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1.3.3.3: coproporphyrinogen oxidase

This is an abbreviated version!
For detailed information about coproporphyrinogen oxidase, go to the full flat file.

Word Map on EC 1.3.3.3

Reaction

Coproporphyrinogen III
+
O2
+ 2 H+ =
protoporphyrinogen-IX
+ 2 CO2 + 2 H2O

Synonyms

copro'gen oxidase, Coprogen oxidase, coproporphyinogen oxidase, coproporphyrinogen III oxidase, coproporphyrinogen oxidase, coproporphyrinogen-III oxidase, coproporphyrinogenase, COX, CPgen oxidase, CPGox, CPO, CPO III oxidase, CPOX, CPOX4, CPX, CPX1, CPX2, HEM13, Hem13p, HemF, HEMN1, KlHEM13, LIN2, LMM2, O2-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen III oxidase, oxygen-dependent coproporphyrinogen-III oxidase, Sll1185

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.3 coproporphyrinogen oxidase

Cloned

Cloned on EC 1.3.3.3 - coproporphyrinogen oxidase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
6x-his-tagged enzyme expressed in Escherichia coli
-
cpx1 cloned into LambdaZAP
DNA and amino acid sequence determination and analysis, eighteen functional C/EBP binding motifs in the mCPO promoter are found, all are predicted to bind C/EBPs with moderate or higher affinity
DNA and amino acid sequence determination and analysis, the murine CPO gene upstream region contains many competent C/EBP binding sites
DNA sequence determination and analysis of wild-type and mutant genes
-
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)RIL cells
expressed in Escherichia coli strain BL21(DE3)RIL
expressed in Escherichia coli with the vector pET21d-CO
-
expression in Cos-1 cells
-
expression in Escherichia coli
expression in Escherichia coli BL21(DE3)RIL
-
expression of the His-tagged enzyme in Escherichia coli strain BL21(DE3)
-
functional expression in Escherichia coli
-
gene AtCPO-1, chromosome I, DNA and amino acid sequence determination and analysis, complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, expression as GFP-fusion protein in transgenic Arabidopsis thaliana plants via Agrobacterium tumefaciens tranbsformation
gene AtCPO-2, DNA sequence determination and analysis, no complementation of an enzyme-deletion Saccharomyces cerevisiae hem13 mutant, possibly a pseudogene
gene CPO, located on chromosome 3q11.2, DNA and amino acid sequence determination and analysis, genotyping of an Italian population
-
gene hemF, recombinant overexpression in Aspergillus niger, subcloning in Escherichia coli strain DH5alpha, complementation of a hemF deletion strain
gene LIN2, construction of transgenic plants expressing the mutant LIN2 gene using Agrobacterium tumefaciens strain GV3101 transfection
gene sll1185, overexpression of N-terminally Strep-tagged HemF in Escherichia coli
in-vitro transcription and translation of the enzyme
-
into pAB24, expression in Saccharomyces cerevisiae BY4743(hem13::kanMX4), wild-type Saccharomyces cerevisiae and Rox1p and Mga2p mutants transformed with an episomic plasmid derived from YEP365, in which KlHEM13 promoter is fused in frame to lacZ
into the MscI site of pUI1087, generating pKS7, moving of the hemF sequences from pKS7 into pBBR1MCS2, creating pKS9, BamHI DNA fragment deleted from pKS9, omega Sp-St resistance cassette inserted at the remaining unique BamHI site, generating pKS11, from pKS11 3135-bp SmaI fragment containing delta hemF::omegaSpr-Str moved into pSUP202, replacing the vector sequences contained on a 1,348-bp ScaI fragment and creating pKS13, hemF::lacZ transcription reporter plasmid pJZ84 constructed by first inserting a 717-bp SmaI-NcoI DNA fragment from pKS9 into pUI1087, using the flanking PstI-XbaI vector restriction sites, the hemFequences are positioned and correctly oriented in front of a promoterless lacZ gene in plasmid vector pCF1010
-
into vector PCRII-TOPO, transferred into vector pcDNA3.1(-), and transfection of LNCaP cells and Cos-7 cells
-
into vector pET21d
into vector PTZ57R/T
-
mature form of wild-type CPOX cloned into pET-22, wild-type and mutant protein expressed in Escherichia coli BL21(DE3)plysS
mutant enzyme cloned into pGEX-2T and expressed in Escherichia coli
-
northern hybridization analysis
-
over 200fold overexpression in Escherichia coli strain BL21(DE3), reduction of the toxicity of product protoporphyrin-IX for the bacterial cells by decrease of growth temperature to 20°C
-
overexpression in Escherichia coli
-
overexpression of hemF oxygen-dependent enzyme form as N-terminally fusion protein with calmodulin-binding-peptide, overexpression of wild-type and mutant enzymes in strain BL21(DE3)
-
recombinant coexpression of HA-tagged wild-type enzyme from healthy donors and of His-tagged mutant enzyme from HCP patients in Escherichia coli strain BL21, the His-enzyme forms a heterodimer in association with the HA-tagged enzyme, The monomeric form of mutated CPOX does not show any activity and homodimeric enzymes derived from HCP mutant show low activity (about 20% of the control). The chimeric heterodimers with wild-type and mutated subunits from HCP patients show low protoporphyrinogen producing activity. The active site of the enzyme involved in the second step of decarboxylation is encoded in exon 6
recombinant expression of His-tagged enzyme in Escherichia coli
-