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1.4.1.4: glutamate dehydrogenase (NADP+)

This is an abbreviated version!
For detailed information about glutamate dehydrogenase (NADP+), go to the full flat file.

Word Map on EC 1.4.1.4

Reaction

L-glutamate
+
H2O
+
NADP+
=
2-oxoglutarate
+
NH3
+
NADPH
+
H+

Synonyms

APE1386, BpNADPGDH I, BpNADPGDH II, dehydrogenase, glutamate (nicotinamide adenine dinucleotide phosphate), EcGDH, GDH, GDH1, Gdh1p, GDH2, GDH3, Gdh3p, GDH4, GdhA, GDHB, GDHC, GDHI, GDHI', GDHII, GDHP, GluDH, glutamate dehydrogenase, glutamate dehydrogenase 1, glutamate dehydrogenase 2, glutamate dehydrogenase 3, glutamic acid dehydrogenase, glutamic dehydrogenase, GW612_14215, H-form specific BpNADPGDH II, hGDH, hyphal-form specific BpNADPGDH II, L-glutamate dehydrogenase, NAD(P)H-dependent glutamate dehydrogenase, NADP(+)-dependent glutamate dehydrogenase, NADP(H)-dependent glutamate dehydrogenase, NADP(H)-GDH, NADP(H)-specific glutamate dehydrogenase, NADP+ - dependant-glutamate dehydrogenase, NADP+-dependent GDH, NADP+-dependent glutamate dehydrogenase, NADP+-Gdh, NADP-dependent GDH, NADP-dependent GluDH, NADP-dependent glutamate dehydrogenase, NADP-dependent glutamate dehydrogenase 1, NADP-dependent glutamate dehydrogenase 2, NADP-GDH, NADP-GDH 1, NADP-GDH 2, NADP-glutamate dehydrogenase, NADP-linked glutamate dehydrogenase, NADP-specific glutamate dehydrogenase, NADP-specific L-glutamate dehydrogenase, NADP-ylGdh1p, NADPH-dependent GDH, NADPH-dependent glutamate dehydrogenase, OsGDH4, Pcal_1606, RocG, TrGDH, Y-form specific BpNADPGDH I, YALI0F17820g, yeast-form specific BpNADPGDH I, ylGDH1

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.4 glutamate dehydrogenase (NADP+)

Cloned

Cloned on EC 1.4.1.4 - glutamate dehydrogenase (NADP+)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli strain TG1
-
expression in Escherichia coli
expression in Escherichia coli BL21
expression in Escherichia coli DH5alpha under the control of a highly constitutive expresion promoter in a pHCE system
-
expression in Escherichia coli JM109
-
expression in Escherichia coli, hybrid proteins containing the Pyrococcus furiosus glutamate and the Clostridium difficile cofactor binding domain with reduced substrate binding affinity
expression in GDH-deficient Saccharomyces cerevisiae
full-length cDNA encoding alpha-subunit, beta-subunit and a truncated subunit missing 40 amino acids at their N-terminus, DELTA40N, expression in Escherichia coli
-
GDH1, cloning and expression of wild-type and mutant enzymes in Escherichia coli, recombinant expression of GFP-tagged Gdh1 in Saccharomyces cerevisiae
gene gdh, quantitative real-time PCR expression analysis in different cheese cultures
gene GDH1, DNA and amino acid sequence determination and analysis, overexpression as His6-tagged protein in Escherichia coli strain KRX
gene gdh1, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, expression of BpNADPGDH I does not show any change in morphology of Candida glabrata
gene GDH1, expression as His6-tagged enzyme in Escherichia coli strain M15, method optimization
gene gdh1, recombinant expression of His-tagged isozyme BpNADPGDH I in Escherichia coli strain BL21(DE3)
gene gdh3, DNA and amino acid sequence determination and analysis, phylogenetic analysis, quantitative RT-PCR enzyme expression analysis, recombinant expression in the monomorphic mutant Y-5 of Benjaminiella poitrasii and in human-pathogenic, non-dimorphic Candida glabrata, the transformation with hyphal form specific BpNADPGDH II gene leads to germ tube formation in Candida glabrata under normal growth conditions
gene gdh3, recombinant expression of His-tagged isozyme BpNADPGDH II in Escherichia coli strain BL21(DE3)
gene gdhA, phylogenetic analysis, expression of His6-tagged GdhA in Escherichia coli, ectopic expression in the cytoplasm of transgenic Oryza sativa plants. The introduced gdhA, together with the endogenous glutamine synthetase, directly assimilates NH4 + absorbed from the roots. NADP(H)-GDH activities in the gdhA-transgenic lines are markedly higher than those in a control line
-
gene gdhA, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain BL21(DE3)
gene msmeg_5442, semi-quantitative real-time PCR expression analysis
gene pcal_1606, DNA and amino acid sequence determination and analysis, phylogenetic analysis, expression of N-terminally Met-Ala-Ser-tagged and C-terminally His6-tagged enzyme in Eschericia coli strain Rosetta(DE3)
gene YALI0F17820g, cloned from strain INAG34815, sequence comparisons, recombinant expression in gdh-null Saccharomyces cerevisiae, recombinant overexpression in Yarrowia lipolytica strain under control of the constitutive TEF promoter using the JMP62 expression vectors. GDH activities in Yarrowia lipolytica are differentially expressed, overview
genes GDH1 and GDH3, recombinant expression of FLAG-tagged wild-type and mutant GDH1 and GDH3 in yeast cells
-
mutant GDH transformed into competent Escherichia coli PA340 cells, which are glutamate synthase and dehydrogenase-deficient
-
overexpression of the gdhA gene, which encodes NADP-GDH, can modulate nitrogen metabolism in Lycopersicon esculentum, with resultsnt increase in levels od some free amino acids, in particular glutamate, in tomato fruits
-
overexpression with the NICE system in Lactococcus lactis TIL46 induced with increasing concentrations of nisin, pGdh442 transferred by electroporation (TIL504) or by conjugation (TIL507) in Lactococcus lactis MG1363 or in its derivative TIL193 strain containing pGhost8, appears to be stable after several subcultures of the strains without selection pressure, in the presence of plasmid pLP712, pGdh442 is unstable
-
sequence comparisons, expression of wild-type and mutant enzymes
TrGDH, DNA and amino acid sequence determination and analysis, phylogenetic analysis, recombinant FLAG-tagged enzyme expression in Oryza sativa cv. Kitaake under control of the 35S CaMV 35S promoter, semiquantitative RT-PCR and quantitative real-time PCR enzyme expression analysis
-