1.3.8.4: isovaleryl-CoA dehydrogenase

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For detailed information about isovaleryl-CoA dehydrogenase, go to the full flat file.

Reaction

Synonyms

acyl-CoA dehydrogenase, EC 1.3.99.10, i3VD, iso(3)valeryl-CoA dehydrogenase, isovaleric-CoA dehydrogenase, isovaleroyl-coenzyme A dehydrogenase, isovaleryl-coenzyme A dehydrogenase, IVD, IVDH, LiuA, Pden_3633, SBCAD, short/branched chain acyl-CoA dehydrogenase

ECTree

     1 Oxidoreductases

         1.3 Acting on the CH-CH group of donors

             1.3.8 With a flavin as acceptor

                1.3.8.4 isovaleryl-CoA dehydrogenase

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Results	in table
4	Activating Compound
4063	AA Sequence
3	Application
1	CAS Registry Number
21	Cloned(Commentary)
21	Cofactor
1	Crystallization (Commentary)
88	Disease/ Diagnostics
2	Expression
27	General Information
33	Inhibitors
6	kcat/KM [mM/s]
6	Ki Value [mM]
59	KM Value [mM]
25	Localization
18	Molecular Weight [Da]
57	Natural Substrates/ Products (Substrates)
53	Organism
5	Pathway
10	pH Optimum
49	Protein Variants
15	Purification (Commentary)
1	Reaction
3	Reaction Type
52	Reference
1	Renatured (Commentary)
31	Source Tissue
75	Specific Activity [micromol/min/mg]
1	Storage Stability
136	Substrates and Products (Substrate)
8	Subunits
34	Synonyms
1	Systematic Name
7	Temperature Optimum [°C]
7	Temperature Stability [°C]
17	Turnover Number [1/s]

Purification

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Purification on EC 1.3.8.4 - isovaleryl-CoA dehydrogenase

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PURIFICATION (Commentary)

ORGANISM

UNIPROT

LITERATURE

by loading the supernatant from an induced culture onto an XK16/20 Fast Q fast flow column, overnight dialysis in potassium phosphate buffer, and hydroxyapatite column chromatography

Homo sapiens

P26440

391212

of recombinant enzyme, using sonication, ammonium sulfate fractionation, column chromatography on DEAE-Sepharose and hydroxyapatite

Caenorhabditis elegans

-

391218

partially from tuber, recombinant from Escherichia coli to over 90% purity

Solanum tuberosum

Q9FS87

656288

recombinant from Escherichia coli to over 90% purity

Homo sapiens

-

656288

recombinant LiuA from Escherichia coli

Pseudomonas aeruginosa

-

692393

recombinant Strep-tagged wild-type and mutant enzymes from Escherichia coli strain BL21(DE3) by affinity chromatography

Paracoccus denitrificans

A1B856

763557

using ammonium sulfate fractionation followed by DEAE-Sephadex A-50, hydroxyapatite, Matrex Gel Blue A, agarose-hexane-CoA, and Bio-Gel A-0.5 column chromatography

Homo sapiens

-

391201

using auxin affinity chromatography matrix

Pisum sativum

-

391215

using bacterial lysates through centrifugation, filtration through an Acrodisc filter, chromatography on a XK16/20 DEAE fast-flow column and hydroxyapatite resin column

Homo sapiens

-

391220

using chromatography on Resource Q anion exchange column, BioRad CHT2 hydroxyapatite column and phenyl ether hydrophobic interaction column

Solanum tuberosum

-

391216

using DEAE-Sepharose followed by hydroxyapatite column chromatography

Homo sapiens

-

391211

using ion exchange column chromatography and isoelectric focusing

Rattus norvegicus

-

391205

using sonication, ammonium sulfate treatment and column chromatography on DEAE-Sephadex A-50, hydroxylapatite, Matrex Gel Blue A and BioGel A-0.5 m

Rattus norvegicus

-

391200, 391202

using sonication, ammonium sulfate treatment, column chromatography on DEAE-Sephadex A-50, hydroxylapatite and isoelectrofocusing

Rattus norvegicus

-

391203