1.4.1.20: phenylalanine dehydrogenase This is an abbreviated version! For detailed information about phenylalanine dehydrogenase, go to the full flat file .
Reaction
L-phenylalanine +
H2O +
NAD+ =
phenylpyruvate +
NH3 +
NADH +
H+
Synonyms dehydrogenase, phenylalanine, L-PheDH, L-phenylalanine dehydrogenase, L-phenylalanine:NAD+ oxidoreductase, deaminating, PDH, PHD, PheDH, phenylalanine dehydrogenase, recombinant PheDH, recombinant phenylalanine dehydrogenase
ECTree
Inhibitors
Inhibitors on EC 1.4.1.20 - phenylalanine dehydrogenase
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3-Phenylpropionate
-
competitive with L-Phe
4-hydroxyphenylethylamine
-
competitive with L-Phe
5,5'-dithio-bis(2-nitrobenzoic acid)
acetonitrile
in the presence of acetonitrile at 10% (v/v), the enzyme retains about 60% of the original activity. However, the enzyme exhibits nearly no activity in the presence of acetonitrile at 30% (v/v)
alpha-methyl-DL-phenylalanine
-
-
D-Val
-
10 mM, 11% inhibition
Isopropanol
in the presence of isopropanol at 10% (v/v), the enzyme retains about 30% of the original activity. However, the enzyme exhibits nearly no activity in the presence of isopropanol at 30% (v/v)
L-Cys
-
10 mM, 41% inhibition
L-Ile
-
10 mM, 75% inhibition
L-phenylglycine
-
competitive with L-Phe
L-Val
-
10 mM, 23% inhibition
methyl acetyl phosphate
-
irreversible inactivation, simultaneous addition of substrate and coenzyme markedly protect from inactivation, the reagent can acetylate Lys69 and Lys81
NEM
-
1 mM: 79% inhibition
additional information
the enzyme exhibits nearly no change on enzyme activity in the presence of glycol, ethanol and methanol at 10% (v/v)
-
5,5'-dithio-bis(2-nitrobenzoic acid)
-
0.17 mM: 26% inhibition
5,5'-dithio-bis(2-nitrobenzoic acid)
-
0.17 mM, complete inhibition
AgNO3
-
1 mM, complete inhibition
AgNO3
-
1 mM, complete inhibition
D-Leu
-
10 mM, 17% inhibition
D-Leu
-
10 mM, 31% inhibition
D-Met
-
10 mM, 63% inhibition
D-Met
-
10 mM, 25% inhibition
D-norleucine
-
10 mM, 52% inhibition
D-norleucine
-
10 mM, 34% inhibition
D-Phe
-
competitive with L-Phe
D-Phe
-
10 mM, 89% inhibition
D-Phe
-
10 mM, 77% inhibition
D-Trp
-
5 mM, 83% inhibition
D-Trp
-
5 mM, 65% inhibition
D-Tyr
-
competitive with L-Phe
D-Tyr
-
1 mM, 29% inhibition
HgCl2
-
0.01 mM, complete inhibition
HgCl2
-
0.01 mM, 62% inhibition
HgCl2
-
1.0 mM, 97% inhibition
HgCl2
-
0.01 M, complete inhibition
L-Leu
-
10 mM, 58% inhibition
L-Leu
-
10 mM, 39% inhibition
L-Phe
-
product inhibition
NAD+
-
product inhibition
PCMB
-
0.001 mM, complete inhibition
PCMB
-
0.02 mM, complete inhibition
PCMB
-
1.0 mM, 98% loss of activity
PCMB
-
0.02 mM, complete inhibition
Phenylethylamine
-
competitive with L-Phe
trans-cinnamate
-
competitive with L-Phe