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1.3.3.11: pyrroloquinoline-quinone synthase

This is an abbreviated version!
For detailed information about pyrroloquinoline-quinone synthase, go to the full flat file.

Word Map on EC 1.3.3.11

Reaction

6-(2-amino-2-carboxyethyl)-7,8-dioxo-1,2,3,4,7,8-hexahydroquinoline-2,4-dicarboxylate
+ 3 O2 =
4,5-dioxo-4,5-dihydro-1H-pyrrolo[2,3-f]quinoline-2,7,9-tricarboxylate
 + 2 H2O2 + 2 H2O

Synonyms

PqqC, PqqC/D, pyrroloquinoline quinone synthase C, pyrroloquinoline-quinone synthase, pyrroloquinoline/quinone synthesis protein C, quinoprotein dehydrogenase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.3 With oxygen as acceptor
                1.3.3.11 pyrroloquinoline-quinone synthase

Cloned

Cloned on EC 1.3.3.11 - pyrroloquinoline-quinone synthase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3)
expressed in Escherichia coli BL21(DE3) cells
-
expressed in Escherichia coli BL21(DE3)pLysS, complements the mineral phosphate solubilization phenotype
-
expressed in Escherichia coli DH alpha, complementation studies with PqqC deficient Methylobacterium extorquens and Methylobacterium organophilum
expressed in Escherichia coli DHalpha
-
gene pqqC, enzymes that biosynthesize PQQ are encoded by the pqqABCDE gene cluster and the tldD gene. The tufB promoter is ligated withpqqA, pqqB, pqqC, pqqD, pqqE and tldD, and the pqqA promoter is ligated with pqqB, pqqC, pqqD and pqqE, respectively, through fusionPCR, subcloning in Escherichia coli. Overexpression Gluconobacter oxydans strain WSH-003 of pqqA, pqqB, pqqC, pqqD and pqqE with the pqqA promoter enhances the extracellular PQQ concentration by 38.5%, 68.4%, 19.9%, 30.3% and 8.2%, respectively, whereas production is enhanced by 59.4%, 85.7%, 30.9%, 42.2%, and 19.3%, respectively, using the tufB promoter. Metabolic effects, overview
gene pqqC, expression of wild-type and mutant enzymes as His-tagged enzyme in Escherichia coli strain BL21(DE3)
gene pqqC, from a Pseudomonas strain collection and from a natural wheat rhizosphere population, DNA and amino acid sequence determination and analysis, phylogenetic analysis
-
gene pqqC, genetic organization of the pqqC gene region on the chromosome of Pseudomonas kilonensis JX22, sequence comparisons and phylogenetic analysis