1.3.1.14: dihydroorotate dehydrogenase (NAD+)
This is an abbreviated version!
For detailed information about dihydroorotate dehydrogenase (NAD+), go to the full flat file.
Word Map on EC 1.3.1.14
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1.3.1.14
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pyrimidine
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flavin
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brequinar
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medicine
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l-dihydroorotate
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fmn
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lactis
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o2-sensitive
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mononucleotide
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photons
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bifidobacterium
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bifidum
-
berghei
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ubiquinone
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carbamyl
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half-reaction
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nutrition
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pharmacology
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1.3.3.1
- 1.3.1.14
- pyrimidine
- flavin
- brequinar
- medicine
- l-dihydroorotate
- fmn
- lactis
-
o2-sensitive
- mononucleotide
-
photons
- bifidobacterium
- bifidum
- berghei
- ubiquinone
-
carbamyl
-
half-reaction
- nutrition
- pharmacology
-
1.3.3.1
Reaction
Synonyms
b-type dihydroorotate dehydrogenase, DHO dehydrogenase, DHODase, DHOdehase A, dihydro-orotic dehydrogenase, dihydroorotate dehydrogenase, dihydrorotate dehydrogenase B, L-5,6-dihydro-orotate:NAD oxidoreductase, More, orotate reductase, reductase, orotate
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1.3.1.14 dihydroorotate dehydrogenase (NAD+)Advanced search results
results (
results (Substrates Products
Substrates Products on EC 1.3.1.14 - dihydroorotate dehydrogenase (NAD+)
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SUBSTRATE 
PRODUCT
REACTION DIAGRAM
ORGANISM
UNIPROT
COMMENTARY
(Substrate)
(Substrate)
LITERATURE
(Substrate)
(Substrate)
COMMENTARY
(Product)
(Product)
LITERATURE
(Product)
(Product)
Reversibility
r=reversible
ir=irreversible
?=not specified
r=reversible
ir=irreversible
?=not specified
(S)-dihydroorotate + 2,6-dichlorophenolindophenol
orotate + reduced 2,6-dichlorophenolindophenol
-
-
-
?
5-fluorodihydroorotate + NAD+
5-fluoroorotate + NADH + H+
-
more active substrate for reverse reaction than orotate
-
-
r
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
different specific activities with potassium ferricyanide, O2, fumarate and NAD+ as electron acceptors for PyrDI and the holoenzyme
-
?
(S)-dihydroorotate + acceptor
orotate + reduced acceptor
-
fourth step in UMP-biosynthesis
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
mechanism and pH-dependence of reaction
-
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
anti-elimination of hydrogen from (S)-dihydroorotate
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
equilibrium favours direction of orotate reduction
-
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
equilibrium favours direction of orotate reduction
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
equilibrium favours direction of orotate reduction
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
equilibrium favours direction of orotate reduction
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
equilibrium favours direction of orotate reduction
orotate is identical with 4-carboxyuracil
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?, r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
substrate is oxidized by NAD+ and oxygen
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
substrate is oxidized by NAD+ and oxygen
lipoic acid is no substitute for orotate
?
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
mechanism of dehydrogenation, mechanism of electron transfer: L-dihydroorotate transfers a pair of electrons to FMN via iron-sulfur cluster via FAD to NAD+
-
?, r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
mechanism of dehydrogenation, mechanism of electron transfer: L-dihydroorotate transfers a pair of electrons to FMN via iron-sulfur cluster via FAD to NAD+
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
?, r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
NAD+ as ultimate electron acceptor
-
r
(S)-dihydroorotate + NAD+
orotate + NADH + H+
-
at higher pH values reaction favours direction of dihydroorotate oxidation, whereas at lower pH values direction of orotate reduction is favoured
-
r
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: NAD+
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
acceptor: menadione
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
acceptor: NAD+ for pyrDa gene product, fumarate for pyrDb gene product
-
-
?
dihydroorotate + acceptor
orotate + reduced acceptor
-
activity measurement in permeabilized Ehrlich ascites tumor cells, acceptor: nitroblue tetrazolium
-
-
?
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
dihydroorotate + NAD+
orotate + NADH + H+
-
-
breakdown of orotate
r
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
dihydroorotate + NAD+
orotate + NADH + H+
-
biosynthesis of pyrimidines
-
?
dihydroorotate + NAD+
orotate + NADH + H+
-
enzyme presumably functions mainly in the metabolic synthesis of pyrimidines
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r
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
dihydroorotate + NAD+
orotate + NADH + H+
-
fourth step and sole redox reaction in the pyrimidine de novo biosynthetic pathway
-
r
additional information
?
-
-
not: uracil, cytosine, 5-methylcytosine, thymine as substrates
-
-
?
additional information
?
-
-
not: uracil, cytosine, 5-methylcytosine, thymine as substrates
-
-
?
additional information
?
-
-
3-acetylpyridine-NAD+ is a more effective oxidant for dihydroorotate than NAD+, methylene blue can serve as oxidant, but not cytochrome c
-
-
?
additional information
?
-
-
NAD+ binding domain in the beta subunit of enzyme, Cys-135 plays a catalytic role and Lys-48 is important for orienting the substrate in the active site and is able to interact with FMN
-
-
?
additional information
?
-
-
O2, fumarate, and NADP+ do not serve as electron acceptors
-
-
?
additional information
?
-
O2, fumarate, and NADP+ do not serve as electron acceptors
-
-
?
