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1.4.1.1: alanine dehydrogenase

This is an abbreviated version!
For detailed information about alanine dehydrogenase, go to the full flat file.

Word Map on EC 1.4.1.1

Reaction

L-alanine
+
H2O
 +
NAD+
=
pyruvate
 +
NH3
 +
NADH
 +
H+

Synonyms

(S)alanine dehydrogenase, (S)alanine:NAD oxidoreductase, 40 kDa antigen, ADH, AF1665, ALADH, alanine dehydrogenase, alanine oxidative deaminase, alanine oxidoreductase, ALD, AldA, alpha-alanine dehydrogenase, ApalaDH, AsAlaDH, CQA66_00465, dehydrogenase, alanine, glyoxylate reductive aminase, GxrA, HAADH1, HAADH2, L-Ala dehydrogenase, L-AlaDH, L-AlaDH-Bs, L-alanine dehydrogenase, MtbALD, NAD(H)-dependent L-alanine dehydrogenase, NAD+ dependent amino acid dehydrogenase, NAD+-dependent alanine dehydrogenase, NAD-dependent alanine dehydrogenase, NAD-linked alanine dehydrogenase, NADH-dependent alanine dehydrogenase, OF4Ald, PlaAlaDH, PvRA, pyruvate reductive aminase, Rv2780, ScALD, SCO1773, SheAlaDH, TB43

ECTree

     1 Oxidoreductases
         1.4 Acting on the CH-NH2 group of donors
             1.4.1 With NAD+ or NADP+ as acceptor
                1.4.1.1 alanine dehydrogenase

Inhibitors

Inhibitors on EC 1.4.1.1 - alanine dehydrogenase

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INHIBITOR
ORGANISM
UNIPROT
COMMENTARY hide
LITERATURE
IMAGE
1-(isonicotinamido)-N2,N4-bis(benzo[d]thiazol-2-yl)azetidine-2,4-dicarboxamide
1-(isonicotinamido)-N2,N4-bis(phenyl)azetidine-2,4-dicarboxamide
lead compound for inhibitor screening, involved in hydrophobic interactions with residues Pro242, Val241, Ala176, Leu130, Ile267, Ala179, Ile199 and Ile174
2,4,6-Trinitrobenzenesulfonic acid
-
inactivation follows pseudo first-order kinetics with a 1:1 stoichiometric ratio between the reagent and the enzyme subunit. Partial protection by each of the substrates, NADH or pyruvate. Complete protection only in presence of the ternary complex enzyme-NADH-pyruvate
2-ethyl-N-phenethyl-5,6,7,8-tetrahydrobenzo[4,5]thieno[2,3-d]pyrimidin-4-amine
the molecule shows activity against nutrient-starved nonreplicating Mycobacterium tuberculosis, resulting in a 2.7 log reduction of bacterial loads at 0.010 mg/ml, and is shown to be more potent than the first-line antitubercular drugs, isoniazid and rifampicin, at the same dose. compound is cytotoxic
-
2-oxobutanoate
-
weak
3-(2-pyridyldithio)propionate
-
inactivation follows pseudo first-order kinetics with a 1:1 stoichiometric ratio between the reagent and the enzyme subunit. Partial protection by each of the substrates, NADH or pyruvate. Complete protection only in presence of the ternary complex enzyme-NADH-pyruvate
3-Bromopyruvate
-
-
3-hydroxypyruvate
-
competitive with respect to pyruvate
4-(furan-2-ylmethylene)-1-phenylpyrazolidine-3,5-dione
the compound exhibited potent antitubercular activity against log-phase cultures of Mycobacterium tuberculosis with a MIC of 0.0245 mM, but is found to be less active than the lead compound 1-(4-chlorophenyl)-4-(4-hydroxy-3-methoxy-5-nitrobenzylidene) pyrazolidine-3,5-dione (CD59). The compound is cytotoxic
-
5'-(p-(fluorosulfonyl)-benzoyl)adenosine
-
inactivation follows pseudo-first-order kinetics, complete inactivation of the enzyme can not be obtained even at high reagent concentration
5,5'-dithiobis(2-nitrobenzoate)
-
-
5-(4-(benzyloxy)benzylidene)-3-(2,5-dimethylphenyl)-2-iminothiazolidin-4-one
compound shows potency, selectivity, and no cytotoxicity up to 50 microM in a mouse macrophage cell line
5-(4-(benzyloxy)benzylidene)-3-(2,6-dimethylphenyl)-2-iminothiazolidin-4-one
compound shows potency, selectivity, and no cytotoxicity up to 50 microM in a mouse macrophage cell line
6-acetyl-2-(4-chlorobenzamido)-4,5,6,7-tetrahydrothieno[2,3-c]pyridine-3-carboxamide
compound shows potency, selectivity, and no cytotoxicity up to 50 microM in a mouse macrophage cell line
AgNO3
-
0.1 mM, 57% inhibition
Ca2+
Alkalihalophilus pseudofirmus
80% inhibition at 10 mM, 71% at 1 mM, 21% at 0.1 mM
CaCl2
Cibacron blue
Cibacron blue F3GA
0.1 mM, 70% inhibition
CoCl2
CuCl2
CuSO4
D-2-aminobutanoate
-
-
D-Ala
D-alanine
AlaDH enzyme activity is completely inhibited by D-alanine in a competitive manner
D-Cys
DTNB
7% inhibition with 0.01 M and 40% inhibition with 0.1 mM
DTT
Alkalihalophilus pseudofirmus
39% inhibition at 10 mM
Fe3+
-
10 mM, 85% inhibition of reductive amination
glycine
glyoxylate
-
weak
HgCl2
Hydroxypyruvate
iodoacetate
KCl
slightly enhances PvRA activity and it moderately promotes ALD activity. In contrast, increasing amounts of KCl gradually inhibits GxRA activity
Ketobutyrate
-
-
L-2-aminobutyrate
-
-
L-alanine
the PvRA activity of ApAlaDH is strongly inhibited by 2 mM L-alanine, 68% inhibition. The GxRA activity is completely inhibited in the presence of L-alanine (100% inhibition at 10 and 20 mM L-alanine)
L-Cys
L-Glutamic acid
-
-
L-Phe
-
reductive amination of pyruvate, 10 mM, 28% inhibition
L-Ser
L-Thr
L-Trp
-
reductive amination of pyruvate, 10 mM, 29% inhibition
Mercurials
-
methyl p-nitrobenzenesulfonate
0.1 mM, complete inhibition
methylglyoxal
-
-
MgCl2
MnCl2
MnSO4
-
10 mM, 50% inhibition
NaCl
NaCl does not inhibit PvRA activity up to 1 M, but inhibition is observed at 2.5 M. The activities of ALD and GxRA are significantly increased in the presence of NaCl at concentrations of 0.1-0.25 M
NADPH
-
inhibition of reductive amination
Ni2+
-
10 mM, 60% inhibition of reductive amination
oxaloacetate
-
-
oxamate
-
-
p-hydroxymercuribenzoate
-
no effect on aminating activity, inhibition of deaminating activity
phosphoenolpyruvate
-
-
propionate
-
-
pyridoxal 5'-phosphate
-
-
pyruvate
tert-butyl 3-carbamoyl-2-(4-chlorobenzamido)-4,5-dihydrothieno[2,3-c]pyridine-6(7H)-carboxylate
compound shows potency, selectivity, and no cytotoxicity up to 50 microM in a mouse macrophage cell line
additional information
-