1.3.99.27: 1-hydroxycarotenoid 3,4-desaturase
This is an abbreviated version!
For detailed information about 1-hydroxycarotenoid 3,4-desaturase, go to the full flat file.
Reaction
Synonyms
1-HO carotenoid 3,4-dehydrogenase, 1-hydroxy monocyclic carotenoid 3,4-dehydrogenase, acyclic carotenoid 3,4-desaturase, CrtD, DDD_2394, hydroxyneurosporene desaturase, P99-3 CrtD
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Substrates Products
Substrates Products on EC 1.3.99.27 - 1-hydroxycarotenoid 3,4-desaturase
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REACTION DIAGRAM
1,1'-dihydroxy-3,4-didehydrolycopene + acceptor
1,1'-dihydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
38% conversion
-
-
?
1,1'-dihydroxyneurosporene + acceptor
1'-hydroxy-1-demethylspheroidene + reduced acceptor
-
39% conversion
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
1-hydroxy-3',4'-didehydrolycopene + acceptor
1-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
28% conversion
-
-
?
1-hydroxy-3,4-didehydrolycopene + acceptor
1-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
-
-
-
?
1-hydroxy-zeta-carotene + acceptor
1-hydroxy-3,4-didehydro-zeta-carotene + reduced acceptor
-
15% conversion
-
-
?
1-hydroxyneurosporene + acceptor
1-demethylspheroidene + reduced acceptor
-
47% conversion. The 3,4-desaturation reaction can only occur with mono-1-hydroxy carotenoids at a Psi-end group or with 1,1'-dihydroxy derivatives carrying a 3'4'-double bond. In addition, 1-hydroxy-zeta-carotene could also be converted by the desaturase. Enzyme kinetic studies show a substrate preference of 1-hydroxyneurosporene over 1-hydroxylycopene
-
-
?
1-hydroxyneurosporene + acceptor
demethylspheroidene + reduced acceptor
-
when O2 is depleted less than 10% of the specific activity is retained. It is rather unlikely that molecular oxygen is the genuine cofactor for hydroxyneurosporene desaturation reactions in Rhodobacter cells when they reach their maximum carotenoid content under anaerobic conditions. Consequently, another, as-yet-unknown electron acceptor may exist in Rhodobacter species that replaces molecular oxygen during anaerobic growth
-
-
?
1-methoxy-1'-hydroxy-3,4-didehydrolycopene + acceptor
1-methoxy-1'-hydroxy-3,4,3',4'-tetradehydrolycopene + reduced acceptor
-
37% conversion
-
-
?
1'-hydroxytorulene + reduced acceptor
-
-
-
-
?
1'-hydroxy-gamma-carotene + acceptor
1'-hydroxytorulene + reduced acceptor
-
15% conversion
-
-
?
1'-hydroxy-gamma-carotene + acceptor
1'-hydroxytorulene + reduced acceptor
-
-
i.e. 3',4'-didehydro-1',2'-dihydroxy-beta,psi-caroten-1'-ol
-
?
1'-hydroxy-gamma-carotene + acceptor
1'-hydroxytorulene + reduced acceptor
-
the enzyme is involved in myxol biosynthesis
-
-
?
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-1,2-dihydrorhodopin + acceptor
1-hydroxy-3,4-didehydro-1,2-dihydrorhodopin + reduced acceptor
-
-
-
-
?
1-hydroxy-3,4-didehydrolycopene + reduced acceptor
-
-
-
-
?
1-hydroxylycopene + acceptor
1-hydroxy-3,4-didehydrolycopene + reduced acceptor
-
33% conversion. The 3,4-desaturation reaction can only occur with mono-1-hydroxy carotenoids at a Psi-end group or with 1,1'-dihydroxy derivatives carrying a 3'4'-double bond. In addition, 1-hydroxy-zeta-carotene could also be converted by the desaturase. Enzyme kinetic studies show a substrate preference of 1-hydroxyneurosporene over 1-hydroxyycopene
-
-
?
1-hydroxylycopene + acceptor
1-hydroxy-3,4-didehydrolycopene + reduced acceptor
-
-
-
-
?
?
-
-
1'-hydroxy-3,4-didehydrolycopene and 1,1'-dihydroxylycopene are poor substrates. 1-Methoxyneurosporene is not converted by purified hydroxyneurosporene desaturase
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-
?
additional information
?
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-
no activity with 1,1'-dihydroxylycopene, 1-methoxyneurosporene, 1'-hydroxydemethylspheroidene, 1,1'-dihydroxy-zeta-carotene, demethylspheroidene and 1'-hydroxyspheroidene
-
-
?