1.3.8.12: (2S)-methylsuccinyl-CoA dehydrogenase
This is an abbreviated version!
For detailed information about (2S)-methylsuccinyl-CoA dehydrogenase, go to the full flat file.
Reaction
Synonyms
MCD, methylsuccinyl-CoA dehydrogenase, PdMCD
ECTree
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Purification
Purification on EC 1.3.8.12 - (2S)-methylsuccinyl-CoA dehydrogenase
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HisTrap column chromatography and Superdex 200 gel filtration
recombinant His-tagged wild-tpye and mutant enzymes from Escherichia coli strain Rosetta pLys (DE3) by nickel affinity chromatography of cell-free supernatant, desalting gel filtration, tag cleavage by thrombin, gel filtration, and ultrafiltration
recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain Rosetta (DE3) pLysS by nickel affinity chromatography and ultrafiltration