1.3.5.2: dihydroorotate dehydrogenase (quinone)
This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (quinone), go to the full flat file.
Word Map on EC 1.3.5.2
-
1.3.5.2
-
pyrimidine
-
falciparum
-
plasmodium
-
leflunomide
-
brequinar
-
uridine
-
malaria
-
arthritis
-
antimalarial
-
rheumatoid
-
dihydroorotase
-
salvage
-
teriflunomide
-
dhods
-
autoimmune
-
orotidine
-
ump
-
atovaquone
-
triazolopyrimidine
-
flavoenzyme
-
medicine
-
phosphoribosyltransferase
-
antirheumatic
-
coq
-
drug development
-
decylubiquinone
-
dmards
-
synthesis
-
species-selective
-
pyre
-
pharmacology
-
isoxazole
-
transcarbamoylase
-
5-fluoroorotate
- 1.3.5.2
- pyrimidine
- falciparum
- plasmodium
- leflunomide
- brequinar
- uridine
- malaria
- arthritis
-
antimalarial
-
rheumatoid
- dihydroorotase
-
salvage
- teriflunomide
-
dhods
- autoimmune
- orotidine
- ump
- atovaquone
- triazolopyrimidine
-
flavoenzyme
- medicine
-
phosphoribosyltransferase
-
antirheumatic
- coq
- drug development
- decylubiquinone
-
dmards
- synthesis
-
species-selective
-
pyre
- pharmacology
-
isoxazole
-
transcarbamoylase
- 5-fluoroorotate
Reaction
Synonyms
class 2 dihydroorotate dehydrogenases, DHO-DH, DHOD, DHODase, DHODH, dihydroorotate dehydrogenase, EC 1.3.99.11, ETH_00004975, hDHODH, HsDHODH, L-5,6-dihydroorotate:ubiquinone exidoreductase, PfDHODH
ECTree
1.3.5.2 dihydroorotate dehydrogenase (quinone)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.3.5.2 - dihydroorotate dehydrogenase (quinone)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
orotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinol
-
-
-
-
?
(S)-dihydroorotate + 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
orotate + reduced 2,3-dimethoxy-5-methyl-6-(3-methyl-2-butenyl)-1,4-benzoquinone
Q02172
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
orotate + reduced 2,6-dihydrophenolindophenol
(S)-dihydroorotate + 5,8-dihydroxy-1,4-naphthoquinone
orotate + 5,8-dihydroxy-1,4-naphthoquinol
78% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q2
orotate + reduced coenzyme Q2
123% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + oxidized 2,6-dichloroindophenol
orotate + reduced 2,6-dichloroindophenol
-
-
-
-
?
dihydroorotate + 2,3-dimethoxy-5-methyl-1,4-benzoquinone
orotate + reduced 2,3-dimethoxy-5-methyl-1,4-benzoquinone
-
-
-
?
dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + reduced 2,5-dimethyl-p-benzoquinone
95% the rate of decylubiquinone, wild-type, 75% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
dihydroorotate + 2,5-dimethylbenzoquinone
orotate + reduced 2,5-dimethylbenzoquinone
-
84% of the rate with decylubiquinone
-
-
?
dihydroorotate + 2,6-dichloroindophenol
orotate + reduced 2,6-dichloroindophenol
-
-
-
?
dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
dihydroorotate + coenzyme Q
orotate + reduced coenzyme Q
coenzyme OD possesses a decyl tail
-
-
?
dihydroorotate + coenzyme Q1
orotate + reduced coenzyme Q1
coenzyme Q1 possesses a prenyl tail
-
-
?
dihydroorotate + coenzyme QD
orotate + reduced coenzyme QD
coenzyme QD possesses a decyl tail
-
-
?
dihydroorotate + CoQ0
orotate + reduced CoQo
102% the rate of decylubiquinone, wild-type, 67% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
dihydroorotate + potassium hexacyanoferrate(III)
orotate + reduced potassium hexacyanoferrate(III)
-
-
-
?
dihydroorotate + ubiquinone 30
orotate + reduced ubiquinone 30
-
-
-
?
dihydroorotate + ubiquinone 50
orotate + reduced ubiquinone 50
-
-
-
?
dihydroorotate + ubiquinone-0
orotate + reduced ubiquinone-0
-
80% of the rate with decylubiquinone
-
-
?
dihydroorotate + ubiquinone-10
orotate + reduced ubiquinone-10
-
59% of the rate with decylubiquinone
-
-
?
dihydroorotate + ubiquinone-50
orotate + reduced ubiquinone-50
83% the rate of decylubiquinone, wild-type, 76% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
L-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
L-dihydroorotate + 2,6-dihydrophenolindophenol
orotate + reduced 2,6-dihydrophenolindophenol
-
-
-
-
r
L-dihydroorotate + ubiquinone-0
orotate + reduced ubiquinone-0
-
-
-
?
