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1.3.3.4: protoporphyrinogen oxidase

This is an abbreviated version!
For detailed information about protoporphyrinogen oxidase, go to the full flat file.

Word Map on EC 1.3.3.4

Reaction

protoporphyrinogen IX
+ 3 O2 =
protoporphyrin IX
+ 3 H2O2

Synonyms

HemG, HemG-type PPO, HemG-type protoporphyrinogen IX oxidase, hemY, hPPO, H_N10, H_N40, H_N90, LMJF_06_1280, mtPPO, MxPPOX, MxProtox, PPO, PPO1, ppo1-1, PPO2, PPOX, PPOX I, PPX1, PPX2, protein YfeX, protogen oxidase, protoporphyrinogen IX oxidase, protoporphyrinogen IX oxidase 1, protoporphyrinogen oxidase, protoporphyrinogen oxidase IX, protoporphyrinogenase, protox, Protox enzyme, R-PPO, Rs-slr1790 protein, S-PPO, Salk_143057, YfeX

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.4 protoporphyrinogen oxidase

Crystallization

Crystallization on EC 1.3.3.4 - protoporphyrinogen oxidase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
purified native and SeMet-labeled enzyme in complex with inhibitor acifluorfen, sitting-drop, vapor-diffusion method, 10 mg/ml wild-type bsPPO protein in 50 mM TrisHCl, pH 7.5, 200 mM sodium chloride, 5 mM DTT, 1 mM EDTA, and acifluorfen in a 1:5 ratio, equilibrating against a reservoir solution of 2.4 M ammonium phosphate dibasic, 0.1 M Tris-HCl, pH 7.5, 1 week, 20°C, X-ray diffraction structure determination and analysis at 2.8-3.5 A resolution
crystal structure analysis
-
by the hanging drop vapor diffusion method at 16°C in the dark
-
purified selenomethionine-labeled, recombinant mitochondrial isozyme PPO2 free or in complex with inhibitor 4-bromo-3-(5'-carboxy-4'-chloro-2'-fluoro-phenyl)-1-methyl-5-trifluoromethyl-pyrazol and with FAD, protein solution contains protein, 5 mM Tris-HCl, pH 8.0, 0.1% Triton X-100, 50 mM sodium chloride, hanging drop vapour diffusion method, 18°C, precipitant solution contains 10% PEG 1000, 0.1 M sodium citrate-HCl, pH 4.0, and 0.2 M sodium chloride, 2 days, selenium single anomalous X-ray diffraction structure determination and analysis at 2.9 A resolution, molecular modelling