1.3.5.5: 15-cis-phytoene desaturase
This is an abbreviated version!
For detailed information about 15-cis-phytoene desaturase, go to the full flat file.
Word Map on EC 1.3.5.5
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1.3.5.5
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carotenoid
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herbicide
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zeta-carotene
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agriculture
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bleaching
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norflurazon
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synthesis
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phytofluene
- 1.3.5.5
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carotenoid
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herbicide
- zeta-carotene
- agriculture
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bleaching
- norflurazon
- synthesis
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phytofluene
Reaction
Synonyms
BoPDS, CitPDS1, CrtP, OsPDS, PDS, PDS1, PDS3, phytoene desaturase, plant-type phytoene desaturase
ECTree
1.3.5.5 15-cis-phytoene desaturaseAdvanced search results
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results (Inhibitors
Inhibitors on EC 1.3.5.5 - 15-cis-phytoene desaturase
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INHIBITOR 
ORGANISM
UNIPROT
COMMENTARY 
LITERATURE
IMAGE
(2R,5S)-5-methyl-2-(3-trifluoromethylbenzyl)-3-oxomorpholine
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less active inhibitor compared to (2R,5S)-5-methyl-2-(3-trifluoromethylbenzyl)-3-ketomorpholine; very low inhibition
O-[1-ethyl-2-(3-trifluoromethylphenoxy)]ethyl-N-(2-chlorobenzyl)carbamate
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O-[1-ethyl-2-(3-trifluoromethylphenoxy)]ethyl-N-(2-phenylethyl)carbamate
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O-[1-methyl-2-(3-trifluoromethylphenoxy)]ethyl-N-benzylcarbamate
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additional information
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with respect to a structure-activity relationship the oxygen atom of the phenoxy group and a carbamate structure in O-(1-ethyl-2-phenoxy)ethyl-N-aralkylcarbamates studied are found to be essential for strong PDS inhibitors. phytoene desaturase inhibitor
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fluridone
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arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
norflurazon
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arginine304 present in the wild-type Hydrilla verticillata phytoene desaturase is substituted with all 19 other natural amino acids. In vitro the threonine (Thr), cysteine (Cys), alanine (Ala) and glutamine (Gln) mutations impart the highest resistance to fluridone. The three natural mutations [Cys, serine (Ser), histidine (His)] and the wild-type phytoene desaturase protein are tested in vitro against seven inhibitors of phytoene desaturase representing several classes of herbicides. These mutations confer cross-resistance to norflurazon and overall negative cross-resistance to beflubutamid, picolinafen and diflufenican. The Thr304 Hydrilla pds mutant is an excellent marker for the selection of transgenic plants. Seedlings harbouring Thr304 pds have a maximum resistance to sensitivity (R/S) ratio of 57 and 14 times higher than that of the wild-type for treatments with norflurazon and fluridone, respectively. These plants exhibit normal growth and development, even after long-term exposure to herbicide. As Thr304 pds is of plant origin, it could become more acceptable than other selectable markers for use in genetically modified food
norflurazon
arrests the reaction by stabilizing the intermediary FADred. An excess of the quinone acceptor relieves this blockage
norflurazon
NFZ, competitive inhibition, inhibition kinetics with enzyme mutant R300S
norflurazon
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0.0001 mM, Vmax values are very little influenced by norflurazon, which reveals a competition between NADP+ or plastoquinone and norflurazon for the cofactor binding site of phytoene desaturase
