyl-2-oxopentanoate
3.8 - 69
alpha-keto-beta-methylvalerate
0.88 - 30
alpha-keto-isocaproate
0.75
DL-methionine
pH 9, 30°C
1.5 - 22
L-2-aminobutanoate
0.84
L-arginine
pH 9, 30°C
0.64
L-proline
pH 9, 30°C
5.05
L-tert-leucine
Roseibium aggregatum
-
wild-type, pH 8, 30°C
0.48
L-tyrosine
pH 9, 30°C
0.75 - 9.38
oxo(phenyl)acetate
-
0.09 - 60.8
trimethylpyruvate
0.73
2-oxo-3-methylbutanoate
mutant E116V, pH 9.5, 35°C
0.93
2-oxo-3-methylbutanoate
mutant T45M/E116V, pH 9.5, 35°C
2.47
2-oxo-3-methylbutanoate
wild-type, pH 9.5, 35°C
2.54
2-oxo-3-methylbutanoate
mutant T45M, pH 9.5, 35°C
0.9
2-oxo-3-methylpentanoate
-
-
0.9
2-oxo-3-methylpentanoate
-
-
1.9
2-oxo-3-methylpentanoate
-
-
2.2
2-oxo-3-methylpentanoate
-
-
0.705
2-oxobutanoate
mutant E116V, pH 9.5, 35°C
1.7
2-oxobutanoate
mutant T45M, pH 9.5, 35°C
1.89
2-oxobutanoate
wild-type, pH 9.5, 35°C
2.65
2-oxobutanoate
mutant T45M/E116V, pH 9.5, 35°C
13.5
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
13.53
2-oxobutanoate
-
mutant Q358N, pH 7.5, 37°C
16.8
2-oxobutanoate
-
mutant M347G/Q358N, pH 7.5, 37°C
21.3
2-oxobutanoate
-
mutant M374G, pH 7.5, 37°C
21.33
2-oxobutanoate
-
mutant M347G, pH 7.5, 37°C
22.7
2-oxobutanoate
-
wild-type, pH 7.5, 37°C
22.74
2-oxobutanoate
-
wild-type, pH 7.5, 37°C
1.2
2-Oxohexanoate
-
-
2
2-Oxohexanoate
-
mutant enzyme A113A
4.7
2-Oxohexanoate
-
wild-type enzyme
28
2-Oxohexanoate
-
mutant enzyme A113A/V291L
0.88
2-Oxoisohexanoate
-
wild-type enzyme
0.99
2-Oxoisohexanoate
-
mutant enzyme K80R
5.5
2-Oxoisohexanoate
-
mutant enzyme G77A
5.6
2-Oxoisohexanoate
-
mutant enzyme G79A
7.7
2-Oxoisohexanoate
-
mutant enzyme G78A
9.8
2-Oxoisohexanoate
-
mutant enzyme K80A
25
2-Oxoisohexanoate
-
mutant enzyme K80Q
0.4
2-oxopentanoate
-
-
0.41
2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
1.08
2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
1.14
2-oxopentanoate
mutant E116V, pH 9.5, 35°C
1.37
2-oxopentanoate
-
pH 8, 50°C
1.43
2-oxopentanoate
wild-type, pH 9.5, 35°C
1.99
2-oxopentanoate
mutant T45M, pH 9.5, 35°C
2.6
3,3-dimethyl-2-oxobutanoate
mutant A43V/D124E , pH 10.0, 30°C
12
3,3-dimethyl-2-oxobutanoate
wild-type, pH 10.0, 30°C
16.8
3,3-dimethyl-2-oxobutanoate
-
pH 9.5, 30°C
100
3,3-dimethyl-2-oxobutanoate
-
pH 7.0, temperature not specified in the publication
153.6
3,3-dimethyl-2-oxobutanoate
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
217.7
3,3-dimethyl-2-oxobutanoate
-
His-tagged enzyme, pH 9.0, 25°C
1.4
3-methyl-2-oxobutanoate
-
-
2.1
3-methyl-2-oxobutanoate
-
-
2.2
3-methyl-2-oxobutanoate
-
-
2.2
3-methyl-2-oxobutanoate
-
-
2.4
3-methyl-2-oxobutanoate
-
-
3.3
3-methyl-2-oxobutanoate
-
-
3.8
3-methyl-2-oxobutanoate
-
-
4
3-methyl-2-oxobutanoate
-
-
4.4
3-methyl-2-oxobutanoate
-
-
6.7
4-(methylsulfanyl)-2-oxobutanoate
-
-
8.1
4-(methylsulfanyl)-2-oxobutanoate
-
-
0.31
4-methyl-2-oxopentanoate
-
-
0.38
4-methyl-2-oxopentanoate
mutant E116V, pH 9.5, 35°C
0.45
4-methyl-2-oxopentanoate
-
-
0.6
4-methyl-2-oxopentanoate
-
-
0.63
4-methyl-2-oxopentanoate
-
-
0.7
4-methyl-2-oxopentanoate
