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1.3.98.3: coproporphyrinogen dehydrogenase

This is an abbreviated version!
For detailed information about coproporphyrinogen dehydrogenase, go to the full flat file.

Word Map on EC 1.3.98.3

Reaction

Coproporphyrinogen III
+ 2 S-adenosyl-L-methionine =
protoporphyrinogen IX
+ 2 CO2 + 2 L-methionine + 2 5'-deoxyadenosine

Synonyms

anaerobic coproporphyrinogen III oxidase, At5g63290, AtHEMN1, BtrN, CgoX, Coprogen oxidase, coproporphyrinogen III oxidase, coproporphyrinogen oxidase, coproporphyrinogenase, CPO, EC 1.3.99.22, HemN, HEMN1, HemW, More, oxidase, coproporphyrinogen, oxygen-independent coproporphyrinogen III oxidase, oxygen-independent coproporphyrinogen-III oxidase, oxygen-independent CPO, radical S-adenosyl-L-methionine dehydrogenase, radical SAM enzyme, Sll1876, Sll1917, viperin

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.98 With other, known, physiological acceptors
                1.3.98.3 coproporphyrinogen dehydrogenase

Purification

Purification on EC 1.3.98.3 - coproporphyrinogen dehydrogenase

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
by a single chromatographic step under anaerobic conditions
-
by affinity chromatography
-
His-bind resin column chromatography
-
recombinant GST-tagged enzyme by glutathione affinity chromatography and ultrafiltration
-
recombinant HemN
recombinant HemN from Escherichia coli strain BL21(DE3)
-
recombinant His-tagged full-length enzyme and viperin (214-361) from Escherichia coli strain BL21 inclusion bodies, solubilization by 8 M urea and purification by nickel affinity chromatography and gel filtration
-
recombinant His-tagged HemN from Escherichia coli strain BL21(DE3) membranes by nickel affinity chromatography and gel filtration
-
recombinant terminally Strep-tagged HemNs from Escherichia coli to homogeneity
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recombinant wild-type and mutant HemN
-
Strep-Tactin-Sepharose column chromatography
-