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1.3.1.33: protochlorophyllide reductase

This is an abbreviated version!
For detailed information about protochlorophyllide reductase, go to the full flat file.

Word Map on EC 1.3.1.33

Reaction

chlorophyllide a
+
NADP+
=
protochlorophyllide
 +
NADPH
 +
H+

Synonyms

3PCR, B9Z40_03660, bchB, bchL, bchN, dark active protochlorophyllide oxidoreductase, dark operative protochlorophyllide oxidoreductase, dark-operative protochlorophyllide oxidoreductase, DPOR, EH32_03160, faded green leaf, Fgl, GEMMAAP_15250, LHPP, light-activated enzyme protochlorophyllide oxidoreductase, light-dependent NADPH-protochlorophyllide oxidoreductase, light-dependent NADPH: protochlorophyllide oxidoreductase, light-dependent NADPH:Pchlide oxidoreductase, light-dependent NADPH:protochlorophyllide oxidoreductase, light-dependent Pchlide oxidoreductase, Light-dependent protochlorophyllide oxidoreductase, light-driven enzyme protochlorophyllide oxidoreductase, light-independent (dark) Pchlide oxidoreductase, light-independent NADPH:protochlorophyllide oxidoreductase, light-independent protochlorophyllide oxidoreductase, LIPOR, LPOR, NADPH Pchlide oxidoreductase, NADPH-Pchlide oxidoreductase, NADPH-protochlorophyllide oxidoreductase, NADPH-protochlorophyllide oxidoreductase (POR)-like protein, NADPH-protochlorophyllide oxidoreductase A, NADPH-protochlorophyllide oxidoreductase B, NADPH-protochlorophyllide reductase, NADPH2-protochlorophyllide oxidoreductase, NADPH: protochlorophyllide oxidoreductase, NADPH: protochlorophyllide oxidoreductase A, NADPH: protochlorophyllide oxidoreductase B, NADPH:Pchlide oxidoreductase, NADPH:Pchlide oxidoreductase A, NADPH:protochlorophyllide (Pchlide) oxidoreductase, NADPH:protochlorophyllide oxidoreductase, NADPH:protochlorophyllide oxidoreductase A, NADPH:protochlorophyllide oxidoreductase B, Os04g58200, Os10g0496900, OsPORA, OsPORB, PAR-A, Pchilde reductase, Pchlide oxidoreductase, PCR, POR, POR A, POR B, POR C, POR pPm1, POR pPm2, POR-A, POR-B, POR-C, POR-PChlide640, POR-PChlide650, POR1, POR2, POR3, PORA, PORB, PORC, protochlorophyllide oxidoreductase, protochlorophyllide oxidoreductase A, protochlorophyllide oxidoreductase B, protochlorophyllide oxidoreductase C, protochlorophyllide oxidoreductase POR-PChlide640, protochlorophyllide oxidoreductase POR-PChlide650, protochlorophyllide photooxidoreductase, protochlorophyllide reductase

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.33 protochlorophyllide reductase

Cloned

Cloned on EC 1.3.1.33 - protochlorophyllide reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
a genomic library is constructed, the vector pZERO is used
barley POR expressed in Escherichia coli as a beta-galactosidase fusion protein
-
cDNA library constructed in pBR322, full-length cDNA cloned, cDNA inserts subcloned in the M13 phage and expressed in Escherichia coli
-
cDNAs and/or gens encoding POR, also referred to as LPCR or PCR in some studies isolated
-
cloned and overproduced in Escherichia coli with a hexahistidine tag at the N-terminus
-
cloning of the gene encoding protochlorophyllide reductase
-
cloning of the nuclear gene
-
expressed in Escherichia coli
expressed in Escherichia coli BL21(DE3) cells
Thermosynechococcus vestitus
expressed in Escherichia coli BL21(DE3) Codon Plus RIL cells
-
expressed in Escherichia coli BL21(DE3) pLysS cells
expressed in Escherichia coli strain SG13009
-
expressed in Rhodobacter capsulatus SB1003, JDA, JDB and ZY5
-
expression in Escherichia coli BL21
expression in Escherichia coli cells
expression in Escherichia coli with a Strep-tag
expression in Rhodobacter capsulatus mutant deficient in Bchl biosynthesis. The NADPH:protochlorophyllide oxidoreductase is integrated in the porphyrin biosynthesis network and its activity leads to the formation of photosynthetic chlorophyll proteins
-
for sequencing
full-length cDNA, coding for PCR
-
into the vector pQE30
-
mature pea POR cloned into the pAlter-1 mutagenesis vector, expressed in Escherichia coli JM109
-
optimized and expressed in Escherichia coli
Thermosynechococcus vestitus
overexpressed as a fusion with maltose-binding protein in Escherichia coli using expression plasmid pKK233-2
-
overexpressed in Escherichia coli as a fusion protein with the maltose-binding protein
-
overexpression in Escherichia coli
Thermosynechococcus vestitus
-
overexpression in Escherichia coli as a fusion protein with maltose-binding protein
-
overexpression in Escherichia coli as a His6-tagged protein
POR C cloned and expressed in Escherichia coli
-
subcloned into the expression vector pRSETA and recombinantly expressed in Escherichia coli SoluBL21
Thermosynechococcus vestitus
the coding regions of LPOR (amino acids 35-397) is amplified from pBDCrPCYA1-FDBR vector, cloned into pGADT7 vector, and introduced into Y187 yeast cells, recombinant expression of MBP-tagged enzyme LPOR. Yeast two-hybrid and pull down assay analyses of protein-protein interaction
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells
yes into the vector pTYB for expression in Escherichia coli BL21DE3 cells, for the construction of transgenic plants pDONR/ZeO and pGWB2 vectors are used