-
-
0.8
4-methyl-2-oxopentanoate
-
-
0.89
4-methyl-2-oxopentanoate
mutant T45M/E116V, pH 9.5, 35°C
1
4-methyl-2-oxopentanoate
-
-
1.2
4-methyl-2-oxopentanoate
-
mutant enzyme K68R
1.41
4-methyl-2-oxopentanoate
wild-type, pH 9.5, 35°C
1.55
4-methyl-2-oxopentanoate
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
1.72
4-methyl-2-oxopentanoate
mutant T45M, pH 9.5, 35°C
140
4-methyl-2-oxopentanoate
-
mutant enzyme K68A
3.8
alpha-keto-beta-methylvalerate
-
wild-type enzyme
9.4
alpha-keto-beta-methylvalerate
-
mutant enzyme A113A
69
alpha-keto-beta-methylvalerate
-
mutant enzyme A113A/V291L
0.88
alpha-keto-isocaproate
-
wild-type enzyme
1.7
alpha-keto-isocaproate
-
mutant enzyme A113A
30
alpha-keto-isocaproate
-
mutant enzyme A113A/V291L
1.5
L-2-aminobutanoate
-
-
8.9
L-2-aminobutanoate
-
-
10
L-2-aminobutanoate
-
-
22
L-2-aminobutanoate
-
-
0.4
L-Ile
-
-
0.66
L-Ile
-
pH 9.5, 30°C, in presence of 4 M NaCL
0.78
L-Ile
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
2.4
L-Ile
-
wild-type enzyme
7.8
L-Ile
-
mutant enzyme A113A
33
L-Ile
-
mutant enzyme A113A/V291L
0.69
L-Leu
-
pH 9.5, 30°C
1.12
L-Leu
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
1.4
L-Leu
-
mutant enzyme A113A
3.2
L-Leu
-
mutant enzyme K68R
3.3
L-Leu
-
native enzyme
3.5
L-Leu
-
mutant enzyme G77A
3.6
L-Leu
-
mutant enzyme G79A
3.7
L-Leu
-
mutant enzyme K80A
4.3
L-Leu
-
mutant enzyme G78A
5.1
L-Leu
-
wild-type enzyme
11
L-Leu
-
mutant enzyme LeuDEL4
35
L-Leu
-
mutant enzyme A113A/V291L
130
L-Leu
-
mutant enzyme K68A
0.1
L-leucine
-
pH 9.5, 30°C
0.33
L-leucine
pH 9, 30°C
0.65
L-leucine
-
pH 10.5, 20°C
0.8
L-leucine
-
pH 9.5, 30°C
0.88
L-leucine
pH 8.5, 30°C
1.2
L-leucine
-
pH not specified in the publication, temperature not specified in the publication
1.4
L-leucine
-
pH 10.5, 25°C
23
L-Met
-
-
1.27
L-norleucine
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
4.1
L-norleucine
-
mutant enzyme A113A
4.4
L-norleucine
-
wild-type enzyme
24
L-norleucine
-
mutant enzyme A113A/V291L
0.98
L-norvaline
-
in 100 mM glycine-KCl-KOH buffer (pH 10), at 25°C
7.8
L-norvaline
-
wild-type enzyme
27
L-norvaline
-
mutant enzyme A113A
70
L-norvaline
-
mutant enzyme A113A/V291L
31
L-Phe
-
mutant enzyme A113A
66
L-Phe
-
mutant enzyme A113A/V291L
0.71
L-Val
-
pH 9.5, 30°C, in presence of 4 M NaCL
3.8
L-Val
-
native enzyme
25
L-Val
-
mutant enzyme LeuDEL4
0.015
NAD+
-
pH 10.5, 20°C
0.034
NAD+
-
mutant enzyme K80Q
0.039
NAD+
-
mutant enzyme G77A
0.055
NAD+
-
mutant enzyme K68R
0.059
NAD+
-
mutant enzyme G78A
0.063
NAD+
-
wild-type enzyme
0.071
NAD+
-
mutant enzyme G79A
0.074
NAD+
-
mutant enzyme K80R
0.076
NAD+
-
mutant enzyme A113A
0.078
NAD+
Roseibium aggregatum
-
wild-type, pH 8, 30°C
0.094
NAD+
-
pH 10.5, 25°C
0.11
NAD+
-
mutant enzyme K68A
0.14
NAD+
-
mutant enzyme K80A
0.28
NAD+
-
mutant enzyme A113A/V291L
0.48
NAD+
-
native enzyme
1.25
NAD+
-
mutant enzyme LeuDEL4
2.8
NAD+
-
mutant enzyme K80R
17
NAD+
-
mutant enzyme K80Q
0.017
NADH
-
mutant enzyme A113A/V291L
0.02
NADH
Roseibium aggregatum
