1.3.1.12: prephenate dehydrogenase

This is an abbreviated version!
For detailed information about prephenate dehydrogenase, go to the full flat file.

Word Map on EC 1.3.1.12

1.3.1.12

l-tyrosine

h-protein

arogenate

hyperglycinemia

nonketotic

4-hydroxyphenylpyruvate

aminomethyltransferase

dahp

3-deoxy-d-arabino-heptulosonate

cyclohexadienyl

7-phosphate

dimethylglycine

tetrahydrofolate-dependent

glycine-cleavage

aminomethyl

folate-binding

Reaction

Synonyms

AceF, AroQ, bifunctional T-protein, chorismate mutase-prephenate dehydratase, chorismate mutase-prephenate dehydrogenase bifunctional enzyme, chorismate mutase-T:prephenate dehydrogenase bifunctional enzyme, Chorismate mutase/prephenate dehydratase, CM-PD, CM-TyrAp, CM/PDT/PDHG, CMPD, dehydrogenase, prephenate, hydroxyphenylpyruvate synthase, PD, PDH, pdhE-1, PDHG, T-protein, TYR1, tyrA, TyrA dehydrogenase, TyrAp

ECTree

1 Oxidoreductases

1.3 Acting on the CH-CH group of donors

1.3.1 With NAD⁺ or NADP⁺ as acceptor

1.3.1.12 prephenate dehydrogenase

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Results	in table
8	Activating Compound
11849	AA Sequence
2	Application
3	CAS Registry Number
21	Cloned(Commentary)
37	Cofactor
5	Crystallization (Commentary)
4	General Information
21	General Stability
1	IC50 Value
105	Inhibitors
7	kcat/KM [mM/s]
26	Ki Value [mM]
82	KM Value [mM]
6	Metals/Ions
33	Molecular Weight [Da]
60	Natural Substrates/ Products (Substrates)
277	Organism
8	Pathway
21	PDB ID
11	pH Optimum
3	pH Range
1	pI Value
1	Posttranslational Modification
18	Protein Variants
36	Purification (Commentary)
3	Reaction
4	Reaction Type
80	Reference
29	Specific Activity [micromol/min/mg]
13	Storage Stability
140	Substrates and Products (Substrate)
18	Subunits
79	Synonyms
1	Systematic Name
11	Temperature Optimum [°C]
4	Temperature Range [°C]
6	Temperature Stability [°C]
51	Turnover Number [1/s]

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Cloned on EC 1.3.1.12 - prephenate dehydrogenase

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3 entries

expressed in Escherichia coli

3 entries

expressed in Escherichia coli BL21(DE3) cells

Haemophilus influenzae

740113

expression in Escherichia coli

2 entries

expression of His-tagged enzyme in Escherichia coli

Haemophilus influenzae

P43902

710738

gene tyrA, genetic mapping of chorismate mutase and prephenate dehydrogenase activity, expression of the wild-type enzyme, the 2 activity domains, and gene fragments exhibiting either chorismate mutase or prephenate dehydrogenase activity as His-tagged proteins and peptides in strain BL21(DE3), respectively

Escherichia coli

655472

genetic separability of chorismate mutase and prephenate dehydrogenase in Escherichia coli

Escherichia coli

390516

into tyrA-pET30a and expressed in Escherichia coli BL21(DE3) as His-rMtPDH

Mycobacterium tuberculosis

676965

into vector pET-15b, expression as His-tagged protein in Escherichia coli

Aquifex aeolicus

677010

into vector pET15b and expressed in Escherichia coli BL21-Gold (DE3)

Aquifex aeolicus

674545

monofunctional prephenate dehydrogenase, expressed in Escherichia coli

Pantoea agglomerans

390554

mutants cloned into pZE21-MCS1 and transformed to Escherichia coli DH5alpha, wild-type and mutants subcloned into vector pET-30XA/LIC and expressed in Escherichia coli BL21(DE3)

Escherichia coli

671431

overexpressed in Escherichia coli

Archaeoglobus fulgidus

overexpression

T7 expression system