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1.3.98.1: dihydroorotate dehydrogenase (fumarate)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (fumarate), go to the full flat file.

Word Map on EC 1.3.98.1

Reaction

(S)-dihydroorotate
+
fumarate
=
Orotate
 +
succinate

Synonyms

(DHO) dehydrogenase, 4,5-L-dihydroorotate:oxygen oxidoreductase, ACT/DHOD, class 1A DHOD, class 1A DHODH, class 1A dihydroorotate dehydrogenase, DHOD, DHOD1, DHOD2, DHOD3, DHODA, DHOdehase, DHODH, DHODH-1A, dihydroorotate dehydrogenase, dihydroorotate dehydrogenase class 1A, Dihydroorotate oxidase, EC 1.3.3.1, LmDHODH, More, oxidase, dihydroorotate, TcDHOD

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.98 With other, known, physiological acceptors
                1.3.98.1 dihydroorotate dehydrogenase (fumarate)

Cloned

Cloned on EC 1.3.98.1 - dihydroorotate dehydrogenase (fumarate)

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed as His6-tag fusion protein
-
expressed in Escherichia coli
-
expressed in Escherichia coli strain BL21-RP
-
expressed in Escherichia coli strain SØ6645
-
expressed in SO6645 cells
-
expression in Escherichia coli
expression in Escherichia coli strain BL21(DE3) as His-tagged enzyme
-
expression in Plasmodium falciparum
-
expression of His-tagged enzyme
-
genes subcloned int the EcoRI site of pUC18, PCR products cloned in vector pET100/D-TOPO, recombinant DHOD1 and DHOD2 expressed in Escherichia coli BL21-CodonPlus (DE3)-RP
into vector pET-28a and overexpressed in Escherichia coli strain BL21(DE3) as a histidine-tag fusion protein
-
into vector pRSET and expressed in either freshly transformed or glycerol stocked Escherichia coli BL21-CodonPlus (DE3)-RIL
ligated into the pZErO-2 vector and transformation of the Escherichia coli strain TOP 10, inserted into the pET3a expression vector and transformation of Escherichia coli BL21
transformation in Escherichia coli for detection of pyrimidine prototrophic colonies with a resistance of ampicillin
-