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1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
+
NAD+
=
pyruvate
+
NH3
+
NADH
+
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

pH Range

pH Range on EC 1.4.1.1 - alanine dehydrogenase

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pH RANGE
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
3 - 11
purified recombinant AsAlaDH lost activity rapidly at pH 11.0 or higher, while retaining more than 50% activity at pH 9.5. It shows less than 10% activity at neutral pH 7.0 and almost no activity at pH 3.0-6.5
5 - 11
activity range
6 - 8
-
pH 6.0: about 30% of maximal activity, pH 8.5: about 25% of maximal activity, reductive amination
6.1 - 8.3
-
pH 6.1: about 60% of maximal activity, pH 8.3: about 60% of maximal activity, reductive amination
6.2 - 8
-
pH 6.2: about 50% of maximal activity, pH 8.0: about 70% of maximal activity, oxidative deamination
6.5 - 9
-
pH 6.5: about 30% of activity maximum, pH 9.0: about 55% of maximal activity, reductive amination
6.8 - 8.8
-
pH 6.8: about 50% of maximal activity, pH 8.8: about 60% of maximal activity, reductive amination
7 - 10
-
pH 7.0: about 50% of maximal activity, pH 10.0: about 50% of maximal activity, oxidative deamination of L-Ala
7 - 10.8
-
pH 7.0: 13% of maximal activity, pH 10.8: 52% of maximal activity
7.2 - 10
-
pH 7.2: about 10% of activity maximum, pH 10.0: about 30% of activity maximum, reductive amination
7.2 - 9.2
-
pH 7.2: about 40% of maximal activity, pH 9.2: 40% of maximal activity, reductive amination of pyruvate
7.8 - 10
-
pH 7.8: about 50% of maximal activity, pH 10.0: about 65% of maximal activity, oxidative deamination of L-Ala
7.8 - 10.2
-
pH 7.8: 54% of activity maximum, pH 10.2: 72% of activity maximum, reductive amination of pyruvate
7.8 - 9.4
-
pH 7.8: about 50% of activity maximum, pH 9.4: about 90% of activity maximum, reductive amination
8 - 10.5
-
pH 8.0: about 60% of activity maximum, pH 10.5: about 80% of activity maximum, oxidative deamination
8 - 11
8.5 - 10.6
-
pH 8.5: about 60% of maximal activity, pH 10.6: about 40% of maximal activity, oxidative deamination
8.5 - 11.5
-
pH 8.5: about 35% of maximal activity, pH 11.5: about 20% of maximal activity, oxidative deamination
9 - 11.5
Alkalihalophilus pseudofirmus
over 50% of maximal activity within this range wild-type enzyme
9.3 - 11.8
-
pH 9.3: about 55% of activity maximum, pH 11.8: about 63% of activity maximum, oxidative deamination
additional information