L-dihydroorotate + ubiquinone-4
orotate + reduced ubiquinone-4
-
-
-
?
L-dihydroorotate + vitamin K3
orotate + reduced vitamin K3
-
-
-
?
methyl-dihydroorotate + acceptor
methyl-orotate + reduced acceptor
-
-
-
-
?
methyl-S-dihydroorotate + 2,6-dichlorophenolindophenol
methylorotate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
r
tert-butyl-dihydroorotate + acceptor
tert-butyl-orotate + reduced acceptor
-
-
-
-
?
(S)-dihydroorotate + 1,4-naphthoquinone
orotate + 1,4-naphthoquinol
50% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + 1,4-naphthoquinone
orotate + 1,4-naphthoquinol
78% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + 1,4-naphthoquinone
orotate + 1,4-naphthoquinol
-
-
-
-
?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + 2,5-dimethyl-p-benzoquinol
48% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + 2,5-dimethyl-p-benzoquinol
67% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + 2,5-dimethyl-p-benzoquinone
orotate + 2,5-dimethyl-p-benzoquinol
-
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
Q02172
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
orotate + reduced 2,6-dihydrophenolindophenol
-
-
-
-
r
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
orotate + reduced 2,6-dihydrophenolindophenol
-
-
-
?
(S)-dihydroorotate + 2,6-dihydrophenolindophenol
orotate + reduced 2,6-dihydrophenolindophenol
-
-
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fumarate and menadione as electron acceptors
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
reduction of a number of substrates: fumatate, coenzyme ubiquinone-0 and menadione
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
uses ubiquinone as electron acceptor
-
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
with ubiquinone-0, 2,6-dichlorophenolindophenol, menadione, decylubiquinone, fumarate and O2 as electron acceptor
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in synthesis of pyrimidine nucleotides
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in synthesis of pyrimidine nucleotides
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in synthesis of pyrimidine nucleotides
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
ubiquinone-6 tested as electron acceptor
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
with ubiquinone-0, 2,6-dichlorophenolindophenol, menadione, decylubiquinone, fumarate and O2 as electron acceptor
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in synthesis of pyrimidine nucleotides
-
?
(S)-dihydroorotate + coenzyme Q0
orotate + reduced coenzyme Q0
56% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q0
orotate + reduced coenzyme Q0
96% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q1
orotate + reduced coenzyme Q1
111% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q1
orotate + reduced coenzyme Q1
124% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q10
orotate + reduced coenzyme Q10
30% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q10
orotate + reduced coenzyme Q10
19% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q6
orotate + reduced coenzyme Q6
144% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q6
orotate + reduced coenzyme Q6
102% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q9
orotate + reduced coenzyme Q9
66% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Q9
orotate + reduced coenzyme Q9
111% of the activity with coenzyme Qd
-
-
?
(S)-dihydroorotate + coenzyme Qd
orotate + reduced coenzyme Qd
-
-
-
?
(S)-dihydroorotate + coenzyme Qd
orotate + reduced coenzyme Qd
-
-
-
?
(S)-dihydroorotate + decylubiquinone
orotate + decylubiquinol
-
-
-
-
r
(S)-dihydroorotate + decylubiquinone
orotate + decylubiquinol
-
-
-
-
?
(S)-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
?
(S)-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
Q02172
-
-
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
reaction studied with menadione, O2 and ferricyanide
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
decylubiquinone as electron acceptor
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth enzyme in pyrimidine synthesis
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth enzyme in pyrimidine synthesis
-
ir
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
six-step biosynthesis of pyrimidine uridine monophosphate
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth step in synthesis of uridine 5'-monophosphate
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth enzyme in pyrimidine synthesis
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth enzyme in pyrimidine synthesis
-
ir
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
ubiquinone-6, ubiquinone-7, ubiquinone-9 and ubiquinone-10 maximize enzyme activity
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth step in synthesis of uridine 5'-monophosphate
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
fourth enzyme in pyrimidine synthesis
-
?
