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1.3.5.2: dihydroorotate dehydrogenase (quinone)

This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (quinone), go to the full flat file.

Word Map on EC 1.3.5.2

Reaction

(S)-dihydroorotate
+
a quinone
=
Orotate
 +
a quinol

Synonyms

class 2 dihydroorotate dehydrogenases, DHO-DH, DHOD, DHODase, DHODH, dihydroorotate dehydrogenase, EC 1.3.99.11, ETH_00004975, hDHODH, HsDHODH, L-5,6-dihydroorotate:ubiquinone exidoreductase, PfDHODH

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.5 With a quinone or related compound as acceptor
                1.3.5.2 dihydroorotate dehydrogenase (quinone)

Storage Stability

Storage Stability on EC 1.3.5.2 - dihydroorotate dehydrogenase (quinone)

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STORAGE STABILITY
ORGANISM
UNIPROT
LITERATURE
-18°C, stable
-
-20°C, 10 mg/ml, 50 mM sodium phosphate, pH 7.0, 0.1 mM EDTA, 50% glycerol
-
-20°C, 50% glycerol, 1 mM DTT, 0.01 mM FMN, no loss of activity during time of studies
-
-20°C, several days
-
-70°C, flash frozen
-
-80°C
-
-80°C, addition of S-dihydroorotate to stabilize the enzyme activity
-
-80°C, slight activity loss after freezing and storage
4°C, 12 days, 10 mM dihydroorotate
-
4°C, extremely labile, 90% decrease of activity over night
-
4°C, pH 9.0, 50 mM Tris/HCl buffer, 0.1% Triton X-100, 3-4 weeks, t1/2: 2.5 months
-
great inactivation by repeated thawing and freezing