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molecular biology
development of a synthetic 3-ketosteroid DELTA1-dehydrogenase for the generation of a catabolic pathway enabling cholesterol degradation in human cells
molecular biology
development of a synthetic 3-ketosteroid DELTA1-dehydrogenase for the generation of a catabolic pathway enabling cholesterol degradation in human cells
synthesis
engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry
synthesis
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kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression
synthesis
inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l
synthesis
recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
synthesis
recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
synthesis
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recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
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synthesis
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recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
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synthesis
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kstDF is a promising enzyme in steroid DELTA1-dehydrogenation and steroid reduction that is propitious to construct genetically engineered steroid-transforming recombinants by heterologous overexpression
-
synthesis
-
engineered enzyme mutants, in which KsdDM is inactivated or augmented, are useful for production of phytosterols 4-androstene-3,17-dione or 1,4-androstadiene-3,17-dione, circumventing the difficulty of separating 4-androstene-3,17-dione from 1,4-androstadiene-3,17-dione, a key bottleneck to the microbial transformation of phytosterols in industry
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synthesis
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recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
-
synthesis
-
recombinant enzyme application potential of the former in the synthesis of prednisolone, method evaluation and optimization
-
synthesis
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inactivation of KstD isoforms 1-3 leads to synthesis of about 6.02g/l of 9alpha-hydroxy-4-androstene-3,17-dione from 15 g/l of phytosterols. The product is mixed with 1.55 g/l of 4-androstene-3,17-dione as a major byproduct. Overexpression of the oxygenase component of 3-ketosteroid-9alpha-hydroxylase kshA, results in a yield of 9alpha-hydroxy-4-androstene-3,17-dione of 7.33 g/l
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additional information
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ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis
additional information
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the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant
additional information
3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications
additional information
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3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications
additional information
3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications
additional information
3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications
additional information
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3-oxosteroid DELAT1-dehydrogenases are of particular interest for the etiology of some infectious diseases, for the production of starting materials for the pharmaceutical industry, and for environmental bioremediation applications
additional information
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ksdD-1 and ksdD-2 display respectively high (78%) and low (33%) amino acid sequence identity with the putative ksdD gene of Mycobacterium tuberculosis
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additional information
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the two putative Mycobacterium tuberculosis KsdDs, MT3641 and MT0809, complement the Mycobacterium smegmatis deltaksdD-1 deltaksdD-2 double mutant
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