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
fourth enzyme in pyrimidine synthesis
-
ir
(S)-dihydroorotate + ubiquinone
orotate + ubiquinol
-
six-step biosynthesis of pyrimidine uridine monophosphate
-
?
dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
33% of the rate with decylubiquinone
-
-
?
dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: coenzyme Q6
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: menadione
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: fumarate, coenzyme Q10
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
mechanism and pH-dependence of reaction
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: 2,6-dichlorophenolindophenol
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: menaquinone
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: ubiquinone
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
reaction intermediates, mechanism, pH-dependence of reaction
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
physiological electron acceptor: ubiquinone (aerobic conditions), menaquinone (anaerobic conditions)
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
-
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor (low activity): 1,4-naphthoquinone, 5,8-hydroxy-naphthoquinone, juglon, plumbagin, polyporic acid
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: ubiquinone
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: 2,6-dichlorophenolindophenol
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: quinones
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
not acceptor: oxygen
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
cytochrome b, cytochrome c, pyridine nucleotides (NAD+, NADP+) cannot serve as electron acceptors
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: quinones
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: 2,6-dichlorophenolindophenol
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptors: e.g. phenazine methosulfate
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor (low activity): 1,4-naphthoquinone, 5,8-hydroxy-naphthoquinone, juglon, plumbagin, polyporic acid
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: ubiquinone
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: oxygen, slowly
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in pyrimidine biosynthesis
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: ubiquinone
-
-
?
dihydroorotate + coenzyme Q0
orotate + reduced coenzyme Q0
-
-
-
?
dihydroorotate + coenzyme Q0
orotate + reduced coenzyme Q0
-
-
-
?
dihydroorotate + decylplastoquinone
orotate + reduced decylplastoquinone
95% the rate of decylubiquinone, wild-type, 104% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
dihydroorotate + decylplastoquinone
orotate + reduced decylplastoquinone
-
78% of the rate with decylubiquinone
-
-
?
dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
?
dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
?
dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
-
?
dihydroorotate + menadione
orotate + reduced menadione
82% the rate of decylubiquinone, wild-type, 78% the rate of decylubiquinone, DELTA1-75 mutant
-
-
?
dihydroorotate + menadione
orotate + reduced menadione
-
39% of the rate with decylubiquinone
-
-
?
L-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
-
?
L-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
?
L-dihydroorotate + decylubiquinone
orotate + reduced decylubiquinone
-
-
-
-
?
L-dihydroorotate + FMN
orotate + FMNH2
-
DHODH catalyzes the FMN-dependent oxidation of dihydroorotate to produce orotic acid. Two separate half reactions are required to complete the catalytic cycle: 1. oxidation of dihydroorotate driven by the reduction of FMN and, 2. reoxidation of FMNH2 to regenerate the active enzyme
-
-
?
L-dihydroorotate + FMN
orotate + FMNH2
DHODH catalyzes the FMN-dependent oxidation of dihydroorotate to produce orotic acid. Two separate half reactions are required to complete the catalytic cycle: 1. oxidation of dihydroorotate driven by the reduction of FMN and, 2. reoxidation of FMNH2 to regenerate the active enzyme
-
-
?
L-dihydroorotate + ubiquinone-6
orotate + reduced ubiquinone-6
-
-
-
-
?
L-dihydroorotate + ubiquinone-6
orotate + reduced ubiquinone-6
-
-
-
?
additional information
?
-
-
not substrate: 1-methyl-(S)-dihydroorotate, 3-methyl-dihydroorotate, 1,3-dimethyldihydroorotate
-
-
?
additional information
?
-
fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH
-
-
?
additional information
?
-
-
fumarate and NAD are inadequate electron acceptors for full-length DHODH and N-terminally truncated DHODH
-
-
?
additional information
?
-
computational method to investigate potential proton relay pathways in the active site of the enzyme
-
-
?
additional information
?
-
-
computational method to investigate potential proton relay pathways in the active site of the enzyme
-
-
?
additional information
?
-
reduction of decylubiquinone (DUQ) is stoichiometrically equivalent to reduction of 2,6-ichloroindophenol (DCIP) resulting in the decrease in absorbance at 610 nm, which is measured
-
-
-
additional information
?
-
-
reduction of decylubiquinone (DUQ) is stoichiometrically equivalent to reduction of 2,6-ichloroindophenol (DCIP) resulting in the decrease in absorbance at 610 nm, which is measured
-
-
-
additional information
?
-
-
enzyme has methylviologen-fumarate reductase activity
-
-
?
