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1.21.99.3: thyroxine 5-deiodinase

This is an abbreviated version!
For detailed information about thyroxine 5-deiodinase, go to the full flat file.

Word Map on EC 1.21.99.3

Reaction

3,3',5'-triiodo-L-thyronine
+
Iodide
 +
acceptor
 +
H+
=
L-thyroxine
 +
reduced acceptor

Synonyms

5'-deiodinase, D3, deiodinase, deiodinase 3, deiodinase, thyroxine 5-, deiodinase-3, diiodothyronine 5'-deiodinase, Dio, Dio3, Dio3a, Dio3b, EC 1.97.1.11, ID-3, iodothyronine 5-deiodinase, iodothyronine deiodinase type III, iodothyronine inner ring monodeiodinase, iodothyronine monodeiodinase, ITHD, T4-5'-deiodinase, T4-5-deiodinase, thyroxine 5-deiodinase, type 3 deiodinase, type 3 iodothyronine 5-deiodinase, type 3 iodothyronine deiodinase, type III deiodinase, type III iodothyronine deiodinase, type-3 deiodinase, zD3

ECTree

     1 Oxidoreductases
         1.21 Catalysing the reaction X-H + Y-H = X-Y
             1.21.99 With unknown physiological acceptors
                1.21.99.3 thyroxine 5-deiodinase

Cloned

Cloned on EC 1.21.99.3 - thyroxine 5-deiodinase

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CLONED (Commentary)
ORGANISM
UNIPROT
LITERATURE
DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, contains a putative Sec insertion sequence element at 3' UTR, functional expression in COS-1 cells
expressed in Escherichia coli Rosetta2 cells
expressed in Xenopus laevis oocytes
expression in oocytes from Xenopus laevis
expression of RNAi to knock down D3 in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes
-
expression of RNAi to knock down type 3 deiodinase in transient transfection experiments using G2N2C, Tb3A, and wild type keratinocytes
-
isozyme type III, expression of wild-type and active site mutants in COS cells, 50fold higher expression level for the mutants than for the wild-type enzyme
isozyme type III, via RT-PCR from cerebellum mRNA, expression in bacteria
-
screening of neonatal skin cDNA library, DNA sequence determination and analysis, isozyme type III, an in-frame TGA codon encodes a selenocysteine residue, functional translation in Xenopus laevis oocytes, catalytis properties of recombinant and native enzyme are identical
the enzyme is a selenoenzyme encoded by the DIO3 gene, localized on chromosome 14q32, in humans. The DIO3 genomic structure contains a single exon and, at the 3'-UTR, a specific RNA structure, named SECIS (selenocysteine insertion element), that is crucial for the insertion of the selenocysteine residue and for maximal enzymatic catalytic efficiency. The DIO3 gene is imprinted, with preferential expression of the paternal allele. It belongs to a cluster of imprinted regions, at the DLK1-DIO3 locus
transfection in HEK 293 cells
-