1.4.1.14: glutamate synthase (NADH)
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For detailed information about glutamate synthase (NADH), go to the full flat file.
Reaction
Synonyms
gltA, GltA2, gltB, glutamate (reduced nicotinamide adenine dinucleotide) synthase, glutamate synthase, glutamate synthase (NADH), glutamate synthase (NADH-dependent), glutamate synthetase, glutamine 2-oxoglutarate aminotransferase, GOGAT, GOGAT1, GOGAT2, L-glutamate synthase (NADH), L-glutamate synthetase, NADH glutamate synthase, NADH-dependent glutamate synthase, NADH-dependent glutamine-2-oxoglutarate aminotransferase, NADH-GltS, NADH-glutamate synthase, NADH-GOGAT, NADH-GOGAT I, NADH-GOGAT II, NADH-GOGAT1, NADH-GOGAT2, NADH: GOGAT, Os01g0681900, Os05g0555600, synthase, glutamate (reduced nicotinamide adenine dinucleotide)
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Engineering
Engineering on EC 1.4.1.14 - glutamate synthase (NADH)
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additional information
construction of the inactive knock-out T-DNA insertion mutant glt1-T, insertion between D315 and G316 of exon 9, mutant plants are specifically defective in growth and glutamate biosynthesis when photorespiration is repressed by 1% CO2, overview
additional information
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GOGAT mutant 4B8 is found to grow onto nitrogen-free medium supplemented with 0.5% glutamate or 0.5% glutamine but not 5 mM NH4Cl, confirming the role of this enzyme in the assimilation of either ammonium derived from fixed and non-fixed nitrogen sources
additional information
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GOGAT-deficient mutants show a growth defect under low NH4 conditions
additional information
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construction of 4 transgenic alfalfa lines, i.e. GA89, GA87, GA88, and GA82, tranformed with an antisense enzyme cDNA fragment under control of the soybean leghemoglobin promotor lbc3, mutants show reduced enzyme activity and content in the nodules, altered chlorotic phenotypes, reduced fresh weight, and lower N-content compared to wild-type plants, overview
additional information
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three en(am)-2 mutants, lacking enzyme activity, no growth on ammonia-limited conditions
additional information
suppression of the enzyme leads to significantly increased 2-oxoglutarate levels in leaves in most co-suppressed lines compared with the wild-type, in the leaf, the 2-oxoglutarate precursor, isocitrate, shows the same trend. The contents of oxaloacetate, malate, and pyruvate are also increased in some, but not all, co-suppressed lines. The mutants show altered chlorophyll contents, phenotypes, overview
additional information
suppression of the enzyme leads to significantly increased 2-oxoglutarate levels in leaves in most co-suppressed lines compared with the wild-type, in the leaf, the 2-oxoglutarate precursor, isocitrate, shows the same trend. The contents of oxaloacetate, malate, and pyruvate are also increased in some, but not all, co-suppressed lines. The mutants show altered chlorophyll contents, phenotypes, overview
additional information
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GOGAT-deficient mutants show a growth defect under low NH4 conditions