-
mutant D153N/H191N, pH 8, 30°C
0.021
NADH
-
mutant enzyme K68R
0.022
NADH
-
mutant enzyme K80A
0.0223
NADH
pH 10.0, 30°C, fusion enzyme F-S1_L
0.0279
NADH
pH 10.0, 30°C, fusion enzyme F-R3-L
0.0349
NADH
pH 10.0, 30°C, fusion enzyme F-S3-L
0.035
NADH
-
wild-type enzyme
0.037
NADH
-
mutant enzyme K80R
0.038
NADH
-
mutant enzyme G77A
0.04
NADH
mutant A43V/D124E , pH 10.0, 30°C
0.04
NADH
Roseibium aggregatum
-
mutant D153N, pH 8, 30°C
0.0415
NADH
pH 10.0, 30°C, fusion enzyme F-DL-L
0.042
NADH
-
mutant enzyme A113A
0.0436
NADH
pH 10.0, 30°C, fusion enzyme F-R2-L
0.045
NADH
-
mutant enzyme K80Q
0.05
NADH
Roseibium aggregatum
-
mutant P130V, pH 8, 30°C
0.051
NADH
pH 10.0, 30°C, fusion enzyme F-R1-L
0.053
NADH
-
mutant enzyme K68A
0.054
NADH
-
mutant enzyme G78A
0.055
NADH
-
mutant enzyme G79A
0.0605
NADH
pH 10.0, 30°C, wild-type enzyme
0.061
NADH
-
enzyme coupled to dockerin from Clostridium thermocellum, pH 9.0, 25°C
0.07
NADH
mutant E116V, pH 9.5, 35°C
0.07
NADH
Roseibium aggregatum
-
mutant H191N, pH 8, 30°C
0.077
NADH
-
pH 9.5, 30°C
0.09
NADH
mutant T45M/E116V, pH 9.5, 35°C
0.1
NADH
-
pH 7.0, temperature not specified in the publication
0.1
NADH
mutant E24V/E116V, pH 8.5, 30°C
0.151
NADH
pH 10.0, 30°C, fusion enzyme F-S2_L
0.154
NADH
mutant E24A/D126E, pH 8.5, 30°C
0.17
NADH
wild-type, pH 9.5, 35°C
0.2
NADH
wild-type, pH 10.0, 30°C
0.22
NADH
mutant T45M, pH 9.5, 35°C
0.248
NADH
-
His-tagged enzyme, pH 9.0, 25°C
0.25
NADH
mutant E116V, pH 8.5, 30°C
0.27
NADH
mutant D126E, pH 8.5, 30°C
0.28
NADH
wild-type, pH 8.5, 30°C
0.43
NADH
Roseibium aggregatum
-
wild-type, pH 8, 30°C
2.5
NADP+
-
mutant D203A/I204R/D210R
2.8
NADP+
-
mutant enzyme D203A/I204R
220
NH3
-
pH 9.5, 30°C
13
NH4+
-
-
75
NH4+
-
wild-type enzyme
210
NH4+
-
mutant enzyme G77A
290
NH4+
-
mutant enzyme G79A
750
NH4+
-
mutant enzyme G78A
0.75
oxo(phenyl)acetate
mutant E116V, pH 9.5, 35°C
-
1.12
oxo(phenyl)acetate
mutant T45M/E116V, pH 9.5, 35°C
-
1.99
oxo(phenyl)acetate
mutant T45M, pH 9.5, 35°C
-
9.38
oxo(phenyl)acetate
wild-type, pH 9.5, 35°C
-
7.1
phenylpyruvate
-
mutant enzyme A113A
9.9
phenylpyruvate
-
mutant enzyme A113A/V291L
0.09
trimethylpyruvate
Roseibium aggregatum
-
mutant H191N, pH 8, 30°C
0.096
trimethylpyruvate
Roseibium aggregatum
-
wild-type, pH 8, 30°C
0.1
trimethylpyruvate
Roseibium aggregatum
-
mutant D153N/H191N, pH 8, 30°C
0.12
trimethylpyruvate
Roseibium aggregatum
-
mutant D153N, pH 8, 30°C
0.3
trimethylpyruvate
Roseibium aggregatum
-
mutant P130V, pH 8, 30°C
1.15
trimethylpyruvate
mutant T45M, pH 9.5, 35°C
1.71
trimethylpyruvate
mutant E116V, pH 9.5, 35°C
2.65
trimethylpyruvate
mutant T45M/E116V, pH 9.5, 35°C
3.08
trimethylpyruvate
-
pH 9.5, 30°C
3.7 - 5
trimethylpyruvate
wild-type, pH 9.5, 35°C
5.96
trimethylpyruvate
pH 8.5, 30°C
7.9
trimethylpyruvate
mutant E24V/E116V, pH 8.5, 30°C
10.7
trimethylpyruvate
mutant E116V, pH 8.5, 30°C
17.6
trimethylpyruvate
mutant E24A/D126E, pH 8.5, 30°C
23.2
trimethylpyruvate
mutant D126E, pH 8.5, 30°C
60.8
trimethylpyruvate
wild-type, pH 8.5, 30°C