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1.23.1.4: (-)-lariciresinol reductase

This is an abbreviated version!
For detailed information about (-)-lariciresinol reductase, go to the full flat file.

Reaction

(+)-secoisolariciresinol
+
NADP+
=
(-)-lariciresinol
+
NADPH
+
H+

Synonyms

(–)-pinoresinol-(–)-lariciresinol reductase, bi-functional pinoresinol/lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase, bifunctional pinoresinol-lariciresinol reductase 1, LuPLR1, More, pinoresinol lariciresinol reductase, pinoresinol reductase, pinoresinol reductase 1, pinoresinol-lariciresinol reductase, pinoresinol-lariciresinol reductase-1, pinoresinol/lariciresinol reductase, PLR, PLR-Lu1, PLR-Tp1, PLR-Tp2, PLR1, PrR1, SS-pinoresinol-SS-lariciresinol reductase

ECTree

     1 Oxidoreductases
         1.23 Reducing C-O-C group as acceptor
             1.23.1 With NADH or NADPH as donor
                1.23.1.4 (-)-lariciresinol reductase

Cloned

Cloned on EC 1.23.1.4 - (-)-lariciresinol reductase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
expressed in BL21 (DE3)-RIL cells
expressed in Escherichia coli B834-DE3 cells
expressed in Escherichia coli BL21(DE3)pLysS cells
-
expressed in Escherichia coli Nova Blue cells
-
expressed in Escherichia coli strain HB101
-
gene LuPLR1, quantitative RT-PCR expression analysis of LuPLR1
gene pinZ, recombinant expression in Arabidopsis thaliana under the control of the cauliflower mosaic virus 35S promoter, pinZ expression causes dynamic metabolic changes in stems, but not in roots and leaves. Accumulation of the glucoside of secoisolariciresinol appears to be elevated in the transgenic plant. Expression of pinZ influenced the metabolisms of lignan and glucosinolates but not so much of neolignans such as guaiacylglycerol-8-O-4'-feruloyl ethers, recombinant enzyme tissue expression pattern in plant seedlings
gene PLR_Lu1, DNA and amino acid sequence determination and analysis, quantitative real-time RT-PCR enzyme expression analysis
-
gene PrR1, PrR1 is co-expressed with many characterized genes involved in secondary cell wall biosynthesis, gene co-expression networks for Arabidopsis thaliana PrR1 and PrR2, overview. PrR1 is regulated by the secondary cell wall transcription factors SND1 and MYB46
the plasmid is transferred into the disarmed Agrobacter tumefaciens strain GV3101 by triparental mating with Escherichia coli strain HB101 and then introduced into Linum usitatissimum (cv. Barbara) transgenic plants
-
the promoter of one pinoresinol-lariciresinol reductase gene LuPLR1 is fused to a beta-glucuronidase (GUS) reporter gene, and the spatiotemporal regulation of LuPLR1 gene expression via this promoter in flaxseed is determined by histochemical and activity assays of GUS, recombinant expression of the promoter construct in transgenic flaxseed via transformation with Agrobacterium tumefaciens, quantitative RT-PCR expression analysis