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1.3.1.75: 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

This is an abbreviated version!
For detailed information about 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH), go to the full flat file.

Word Map on EC 1.3.1.75

Reaction

protochlorophyllide a
+
NADP+
=
3,8-divinyl protochlorophyllide a
+
NADPH
+
H+

Synonyms

3,8-divinyl (proto)chlorophyll(ide) a 8-vinyl reductase, 3,8-divinyl chlorophyllide reductase, 4VCR, 8-vinyl reductase, 8V reductase, 8VR, AM1_2394, AtDVR, AtN-DVR, BciA, BciA-type 8VR, bciB, C8 vinyl reductase, C8-vinyl reductase, C8V reductase, COR, CsDVR, CTBciA, ct_1063, cvrA, divinyl chlorophyll(ide) a reductase, divinyl chlorophyllide a 8-vinyl-reductase, divinyl chlorophyllide a 8-vinyl-reductase, chloroplastic, divinyl reductase, divinyl-chlorophyllide a reductase, DVR, N-DVR, NADPH-dependent 8VR, NADPH-dependent divinyl reductase, NADPH-dependent DVR, NADPH-dependent reductase BciA, nmrA, nucleoside-diphosphate-sugar epimerase, Os03g22780, OsDVR, PCB2, PhaeN-DVR, reductase, 4-vinylchlorophyllide a, RSBciA, RSP_3070, synw0963, ZmDVR, [3,8-divinyl]-protochlorophyllide a 8-vinyl reductase, [3,8-DV]-Chlide a 8-vinyl reductase, [4-vinyl]chlorophyllide a reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.75 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

Purification

Purification on EC 1.3.1.75 - 3,8-divinyl protochlorophyllide a 8-vinyl-reductase (NADPH)

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PURIFICATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
partial
recombinant enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography
recombinant N-terminally Strep II-tagged enzyme from Escherichia coli strain Rosetta 2 by affinity chromatography and gel filtration
Strep-Tactin Sepharose column chromatography and Sephacryl S-200 gel filtration