1.3.3.3: coproporphyrinogen oxidase
This is an abbreviated version!
For detailed information about coproporphyrinogen oxidase, go to the full flat file.
Word Map on EC 1.3.3.3
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1.3.3.3
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heme
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protoporphyrinogen
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coproporphyria
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protoporphyrin
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porphyria
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ferrochelatase
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uroporphyrinogen
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5-aminolevulinic
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porphobilinogen
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tetrapyrrole
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porphyrinogens
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medicine
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oxygen-independent
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coproporphyrinuria
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mg-protoporphyrin
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ala-pdt
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delta-aminolaevulinic
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uroporphyrin
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cutanea
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analysis
- 1.3.3.3
- heme
- protoporphyrinogen
- coproporphyria
- protoporphyrin
- porphyria
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ferrochelatase
- uroporphyrinogen
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5-aminolevulinic
- porphobilinogen
- tetrapyrrole
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porphyrinogens
- medicine
-
oxygen-independent
-
coproporphyrinuria
- mg-protoporphyrin
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ala-pdt
-
delta-aminolaevulinic
-
uroporphyrin
-
cutanea
- analysis
Reaction
Synonyms
copro'gen oxidase, Coprogen oxidase, coproporphyinogen oxidase, coproporphyrinogen III oxidase, coproporphyrinogen oxidase, coproporphyrinogen-III oxidase, coproporphyrinogenase, COX, CPgen oxidase, CPGox, CPO, CPO III oxidase, CPOX, CPOX4, CPX, CPX1, CPX2, HEM13, Hem13p, HemF, HEMN1, KlHEM13, LIN2, LMM2, O2-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen-III oxidase, Sll1185
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Purification
Purification on EC 1.3.3.3 - coproporphyrinogen oxidase
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ammonium sulfate precipitation, DEAE-Sepharose column chromatography, and Ultrogel Ac44 gel filtration
by Ni2+ affinity chromatography
partial
recombinant enzyme from Escherichia coli, 3-step procedure, 119fold to homogeneity
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recombinant HA-tagged wild-type enzyme and His-tagged mutant enzyme from Escherichia coli by nickel and heparin affinity chromatography, respectively
recombinant hemF oxygen-dependent enzyme form to homogeneity by affinity chromatography, the tag protein is cleaved off by thrombin, recombinant wild-type and mutant enzymes
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recombinant His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
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recombinant His-tagged enzyme from Escherichia coli, cleavage of the tag
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recombinant terminally Strep-tagged HemF from Escherichia coli to homogeneity
separation of coproporphyrinogen oxidase, EC 1.3.3.3, from uroporphyrinogen decarboxylase, EC 4.1.1.37, by coproporphyrinogen-III-resin affinity chromatography, elution in a salt gradient
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separation of uroporphyrinogen decarboxylase, EC 4.1.1.37, from coproporphyrinogen oxidase using porphyrin-affinity chromatography
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to homogeneity, chromatography steps
to homogeneity, recombinant enzyme