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1.3.1.20: trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

This is an abbreviated version!
For detailed information about trans-1,2-dihydrobenzene-1,2-diol dehydrogenase, go to the full flat file.

Word Map on EC 1.3.1.20

Reaction

trans-1,2-Dihydrobenzene-1,2-diol
+
NADP+
=
catechol
+
NADPH
+
H+

Synonyms

AKR1C1, AKR1C2, DD, DDH, DDH1, DDH2, dehydrogenase, trans-1,2-dihydrobenzene-1,2-diol, DHDH, dihydrodiol dehydrogenase, More

ECTree

     1 Oxidoreductases
         1.3 Acting on the CH-CH group of donors
             1.3.1 With NAD+ or NADP+ as acceptor
                1.3.1.20 trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

Crystallization

Crystallization on EC 1.3.1.20 - trans-1,2-dihydrobenzene-1,2-diol dehydrogenase

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CRYSTALLIZATION (Commentary)
ORGANISM
UNIPROT
LITERATURE
crystals are obtained at 19.9°C in a culture plate via the vapour-diffusion method, crystal structure of dimeric dihydrodiol dehydrogenase complexed with the inhibitor isoascorbic acid is determined at 2.59 A resolution
dimeric apoenzyme (at 2.0 A resolution) and enzyme-inhibitor complex (at 2.9 A resolution), crystals are grown using droplets consisting of the dihydrodiol dehydrogenase/NADPH solution mixed with a matching volume containing 1.5 M ammonium phosphate and 0.1 M sodium citrate at pH 5.6, tertiary structure is formed by a classical dinucleotide binding fold comprising of 2 beta-alpha-beta-alpha-beta motifs at the N-terminus and an eight-stranded, predominantly antiparallel beta-sheet at the C-terminus
vapour diffusion method with 2 M ammonium phosphate and 0.1 M sodium citrate buffer (pH 5.6) and 20% glycerol
-
in complex with isoascorbic acid, vapour diffusion method with 2 M ammonium sulfate, 0.1 M sodium citrate buffer pH 5.0, at 20°C
-
vapour diffusion at 295 K with NADPH, growth for 2 days, buffer A: 1.5 M ammoinum phosphate, 0.1 M sodium citrate, pH 5.6 + buffer B: 10 mM Tris-HCl, 2 mM 2-mercaptoethanol, pH 8.5, preliminary x-ray structure analysis
-
complexed with NADP+ and testosterone, 10fold molar excess of NADP+, 'waterbug' dialysis method in 10 mM potassium phosphate buffer, pH 7.0, containing 1 mM EDTA, 1 mM 2-mrcapthoethanol, 5% acetonitrile and 0.2 mM testosterone, and crystallization in presence of polethylene glycol 8000 in hanging drops at room temperature, single crystals
-
cryocrystallization: crystals were soaked in 21% polyethylene glycol 8000, 0.1 M sodium cacodylate, pH 6.4, 1.25 mM NADP+, 0.1 mM testosterone and 15% 2-methyl-2,4-pentanediol as cryoprotectant
-
structure determination, cofactor and substrate binding site, and model building
-
vapour diffusion at 22°C in 100 mM sodium citrate, polyethylene glycol 6000 25%, 120 mM ammonium sulfate, pH 5.8, x-ray analysis
-